EasySep™ Human CD33 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit
Catalog #
17876_C
Immunomagnetic positive selection cell isolation kit
From: 720 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human CD33 Positive Selection Kit II is designed to isolate CD33+ cells from ammonium chloride lysed blood or bone marrow, or from nucleated cells prepared by density separation with RosetteSep™ DM-M density medium (Catalog #15725) by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 antigen is expressed on cells of the myeloid lineage.

This product replaces the EasySep™ Human CD33 Positive Selection Kit (Catalog #18257) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Compatible across EasySep™, "The Big Easy", and RoboSep™-S platforms
Components
  • EasySep™ Human CD33 Positive Selection Kit II (Catalog #17876)
    • EasySep™ Human CD33 Positive Selection Cocktail II, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
  • RoboSep™ Human CD33 Positive Selection Kit II with Filter Tips (Catalog #17876RF)
    • EasySep™ Human CD33 Positive Selection Cocktail II, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Monocytes, Myeloid Cells
Species
Human
Sample Source
PMNC
Selection Method
Positive
Brand
EasySep
Area of Interest
Chimerism, HLA, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human CD33 Positive Selection Kit II
Catalog #
17876
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human CD33 Positive Selection Kit II
Catalog #
17876RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human CD33 Positive Selection Kit II
Catalog #
17876
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human CD33 Positive Selection Kit II
Catalog #
17876
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human CD33 Positive Selection Kit II
Catalog #
17876
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human CD33 Positive Selection Kit II
Catalog #
17876RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human CD33 Positive Selection Kit II
Catalog #
17876RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human CD33 Positive Selection Kit II
Catalog #
17876RF
Lot #
All
Language
English

Educational Materials (11)

Brochure
Cell Separation Products for HLA Analysis
Brochure
EasySep™ Cell Separation Technology
Brochure
HLA Antibodies for Purity Assessment
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with ammonium chloride-lysed peripheral blood, the CD33+ cell content of the isolated fraction is typically 95.6 ± 1.6%, as assessed by labeling with CD14 and CD66b (mean + SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 44.7% and 96.3%, respectively.

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