3D Cancer Spheroids

Making Your Research Better

The human body is a 3-dimensional system, yet most cell culture research uses 2-dimensional monolayers. 2D cultures do not accurately reflect cell attachment, cytoskeletal structure, or the in vivo behavior of cells. 3D cultures are more physiologically relevant, providing a better representation of disease state and drug response.

3D Cancer Spheroids Mimic In Vivo Tumors

  • Tumorigenesis, spheroid morphology, self-organization, cytoskeletal structure, signalling, cancer progression, and more.
  • Gradients of oxygen and nutrient availability throughout the tumor, roles of outer vs inner cells of the tumor.
  • 3D cell-cell and cell-ECM interactions, roles of stromal cells and cancer-associated fibroblasts, co-cultures.
  • Epithelial-mesencyhmal transition, migration, and ultimate survival of cancer in secondary sites.
  • Model solid tumor response to drug treatment, predict drug response, drug kinetics, cytotoxicity, and diffusion.
  • Model cytotoxic effects of T cells or NK cells using a 3D tumor model.

AggreWell™ Plates for 3D Cultures: Improve Your Reproducibility

AggreWell™ helps ensure uniformity of spheroids and consistency from experiment to experiment.

AggreWell™ plates with microwells provide an easy way to generate large numbers of multicellular spheroids. Unlike traditional spheroid-formation methods, spheroids generated in AggreWell™ are highly uniform in size and shape, and the size can be easily controlled by modifying the input cell seeding density. With added size-control and uniformity, AggreWell improves reproducibility of your 3D research.

Why use AggreWell™?

  • Generate large numbers of spheroids from a single pipetting step compared to at least 8 pipetting steps with a 96-or 384-well ULA or hanging drop plate. Fewer processing steps means fewer opportunities for error.
  • Spheroids generated in AggreWell™ are highly uniform in size and shape, meaning that your starting population is consistent within and between experiments. Get true technical replicates, without compounding population or processing effects.
  • Large numbers of spheroids facilitate larger, more comprehensive studies with statistically significant sample sizes. A statistically significant sample size increases the scientific accuracy and producibility of your experimental results.

AggreWell™ helps you ensure the clonality of your sphere-forming assay.

In the sphere-forming assay, spheres are generated from a single cell, often referred to as a Tumor-Initiating Cell (TIC) or a Cancer Stem-like Cell (CSC), which is capable of self-renewal and proliferation. The sphere-forming assay is a common method used to determine the frequency of the TICs in a given cancer cell population. However, achieving clonality requires limiting dilution, which can be difficult and laborious. By using AggreWell™ with microwells, clonal spheres are easy to achieve without the need for limiting dilution.

Why use AggreWell™?

  • With 1,200 microwells in each well (AggreWell™400 24-well), there are 1,200 opportunities for each cell to settle into a microwell and be physically sequestered away from other cells, preventing migration and aggregation.
  • Get clonal results by seeding at limiting dilution densities in a single pipetting step.
  • At limiting dilution cell density and the ability to visually confirm single cells in microwells, the accuracy of your clonal sphere-forming assay can be verified.

Learn More or Try AggreWell™ in your Lab


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