EasySep™ Human EGFR Positive Selection Kit

Kit for immunomagnetic positive selection of human EGFR+ cells

EasySep™ Human EGFR Positive Selection Kit

Kit for immunomagnetic positive selection of human EGFR+ cells

From: 795 USD
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Kit for immunomagnetic positive selection of human EGFR+ cells
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Product Advantages


  • Fast and easy-to-use

  • Up to 98% purity

  • No columns required

What's Included

  • EasySep™ Human EGFR Positive Selection Kit ( Catalog #100-1131)
    • EasySep™ Human EGFR Positive Selection Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 x 1 mL

Overview

Efficiently isolate cells expressing epidermal growth factor receptor (EGFR) from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs), leukapheresis products, or cell culture samples in as little as 15 minutes with the EasySep™ Human EGFR Positive Selection Kit. Desired cells are immediately ready for use in downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Using immunomagnetic EasySep™ technology, EGFR+ cells are labeled with antibodies and magnetic particles and isolated without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off. Depending on your research needs, either fraction may be used downstream.

This kit targets EGFR, which belongs to the ErbB family of tyrosine kinase receptors (RTK) expressed on the cell surface of circulating tumor cells (CTCs), macrophages, monocytes, plasma cells, and certain T cell subsets, such as effector CD4+ T cells and FoxP3-expressing regulatory CD4+ T cells (Tregs) (Sabbah DA et al. Curr Top Med Chem, 2020 and MacDonald F & Zaiss DMW Front Pharmacol, 2017).

Learn more about how immunomagnetic EasySep™ technology works and explore our primary and cultured cells.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
Subtype
Cell Isolation Kits
Cell Type
Brain Tumor Stem Cells, Cancer Cells and Cell Lines, Macrophages, Monocytes, Mononuclear Cells, T Cells, T Cells, CD4+, Tumor Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Brand
EasySep
Area of Interest
Cancer, Cell Therapy Research, Immunology

Data Figures

Figure 1. Typical EasySep™ Human EGFR Positive Selection Kit Isolation Profile

Starting with 10% A549 cells spiked into human PBMCs, the EGFR+ cell content of the isolated fraction is typically 95.0 ± 2.8% (mean ± SD using the purple EasySep™ Magnet). In the above example, the frequencies of the start (left) and final (right) isolated fractions are 10.3% and 95.0%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1131
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-1131
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-1131
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications