EasySep™ Rat IgM Depletion Kit

Immunomagnetic depletion kit

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Rat IgM Depletion Kit

Immunomagnetic depletion kit

From: 933 USD
Catalog #
18644_C
Immunomagnetic depletion kit

Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 90% purity (≤ 10% IgM+)

  • Isolated cells are untouched

What's Included

  • EasySep™ Rat IgM Depletion Kit (Catalog #18644)
    • EasySep™ Rat IgM Depletion Cocktail, 1 mL
    • Anti-Rat CD32 Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
  • RoboSep™ Rat IgM Depletion Kit (Catalog #18644RF)
    • EasySep™ Rat IgM Depletion Cocktail, 1 mL
    • Anti-Rat CD32 Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ Rat IgM Depletion Kit is designed to deplete IgM+ cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by positive selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Rat
Sample Source
Lymph Node, Other, Spleen, Whole Blood
Selection Method
Depletion
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Starting with a single-cell suspension of splenocytes from a Sprague Dawley rat, the IgM content of the final isolated fraction is typically 8.0 ± 4.1%. In the above example, the purities of the start and final depleted fractions are 55.7% and 8.7%, respectively (1.6 log depletion).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
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Language
Catalog #
18644
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All
Language
English
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
18644
Lot #
All
Language
English
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Safety Data Sheet 2
Catalog #
18644
Lot #
All
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English
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Safety Data Sheet 3
Catalog #
18644
Lot #
All
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English
Document Type
Safety Data Sheet 1
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
18644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
18644RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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