ClonaCell™ EasyPick

Robotic instrument for automated colony picking and liquid handling

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ClonaCell™ EasyPick

Robotic instrument for automated colony picking and liquid handling

1 Unit
Catalog #30000

Overview

The ClonaCell™ EasyPick is a platform that merges a method for automated selection and cloning of transfected cell lines and hybridomas using our semi-sold media. Unlike other systems, ClonaCell™ EasyPick offers flexible workstations that incorproate multi-handling for upstream and downstream applications, software that selects colonies based on multiple user-specified parameters, disposable tips to eliminate the risk of cross-contamination. With a high throughput of approximately 200 clones per hour, it is the world’s fastest system for automated mammalian colony isolation for industrial-scale development of mammalian cell lines. For more information about Instrument Services including additional service packages please see our instrumentation overview.
Advantages:
• Speed: Generate stable cell lines in only 14 days by combining the selection and cloning steps using ClonaCell™ media
• Efficiency: Isolate up to 200 clones per hour with this fully automated platform
• Free from cross-contamination: Disposable tips, CO-RE pipetting technology, monitored air displacement, and anti-droplet control eliminate the risk of sample cross-contamination or aerosol formation
• Scalable and flexible: Designed with a modular platform to accommodate automation of upstream and downstream processes
Application:
Antibody Development; Cell Line Development; Hybridoma Generation
Brand:
EasyPick
Area of Interest:
Cell Line Development; Drug Discovery and Toxicity Testing; Hybridoma Generation; Semi-Solid Cloning

Technical Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications

(4)
Protein Engineering Design and Selection 2015 December

Optimisation of a system for the co-translational incorporation of a keto amino acid and its application to a tumour-specific Anticalin

Reichert A et al.

Abstract

The bioorthogonal keto group has attracted interest for the site-specific chemical conjugation of recombinant proteins under mild conditions, e.g. with aminooxy-functionalised fluorescent probes, radiometal chelates, toxins or polymers. However, the cotranslational incorporation of the corresponding non-canonical amino acid p-acetyl-L-phenylalanine (Apa) into proteins expressed in Escherichia coli by means of amber suppression using a previously described system with a mutated tRNA and an engineered tyrosyl-tRNA synthetase from Methanococcus jannaschii shows limited efficiency and considerable promiscuity towards endogenous amino acids. Employing a one-plasmid system that encodes all three components required for selection, i.e. the modified aminoacyl-tRNA synthetase (aaRS), the cognate amber suppressor tRNA and the enhanced green fluorescent protein equipped with an amber stop codon and serving as reporter, we have generated an Apa-specific aaRS{\&}tRNA pair with considerably improved efficiency (17-fold increased expression) and also fidelity (6-fold). To this end, both the aaRS and the tRNA were subjected to doped random mutagenesis and selection in altogether four evolutionary cycles using fluorescence-activated bacterial cell sorting as well as automated screening of microcultures. The resulting aaRS{\&}tRNA pair was applied to the functionalisation of an Anticalin with specificity towards oncofetal fibronectin by introducing a keto group at a permissible site for subsequent conjugation with a fluorescent dye, thus allowing visualisation of this tumour target under the microscope.
2013 December

Cell senescence as both a dynamic and a static phenotype

Young A et al.

Abstract

It has been 50 years since cellular senescence was first described in human diploid fibroblasts (HDFs), yet its mechanism as well as its physiological and clinical implications are still not fully appreciated. Recent progress suggests that cellular senescence is a collective phenotype, composed of complex networks of effector programs. The balance and quality within the effector network varies depending on the cell type, the nature of the stress as well as the context. Therefore, understanding each of these effectors in the context of the whole network will be necessary in order to fully understand senescence as a whole. Furthermore, searching for new effector programs of senescence will help to define this heterogeneous and complex phenotype according to cellular contexts.
Journal of Visualized Experiments 2011 September

A high-throughput automated platform for the development of manufacturing cell lines for protein therapeutics

Shi S et al.

Abstract

The fast-growing biopharmaceutical industry demands speedy development of highly efficient and reliable production systems to meet the increasing requirement for drug supplies. The generation of production cell lines has traditionally involved manual operations that are labor-intensive, low-throughput and vulnerable to human errors. We report here an integrated high-throughput and automated platform for development of manufacturing cell lines for the production of protein therapeutics. The combination of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has enabled a high-throughput and more efficient cell line development process. In this operation, production host cells are first transfected with an expression vector carrying the gene of interest (1), followed by the treatment with a selection agent. The stably-transfected cells are then stained with fluorescence-labeled anti-human IgG antibody, and are subsequently subject to flow cytometry analysis (2-4). Highly productive cells are selected based on fluorescence intensity and are isolated by single-cell sorting on a BD FACSAria. Colony formation from single-cell stage was detected microscopically and a series of time-laps digital images are taken by CloneSelect Imager for the documentation of cell line history. After single clones have formed, these clones were screened for productivity by ELISA performed on a TECAN Freedom EVO liquid handling system. Approximately 2,000 - 10,000 clones can be screened per operation cycle with the current system setup. This integrated approach has been used to generate high producing Chinese hamster ovary (CHO) cell lines for the production of therapeutic monoclonal antibody (mAb) as well as their fusion proteins. With the aid of different types of detecting probes, the method can be used for developing other protein therapeutics or be applied to other production host systems. Comparing to the traditional manual procedure, this automated platform demonstrated advantages of significantly increased capacity, ensured clonality, traceability in cell line history with electronic documentation and much reduced opportunity in operator error.
Nature Methods 2006 October

Automatic for the people

Eisenstein M

Abstract

More and more scientists now see advantages in automating some of their more repetitive or error-prone tasks. Michael Eisenstein takes a look at systems that are helping to bring robotics into the academic and clinical research laboratory.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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