Control EB or Spheroid Size with AggreWell™

AggreWell™ plates with microwells provide an easy and standardized approach to generate cell aggregates of defined size. Cell aggregates can be generated from many cell types, including cancer cell lines for tumor spheroid formation and human pluripotent stem cells (PSCs) for Embryoid Body (EB)-based differentiation protocols. Different AggreWell™ plate formats are available to suit your particular requirements.



AggreWell™400

AggreWell™800

Microwell size (diameter)

400 µm

800 µm

Aggregate size range (# of cells per microwell)

50 - 3,000

3,000 - 20,000

Plate format

24-well

6 well

24-well

6 well

Number of aggregates generated per well

~ 1,200

~ 7,000

~ 300

~ 1,800

Total number of aggregates per plate

~ 28,000

~ 42,000

~ 7,200

~ 10,800

Catalog #
(1 plate /pack)
(5 plates/pack)

(Starter Kit)*


34411
34415

34450


34421
34425

34460


34811
34815

34850


34821
34825

34860

*Starter Kits contain 2 AggreWell™ plates and 1 bottle of AggreWell™ Rinsing Solution (Catalog #07010). Use of AggreWell™ Rinsing Solution in the plate preparation step is required with the second generation plate.

Here are some helpful tips when using AggreWell™ plates:

  • Prior to using AggreWell™ plates, AggreWell™ Rinsing Solution is required for all cell types during plate preparation steps to ensure optimal performance. AggreWell™ Rinsing Solution prevents cell adhesion and promotes efficient EB and spheroid formation. Add AggreWell™ Rinsing Solution (Catalog #07010) to each well to be used and centrifuge plate at 2000 x g (or at maximum speed) for 5 minutes in a swinging bucket rotor fitted with plate holders.
  • Control just the EB or spheroid size by adjusting the input cell seeding density.
  • Use the right kind of plates for generating the appropriate size EBs. Do not overload the wells in an attempt to generate larger EBs, because overloading will result in the excess cells spilling over the microwell edges, leading to poor performance.
  • When removing large EBs (2000 cells/EB or more) from the wells, use a large bore pipette tip or cut the end of a 1 mL pipette tip with sterile scissors to make the bore larger. This will prevent breaking up formed EBs.
  • AggreWell™ plates are intended for one time use only. Regardless of whether all the wells are used or just one well is used, we do not recommend the plate to be used again for subsequent experiments, so as to prevent contamination.


For more information, please contact Product and Scientific Support at techsupport@stemcell.com.