Showing 1 - 1 of 1 results for "15086"
- ReferenceLorzadeh A et al. (NOV 2016) Cell reports 17 8 2112--2124
Nucleosome Density ChIP-Seq Identifies Distinct Chromatin Modification Signatures Associated with MNase Accessibility.
Nucleosome position, density, and post-translational modification are widely accepted components of mechanisms regulating DNA transcription but still incompletely understood. We present a modified native ChIP-seq method combined with an analytical framework that allows MNase accessibility to be integrated with histone modification profiles. Application of this methodology to the primitive (CD34+) subset of normal human cord blood cells enabled genomic regions enriched in one versus two nucleosomes marked by histone 3 lysine 4 trimethylation (H3K4me3) and/or histone 3 lysine 27 trimethylation (H3K27me3) to be associated with their transcriptional and DNA methylation states. From this analysis, we defined four classes of promoter-specific profiles and demonstrated that a majority of bivalent marked promoters are heterogeneously marked at a single-cell level in this primitive cell type. Interestingly, extension of this approach to human embryonic stem cells revealed an altered relationship between chromatin modification state and nucleosome content at promoters, suggesting developmental stage-specific organization of histone methylation states. View PublicationCatalog #: Product Name: 05872 ReLeSR™ 15086 Complete Kit for Human Whole Blood CD34+ Cells 85850 mTeSR™1 Catalog #: 05872 Product Name: ReLeSR™ Catalog #: 15086 Product Name: Complete Kit for Human Whole Blood CD34+ Cells Catalog #: 85850 Product Name: mTeSR™1
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