You searched for: 17856
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ReferenceM. Ortiz-Virumbrales et al. (dec 2017) Acta neuropathologica communications 5 1 77
CRISPR/Cas9-Correctable mutation-related molecular and physiological phenotypes in iPSC-derived Alzheimer's PSEN2 N141I neurons.
Basal forebrain cholinergic neurons (BFCNs) are believed to be one of the first cell types to be affected in all forms of AD, and their dysfunction is clinically correlated with impaired short-term memory formation and retrieval. We present an optimized in vitro protocol to generate human BFCNs from iPSCs, using cell lines from presenilin 2 (PSEN2) mutation carriers and controls. As expected, cell lines harboring the PSEN2 N141I mutation displayed an increase in the A$\beta$42/40 in iPSC-derived BFCNs. Neurons derived from PSEN2 N141I lines generated fewer maximum number of spikes in response to a square depolarizing current injection. The height of the first action potential at rheobase current injection was also significantly decreased in PSEN2 N141I BFCNs. CRISPR/Cas9 correction of the PSEN2 point mutation abolished the electrophysiological deficit, restoring both the maximal number of spikes and spike height to the levels recorded in controls. Increased A$\beta$42/40 was also normalized following CRISPR/Cas-mediated correction of the PSEN2 N141I mutation. The genome editing data confirms the robust consistency of mutation-related changes in A$\beta$42/40 ratio while also showing a PSEN2-mutation-related alteration in electrophysiology. View PublicationCatalog #:Product Name:17854EasySep™ Human CD19 Positive Selection Kit II17858EasySep™ Human CD14 Positive Selection Kit II17952EasySep™ Human CD4+ T Cell Isolation Kit05790BrainPhys™ Neuronal Medium17754EasySep™ Release Human CD19 Positive Selection Kit85850mTeSR™117861EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit17877EasySep™ Human CD138 Positive Selection Kit II17856EasySep™ Human CD34 Positive Selection Kit II -
ReferenceM. Labuhn et al. (aug 2019) Cancer cell 36 2 123--138.e10
Mechanisms of Progression of Myeloid Preleukemia to Transformed Myeloid Leukemia in Children with Down Syndrome.
Myeloid leukemia in Down syndrome (ML-DS) clonally evolves from transient abnormal myelopoiesis (TAM), a preleukemic condition in DS newborns. To define mechanisms of leukemic transformation, we combined exome and targeted resequencing of 111 TAM and 141 ML-DS samples with functional analyses. TAM requires trisomy 21 and truncating mutations in GATA1; additional TAM variants are usually not pathogenic. By contrast, in ML-DS, clonal and subclonal variants are functionally required. We identified a recurrent and oncogenic hotspot gain-of-function mutation in myeloid cytokine receptor CSF2RB. By a multiplex CRISPR/Cas9 screen in an in vivo murine TAM model, we tested loss-of-function of 22 recurrently mutated ML-DS genes. Loss of 18 different genes produced leukemias that phenotypically, genetically, and transcriptionally mirrored ML-DS. View PublicationCatalog #:Product Name:09600StemSpan™ SFEM19860EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit17856EasySep™ Human CD34 Positive Selection Kit II -
ReferenceE. Drent et al. (jul 2019) Clinical cancer research : an official journal of the American Association for Cancer Research 25 13 4014--4025
Combined CD28 and 4-1BB Costimulation Potentiates Affinity-tuned Chimeric Antigen Receptor-engineered T Cells.
PURPOSE Targeting nonspecific, tumor-associated antigens (TAA) with chimeric antigen receptors (CAR) requires specific attention to restrict possible detrimental on-target/off-tumor effects. A reduced affinity may direct CAR-engineered T (CAR-T) cells to tumor cells expressing high TAA levels while sparing low expressing normal tissues. However, decreasing the affinity of the CAR-target binding may compromise the overall antitumor effects. Here, we demonstrate the prime importance of the type of intracellular signaling on the function of low-affinity CAR-T cells. EXPERIMENTAL DESIGN We used a series of single-chain variable fragments (scFv) with five different affinities targeting the same epitope of the multiple myeloma-associated CD38 antigen. The scFvs were incorporated in three different CAR costimulation designs and we evaluated the antitumor functionality and off-tumor toxicity of the generated CAR-T cells in vitro and in vivo. RESULTS We show that the inferior cytotoxicity and cytokine secretion mediated by CD38 CARs of very low-affinity (Kd {\textless} 1.9 × 10-6 mol/L) bearing a 4-1BB intracellular domain can be significantly improved when a CD28 costimulatory domain is used. Additional 4-1BB signaling mediated by the coexpression of 4-1BBL provided the CD28-based CD38 CAR-T cells with superior proliferative capacity, preservation of a central memory phenotype, and significantly improved in vivo antitumor function, while preserving their ability to discriminate target antigen density. CONCLUSIONS A combinatorial costimulatory design allows the use of very low-affinity binding domains (Kd {\textless} 1 mumol/L) for the construction of safe but also optimally effective CAR-T cells. Thus, very-low-affinity scFvs empowered by selected costimulatory elements can enhance the clinical potential of TAA-targeting CARs. View PublicationCatalog #:Product Name:04534MethoCult™ H4534 Classic Without EPO17856EasySep™ Human CD34 Positive Selection Kit II -
ReferenceM. Kaur et al. (feb 2019) Journal of immunology (Baltimore, Md. : 1950)
Induction and Therapeutic Targeting of Human NPM1c+ Myeloid Leukemia in the Presence of Autologous Immune System in Mice.
Development of targeted cancer therapy requires a thorough understanding of mechanisms of tumorigenesis as well as mechanisms of action of therapeutics. This is challenging because by the time patients are diagnosed with cancer, early events of tumorigenesis have already taken place. Similarly, development of cancer immunotherapies is hampered by a lack of appropriate small animal models with autologous human tumor and immune system. In this article, we report the development of a mouse model of human acute myeloid leukemia (AML) with autologous immune system for studying early events of human leukemogenesis and testing the efficacy of immunotherapeutics. To develop such a model, human hematopoietic stem/progenitor cells (HSPC) are transduced with lentiviruses expressing a mutated form of nucleophosmin (NPM1), referred to as NPM1c. Following engraftment into immunodeficient mice, transduced HSPCs give rise to human myeloid leukemia, whereas untransduced HSPCs give rise to human immune cells in the same mice. The de novo AML, with CD123+ leukemic stem or initiating cells (LSC), resembles NPM1c+ AML from patients. Transcriptional analysis of LSC and leukemic cells confirms similarity of the de novo leukemia generated in mice with patient leukemia and suggests Myc as a co-operating factor in NPM1c-driven leukemogenesis. We show that a bispecific conjugate that binds both CD3 and CD123 eliminates CD123+ LSCs in a T cell-dependent manner both in vivo and in vitro. These results demonstrate the utility of the NPM1c+ AML model with an autologous immune system for studying early events of human leukemogenesis and for evaluating efficacy and mechanism of immunotherapeutics. View PublicationCatalog #:Product Name:17856EasySep™ Human CD34 Positive Selection Kit II -
Product Information Sheet (PIS)
10000000695-PIS_01.pdf
Catalog #LotLanguageProduct Name:17856AllEnglishEasySep™ Human CD34 Positive Selection Kit II17856RFAllEnglishEasySep™ Human CD34 Positive Selection Kit II -
Safety Data Sheet (SDS)
DX22395-SDS_1_0_0.pdf
Catalog #Product Name:17851EasySep™ Human CD3 Positive Selection Kit II17851RFRoboSep™ Human CD3 Positive Selection Kit II17852EasySep™ Human CD4 Positive Selection Kit II17852RFRoboSep™ Human CD4 Positive Selection Kit II17853EasySep™ Human CD8 Positive Selection Kit II17853RFRoboSep™ Human CD8 Positive Selection Kit II17854EasySep™ Human CD19 Positive Selection Kit II17854RFRoboSep™ Human CD19 Positive Selection Kit II17855EasySep™ Human CD56 Positive Selection Kit II17855RFRoboSep™ Human CD56 Positive Selection Kit II17858EasySep™ Human CD14 Positive Selection Kit II17858RFRoboSep™ Human CD14 Positive Selection Kit II17896EasySep™ Human Cord Blood CD34 Positive Selection Kit II17896RFRoboSep™ Human CB CD34 Positive Selection Kit II18063EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit18765EasySep™ Mouse CD4+CD62L+ T Cell Isolation Kit18765RFRoboSep™ Mouse CD4+CD62L+ T Cell Isolation Kit18780RFRoboSep™ Mouse CD11c Positive Selection Kit II18782EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit18782RFRoboSep™ Mouse CD25 Regulatory T cell Positive Selection Kit18951EasySep™ Mouse CD90.2 Positive Selection Kit II18952EasySep™ Mouse CD4 Positive Selection Kit II18952RFRoboSep™ Mouse CD4 Positive Selection Kit II18953EasySep™ Mouse CD8a Positive Selection Kit II18953RFRoboSep™ Mouse CD8a Positive Selection Kit II18954EasySep™ Mouse CD19 Positive Selection Kit II15086Complete Kit for Human Whole Blood CD34+ Cells15086RFRoboSep™ Complete Kit for Human Whole Blood CD34+ Cells05925CD34+ Progenitor Reprogramming Kit17897EasySep™ Human Cord Blood CD34 Positive Selection Kit III17897RFRoboSep™ Human Cord Blood CD34 Positive Selection Kit III18780EasySep™ Mouse CD11c Positive Selection Kit II18781EasySep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium18970EasySep™ Mouse CD11b Positive Selection Kit II18970RFRoboSep™ Mouse CD11b Positive Selection Kit II18781RFRoboSep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium18783EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II18957EasySep™ Mouse CD138 Positive Selection Kit18958EasySep™ Mouse CD90.1 Positive Selection Kit18990EasySep™ Anti-Rat IgG2a Positive Selection Kit18992EasySep™ Anti-Rat IgG2b Positive Selection Kit18994EasySep™ Anti-Rat IgM Positive Selection Kit18783RFRoboSep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II18957RFRoboSep™ Mouse CD138 Positive Selection Kit18958RFRoboSep™ Mouse CD90.1 Positive Selection Kit18990RFRoboSep™ Anti-Rat IgG2a Positive Selection Kit18992RFRoboSep™ Anti-Rat IgG2b Positive Selection Kit18994RFRoboSep™ Anti-Rat IgM Positive Selection Kit18651EasySep™ Human CD15 Positive Selection Kit17887EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II17887RFRoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II17878EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit17878RFRoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit17663EasySep™ Human Biotin Positive Selection Kit II17663RFRoboSep™ Human Biotin Positive Selection Kit II17664EasySep™ Human PE Positive Selection Kit II17664RFRoboSep™ Human PE Positive Selection Kit II17684EasySep™ PE Positive Selection Kit II17684RFRoboSep™ PE Positive Selection Kit II17698EasySep™ "Do-It-Yourself" Positive Selection Kit II17698RFRoboSep™ "Do-It-Yourself" Positive Selection Kit II17699EasySep™ Human "Do-It-Yourself" Positive Selection Kit II17699RFRoboSep™ Human "Do-It-Yourself" Positive Selection Kit II17661EasySep™ Human APC Positive Selection Kit II17661RFRoboSep™ Human APC Positive Selection Kit II17662EasySep™ Human FITC Positive Selection Kit II17662RFRoboSep™ Human FITC Positive Selection Kit II17665EasySep™ Mouse Biotin Positive Selection Kit II17665RFRoboSep™ Mouse Biotin Positive Selection Kit II17666EasySep™ Mouse PE Positive Selection Kit II17666RFRoboSep™ Mouse PE Positive Selection Kit II17667EasySep™ Mouse APC Positive Selection Kit II17667RFRoboSep™ Mouse APC Positive Selection Kit II17668EasySep™ Mouse FITC Positive Selection Kit II17668RFRoboSep™ Mouse FITC Positive Selection Kit II17681EasySep™ APC Positive Selection Kit II17681RFRoboSep™ APC Positive Selection Kit II17682EasySep™ FITC Positive Selection Kit II17682RFRoboSep™ FITC Positive Selection Kit II17683EasySep™ Biotin Positive Selection Kit II17683RFRoboSep™ Biotin Positive Selection Kit II17888EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit17888RFRoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit17846EasySep™ Human EpCAM Positive Selection Kit II17846RFRoboSep™ Human EpCAM Positive Selection Kit II17849EasySep™ Human CD271 Positive Selection Kit II17856EasySep™ Human CD34 Positive Selection Kit II17856RFEasySep™ Human CD34 Positive Selection Kit II17879EasySep™ Human Whole Blood CD34 Positive Selection Kit II17879RFRoboSep™ Human Whole Blood CD34 Positive Selection Kit II17893EasySep™ Human Myeloid Positive Selection Kit II17893RFRoboSep™ Human Myeloid Positive Selection Kit II17965EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit18945EasySep™ Mouse CD45 Positive Selection Kit100-0350EasySep™ Mouse TIL (CD45) Positive Selection Kit -
26:26WebinarOnline Immunology Journal Club: Evaluating Functional Immune Responses in the Next Generation of Humanized Mice
Publish Date: August 10, 2020 Views: 0 -
BrochureHematopoietic Stem and Progenitor Cells - Products for Your Research
Cell Culture Media and SupplementsErythroClear, MethoCult, MyeloCult, SmartDish, StemSpan, STEMvision -
1:13VideoIsolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Publish Date: October 02, 2009 Views: 0 -
1:57VideoHow EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Publish Date: April 20, 2016 Views: 0 -
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1:37VideoHow to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Publish Date: March 13, 2012 Views: 0 -
BrochureCell Separation Products for HLA Analysis
Cell Isolation ProductsEasySep, RoboSep, RosetteSep -
WallchartFrequencies of Cell Types in Human Peripheral Blood
List of the frequencies of cell types in human peripheral blood