Register: Virtual PSC Training Spring 2021

Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells (iPS Cells)
Maintaining Human Pluripotent Stem Cells (hPSCs)
Differentiation of iPS Cells
Acceptance into the course is based upon review by STEMCELL Technologies. You will be notified within one week of submission on your status. Payment is required in order to confirm your seat in the course. If registration is full, you will be added to our next available session. Space is limited to ensure personalized instruction. Please register early to guarantee your seat.

Virtual Pluripotent Stem Cell Training Hosted from Vancouver, Canada: April 19 - 23, 2021

Human pluripotent stem cell (hPSC) research is an expanding field with the potential to change how human diseases are studied and treated. With this five-day, part-time course, you can learn the techniques and protocols to help get your hPSC research started. Guided by experts from STEMCELL Technologies, you will gain skills to successfully derive and maintain high-quality human induced pluripotent stem (iPS) cells from somatic cells and differentiate them toward specialized cell types.


DATE & FORMAT
April 19-23, 2021
Virtual live stream


PRICE
5-day option: 625 USD / 800 CDN / 500 EUR / 450 GBP*
2-day option: 200 USD / 250 CDN / 150 EUR / 160 GBP* (covers hPSC maintenance only)

*The course will be charged in your regional currency.

Space is limited to ensure personalized instruction. Please register early to guarantee your seat.

AGENDA

VIRTUAL Reprogramming, Maintenance, and Differentiation of hPSCs Course Agenda

PST (EST)
Monday April 19, 2021
Day 1: hPSC Maintenance Day 1
8:00 - 8:10
(11:00 - 11:10)
Virtual Welcome & Introductions
8:10 - 10:00
(11:10 - 13:00)
Lecture 1: Isolation and Reprogramming of Somatic Cells into iPS Cells
10:00 - 10:30
(13:00 - 13:30)
Break / Lunch
10:30 - 11:30
(13:30 - 14:30)
Virtual Practical Session 1: Morphology and Evaluating the Quality of hPSC Culture
  • Observe time course of hPSC cultures
  • Assessing high quality hPSC colony morphology
11:20 - 11:30
(14:20 - 14:30)
Break
11:30 - 12:15
(14:30 - 15:15)
Virtual Practical Session 2: Plate Coating
  • Plate coating with Matrigel® or Vitronectin XF™
12:15 - 12:45
(15:15 - 15:45)
Q & A Wrap Up Session
Tuesday April 20, 2021
Day 2: hPSC Maintenance Day 2
8:00 - 8:05
(11:00 - 11:05)
Virtual Welcome & Introductions
8:05 - 9:00
(11:05 - 12:00)
Virtual Lecture 2: Characterization of hPSC Cell Quality
9:00 - 9:30
(12:00 - 12:30)
Break / Lunch
9:30 - 11:30
(12:30 - 14:30)
Virtual Lab Session 3: Passaging and Long-Term Maintenance of hPSCs

  • Hands-on practice with enzyme-free passaging of hPSCs using Gentle Cell Dissociation Reagent and ReLeSR™
11:30 - 11:45
(14:30 - 14:45)
Break
11:45 - 12:30
(14:45 - 15:30)
Virtual Lab Session 4: Cryopreservation and Thawing of hPSCs
12:30 - 12:45
(15:30 - 15:45)
Q & A Wrap-Up Session
Wednesday April 21, 2020
Day 3: Reprogramming of Somatic Cells into iPS Cells
8:00 - 10:00
(11:00 - 13:00)
Virtual Lecture 3: Isolation and Reprogramming of Somatic Cells into iPS Cells
10:00 - 10:30
(13:00 - 13:30)
Lunch
10:30 - 12:15
(13:30 - 15:15)
Virtual Practical Session 5: Selection and Picking of iPS Cell Colonies
  • Demonstrate transfection of human fibroblasts using ReproRNA™-OKSGM
  • Observe reprogrammed cells cultured in ReproTeSR™
  • Demonstrate Selection and Picking of iPS Cell Colonies
12:15 - 12:30
(15:15 - 15:30)
Q & A Wrap-Up Session
  • PollEV

Thursday April 22, 2021
Day 4: Neural Differentiation Day 1
8:00 - 9:30
(11:00 - 12:30)
Virtual Lecture/Lab: Induction of hPSCs to Neural Progenitors - Part 1
9:30 - 10:00
(12:30 - 1:00)
Break / Lunch
10:00 - 11:00
(1:00 - 2:00)
Virtual Lecture/Lab: Induction of hPSCs to Neural Progenitors - Part 2
11:00 - 11:15
(2:00 - 2:15)
Break
11:15 - 12:15
(2:15 - 3:15)
Virtual Lecture: Differentiation of Neural Progenitors to Mature Neural Cell Types
12:15 - 12:30
(3:15 - 3:30)
Q & A Wrap-Up Session
Friday April 23, 2021
Day 5: Neural Differentiation Day 2
8:00 - 9:30
(11:00 - 12:30)
Virtual Lecture/Lab: Differentiation of hPSCs to Unpatterned Cerebral Organoids
9:30 - 10:00
(12:30 - 1:00)
Break / Lunch
10:00 - 11:00
(1:00 - 2:00)
Virtual Lecture: Characterization of Cerebral Organoids & Example Workflow for Generating Gene-Edited Cerebral Organoids
11:00 - 11:15
(2:00 - 2:15)
Break
11:15 - 12:15
(2:15 - 3:15)
Virtual Lecture: Differentiation of hPSCs to Patterned Dorsal & Ventral Forebrain Organoids
12:15 - 12:30
(3:15 - 3:30)
Q & A Wrap-Up Session
  • Neural Quiz TBD

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