ErythroClear™ Red Blood Cell Depletion Reagent Kit
Red blood cell depletion kit for small-volume cord blood samples
Overview
The ErythroClear™ Red Blood Cell Depletion Reagent Kit (Catalog #01738) contains ErythroClear™ reagent only, and is suitable as a refill for labs that already have an ErythroClear™ magnet. The ErythroClear™ magnet is required for use of the ErythroClear™ reagent.
Data Figures
Figure 1. RBC Depletion of Whole Cord Blood Sample Using ErythroClear™
Representative Flow Cytometry Plots of Whole Cord Blood Samples Stained for GlyAB and CD45 Before and After RBC Depletion with ErythroClear™
Figure 2. Improved Visualization of Cord Blood-Derived Colonies in CFU Assays After RBC Depletion with ErythroClear™
Representative STEMvision™ images of cord blood CFU assays (A) with and (B) without ErythroClear™ RBC depletion prior to plating. The expanded orange circles illustrate that it is easier to identify small or diffuse colonies in MethoCult™ medium that is free of RBC background (A vs. B).
Figure 3. ErythroClear™ Does Not Change the Frequency of CFUs in Cord Blood Samples
The frequency of CFUs in Erythroclear™ depleted (y-axis) and non-depleted (x-axis) cord blood samples are shown. One half of each cord blood sample was processed with ErythroClear™ prior to plating in MethoCult™ H4434 Classic medium. The other half of each sample was plated without RBC depletion. The total number of CFUs in the RBC depleted and non-depleted samples were scored manually after 14 days of culture (n = 32 fresh (triangle) and 8 frozen (circle) cord blood samples, solid line = linear regression best-fit, dotted lines define 95% confidence intervals). The frequency of CFUs in each sample before and after removal of RBCs using ErythroClear™ is highly correlated (coefficient of determination R2 = 0.83). The average recovery of total nucleated cells (TNCs) after RBC depletion with ErythroClear™ was 75%.
Figure 4. The Frequency of CD34+ Cells in Cord Blood is Not Changed After RBC Depletion with ErythroClear™
Shown are the percentages of GlyAB-CD45+ cells that are CD34+ in cord blood samples before (light gold) and after (gold) RBC depletion with ErythroClear™. There was no significant difference in the frequency of CD34+ cells in this population before and after RBC depletion for each sample (p>0.01). The recovery of CD34+ cells after RBC depletion with ErythroClear™ was 70%. Additionally the frequency of GlyAB-CD45+ that are ALDHbright (data not shown) was not significantly different after ErythroClear™ treatment (p>0.01), and the recovery of these cells was 66%. (n = 8 frozen whole cord blood samples, processed and analyzed separately in triplicate, columns with error bars represent mean ± S.D.).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (1)
Abstract
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
