Register: Virtual PSC Training Spring 2021

What is your level of cell culture experience?

Choose the topic(s) that you are most interested in learning about:

Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells (iPS Cells)
Maintaining Human Pluripotent Stem Cells (hPSCs)
Differentiation of iPS Cells

Acceptance into the course is based upon review by STEMCELL Technologies. You will be notified within one week of submission on your status. Payment is required in order to confirm your seat in the course. If registration is full, you will be added to our next available session. Space is limited to ensure personalized instruction. Please register early to guarantee your seat.

Virtual Pluripotent Stem Cell Training Hosted from Vancouver, Canada: April 19 - 23, 2021

Human pluripotent stem cell (hPSC) research is an expanding field with the potential to change how human diseases are studied and treated. With this five-day, part-time course, you can learn the techniques and protocols to help get your hPSC research started. Guided by experts from STEMCELL Technologies, you will gain skills to successfully derive and maintain high-quality human induced pluripotent stem (iPS) cells from somatic cells and differentiate them toward specialized cell types.

DATE & FORMAT
April 19-23, 2021
Virtual live stream

PRICE
5-day option: 625 USD / 800 CDN / 500 EUR / 450 GBP*
2-day option: 200 USD / 250 CDN / 150 EUR / 160 GBP* (covers hPSC maintenance only)

*The course will be charged in your regional currency.

Space is limited to ensure personalized instruction. Please register early to guarantee your seat.

AGENDA

VIRTUAL Reprogramming, Maintenance, and Differentiation of hPSCs Course Agenda

PST (EST)
Monday April 19, 2021

Day 1: hPSC Maintenance Day 1

8:00 - 8:10
(11:00 - 11:10)

Virtual Welcome & Introductions

8:10 - 10:00
(11:10 - 13:00)

Lecture 1: Isolation and Reprogramming of Somatic Cells into iPS Cells

10:00 - 10:30
(13:00 - 13:30)

Break / Lunch

10:30 - 11:30
(13:30 - 14:30)

Virtual Practical Session 1: Morphology and Evaluating the Quality of hPSC Culture

Observe time course of hPSC cultures
Assessing high quality hPSC colony morphology

11:20 - 11:30
(14:20 - 14:30)

Break

11:30 - 12:15
(14:30 - 15:15)

Virtual Practical Session 2: Plate Coating

Plate coating with Matrigel® or Vitronectin XF™

12:15 - 12:45
(15:15 - 15:45)

Q & A Wrap Up Session

Tuesday April 20, 2021

Day 2: hPSC Maintenance Day 2

8:00 - 8:05
(11:00 - 11:05)

Virtual Welcome & Introductions

8:05 - 9:00
(11:05 - 12:00)

Virtual Lecture 2: Characterization of hPSC Cell Quality

9:00 - 9:30
(12:00 - 12:30)

Break / Lunch

9:30 - 11:30
(12:30 - 14:30)

Virtual Lab Session 3: Passaging and Long-Term Maintenance of hPSCs

Hands-on practice with enzyme-free passaging of hPSCs using Gentle Cell Dissociation Reagent and ReLeSR™

11:30 - 11:45
(14:30 - 14:45)

Break

11:45 - 12:30
(14:45 - 15:30)

Virtual Lab Session 4: Cryopreservation and Thawing of hPSCs

12:30 - 12:45
(15:30 - 15:45)

Q & A Wrap-Up Session

Wednesday April 21, 2020

Day 3: Reprogramming of Somatic Cells into iPS Cells

8:00 - 10:00
(11:00 - 13:00)

Virtual Lecture 3: Isolation and Reprogramming of Somatic Cells into iPS Cells

10:00 - 10:30
(13:00 - 13:30)

Lunch

10:30 - 12:15
(13:30 - 15:15)

Virtual Practical Session 5: Selection and Picking of iPS Cell Colonies

Demonstrate transfection of human fibroblasts using ReproRNA™-OKSGM
Observe reprogrammed cells cultured in ReproTeSR™
Demonstrate Selection and Picking of iPS Cell Colonies

12:15 - 12:30
(15:15 - 15:30)

Q & A Wrap-Up Session

PollEV

Thursday April 22, 2021

Day 4: Neural Differentiation Day 1

8:00 - 9:30
(11:00 - 12:30)

Virtual Lecture/Lab: Induction of hPSCs to Neural Progenitors - Part 1

9:30 - 10:00
(12:30 - 1:00)

Break / Lunch

10:00 - 11:00
(1:00 - 2:00)

Virtual Lecture/Lab: Induction of hPSCs to Neural Progenitors - Part 2

11:00 - 11:15
(2:00 - 2:15)

Break

11:15 - 12:15
(2:15 - 3:15)

Virtual Lecture: Differentiation of Neural Progenitors to Mature Neural Cell Types

12:15 - 12:30
(3:15 - 3:30)

Q & A Wrap-Up Session

Friday April 23, 2021

Day 5: Neural Differentiation Day 2

8:00 - 9:30
(11:00 - 12:30)

Virtual Lecture/Lab: Differentiation of hPSCs to Unpatterned Cerebral Organoids

9:30 - 10:00
(12:30 - 1:00)

Break / Lunch

10:00 - 11:00
(1:00 - 2:00)

Virtual Lecture: Characterization of Cerebral Organoids & Example Workflow for Generating Gene-Edited Cerebral Organoids

11:00 - 11:15
(2:00 - 2:15)

Break

11:15 - 12:15
(2:15 - 3:15)

Virtual Lecture: Differentiation of hPSCs to Patterned Dorsal & Ventral Forebrain Organoids

12:15 - 12:30
(3:15 - 3:30)

Q & A Wrap-Up Session

Neural Quiz TBD