Far-Red Nuclear Stain is a cell-permeable, far-red fluorogenic dye that is recommended for staining and visualization of cell nuclei in live or fixed cell cultures. As a fluorogenic dye, Far-Red Nuclear Stain exhibits low background, as it only becomes fluorescent upon binding DNA. Far-red dyes are also advantageous over traditional blue dyes, as they display less autofluorescence and phototoxicity by avoiding DNA damage induced by exposure to UV light. Upon binding DNA, Far-Red Nuclear Stain emits a far-red signal that can be viewed using fluorescence microscopy, fluorescent microplate, or flow cytometry equipped with a standard cyanine-5 (Cy5) filter set. Far-Red Nuclear Stain is also compatible in multi-color staining of live or fixed cells.