EasySep™ Release Human CD4 Positive Selection Kit
Immunomagnetic positive selection kit using particle release technology
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Required Products
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EasySep™ MagnetMagnet for column-free immunomagnetic separation
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EasySep™ BufferCell separation buffer
Overview
The EasySep™ Release Human CD4 Positive Selection Kit is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
Advantages
• Highly purified human CD4+ cells isolated in less than 30 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
- EasySep™ Release Human CD4 Positive Selection Kit (Catalog #17752)
- EasySep™ Release Human CD4 Positive Selection Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™ 50201, 1 mL
- EasySep™ Release Buffer (Concentrate), 3 x 1 mL
- RoboSep™ Human CD4 Positive Selection Kit (Catalog #17752RF)
- EasySep™ Release Human CD4 Positive Selection Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™, 1 mL
- EasySep™ Release Buffer (Concentrate), 3 x 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
- EasySep™ EasyTube™-14 (Catalog #20128)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ Magnet (Catalog #18102)
• EasyEights™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Brand
EasySep, RoboSep
Area of Interest
Cell Therapy, Immunology
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
RoboSep™ Release Human CD4 Positive Selection Kit
|
Catalog #
17752RF |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
EasySep™ Release Human CD4 Positive Selection Kit
|
Catalog #
17752 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
RoboSep™ Release Human CD4 Positive Selection Kit
|
Catalog #
17752RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
RoboSep™ Release Human CD4 Positive Selection Kit
|
Catalog #
17752RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Release Human CD4 Positive Selection Kit
|
Catalog #
17752 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Release Human CD4 Positive Selection Kit
|
Catalog #
17752 |
Lot #
All |
Language
English |
Educational Materials (10)
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Wallchart
Production of Chimeric Antigen Receptor T Cells
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
The Immune Response to HIV Poster
Video
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Scientific Poster
Releasable RapidSpheres Enable Immunomagnetic Purification of Highly Viable and Functional Immune Cells from Complex Tissues in Less Than 30 Minutes
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Data and Publications
Data
Starting with a single-cell suspension of human PBMCs, the CD4+ T cell content of the isolated fraction is typically 96.1 ± 4.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 24.0% and 98.1%, respectively.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
