3D Cancer Spheroids
Making Your Research Better
The human body is a 3-dimensional system, yet most cell culture research uses 2-dimensional monolayers. 2D cultures do not accurately reflect cell attachment, cytoskeletal structure, or the in vivo behavior of cells. 3D cultures are more physiologically relevant, providing a better representation of disease state and drug response.
3D Cancer Spheroids Mimic In Vivo Tumors
- Tumorigenesis, spheroid morphology, self-organization, cytoskeletal structure, signalling, cancer progression, and more.
- Gradients of oxygen and nutrient availability throughout the tumor, roles of outer vs inner cells of the tumor.
- 3D cell-cell and cell-ECM interactions, roles of stromal cells and cancer-associated fibroblasts, co-cultures.
- Epithelial-mesencyhmal transition, migration, and ultimate survival of cancer in secondary sites.
- Model solid tumor response to drug treatment, predict drug response, drug kinetics, cytotoxicity, and diffusion.
- Model cytotoxic effects of T cells or NK cells using a 3D tumor model.
AggreWell™ Plates for 3D Cultures: Improve Your Reproducibility
AggreWell™ helps ensure uniformity of spheroids and consistency from experiment to experiment.
AggreWell™ plates with microwells provide an easy way to generate large numbers of multicellular spheroids. Unlike traditional spheroid-formation methods, spheroids generated in AggreWell™ are highly uniform in size and shape, and the size can be easily controlled by modifying the input cell seeding density. With added size-control and uniformity, AggreWell improves reproducibility of your 3D research.
Why use AggreWell™?
- Generate large numbers of spheroids from a single pipetting step compared to at least 8 pipetting steps with a 96-or 384-well ULA or hanging drop plate. Fewer processing steps means fewer opportunities for error.
- Spheroids generated in AggreWell™ are highly uniform in size and shape, meaning that your starting population is consistent within and between experiments. Get true technical replicates, without compounding population or processing effects.
- Large numbers of spheroids facilitate larger, more comprehensive studies with statistically significant sample sizes. A statistically significant sample size increases the scientific accuracy and producibility of your experimental results.
AggreWell™ helps you ensure the clonality of your sphere-forming assay.
In the sphere-forming assay, spheres are generated from a single cell, often referred to as a Tumor-Initiating Cell (TIC) or a Cancer Stem-like Cell (CSC), which is capable of self-renewal and proliferation. The sphere-forming assay is a common method used to determine the frequency of the TICs in a given cancer cell population. However, achieving clonality requires limiting dilution, which can be difficult and laborious. By using AggreWell™ with microwells, clonal spheres are easy to achieve without the need for limiting dilution.
Why use AggreWell™?
- With 1,200 microwells in each well (AggreWell™400 24-well), there are 1,200 opportunities for each cell to settle into a microwell and be physically sequestered away from other cells, preventing migration and aggregation.
- Get clonal results by seeding at limiting dilution densities in a single pipetting step.
- At limiting dilution cell density and the ability to visually confirm single cells in microwells, the accuracy of your clonal sphere-forming assay can be verified.
Learn More or Try AggreWell™ in your Lab
Explore These Resources
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- Edmondson R et al. (2014) Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors. Assay Drug Dev Technol 12(4): 207-18.
- Wrzesinski K et al. (2014) The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments. PLoS ONe 9(9); e1069.
- Hirschhaeuser F et al. (2010) Multicellular tumor spheroids: an underestimated tool is catching up again. Biotechnol 148(1): 3-1.
- Gaskell H et al. (2016) Characterization of a functional C3A liver spheroid model. Toxicol Res (Camb) 5(4): 1053-65.
- Wenzel C et al. (2014) D high-content screening for the identification of compounds that target cells in dormant tumor spheroid regions. Exp Cell Res 323(1): 131-43.
- Song Y et al. (2016) Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer. PNAS 108(15): 6235-40.
- Kondo J et al. (2011) Activated hepatic stellate cells play pivotal roles in hepatocellular carcinoma cell chemoresistance and migration in multicellular tumor spheroids. Sci Rep 6: 63750.
- Metzger W et al. (2014) Evaluation of cell-surface interaction using a 3D spheroid cell culture model on artificial extracellular matrices. Mater Sci Eng C Biol Appl 1(73): 310-18.
- Vinci M et al. (2012) Advances in establishment & analysis of three-dimensional tumor spheroid-based functional assays for target validation & drug evaluation. BMC Biol 10:29 DOI: 10.1186/1741-7007-10-29.
- Pauli C et al. (2017) Personalized In Vitro and In Vivo Cancer Models to Guide Precision Medicine. Cancer Dis Epub doi: 10.1158/2159-8290.
- Fey S et al. (2012) Determination of Drug Toxicity Using 3D Spheroids Constructed From an Immortal Human Hepatocyte Cell Line. Toxicol Sci 127(2): 403-11.
- Sarisozen C et al. (2014) The effect of co-delivery of paclitaxel and curcumin by transferrin-targeted PEG-PE-based mixed micelles on resistant ovarian cancer in 3-D spheroids and in vivo tumors. Eur J Pharm Biopharm 88(2): 539-50.
- Barrera-Rodríguez R et al. (2012) Multidrug resistance characterization in multicellular tumour spheroids from two human lung cancer cell lines. Cancer Cell Int 10: 47.
- Sirenko O et al. (2016) Phenotypic Characterization of Toxic Compound Effects on Liver Spheroids Derived from iPSC Using Confocal Imaging and Three-Dimensional Image Analysis. Assay Drug Dev Technol 14(7): 381-94.
- Giannattasio A et al. (2015) Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids. BMC Cancer 15:351 DOI:10.1186/s12885-015-1321-y.