This rabbit monoclonal antibody (clone 249) reacts with synaptophysin, a 38 kDa integral transmembrane protein possessing four transmembrane domains and a major small synaptic synaptic vesicle protein. Use this recombinant monoclonal antibody in applications requiring detection of synaptophysin, such as immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF). This antibody clone has been verified for labeling synaptophysin-positive puncta in hPSC-derived forebrain-type neurons generated with STEMdiff™ Forebrain Neuron Differentiation Kit (Catalog #08600) and STEMdiff™ Forebrain Neuron Maturation Kit (Catalog #08605).

It interacts with a variety of different motor proteins and synaptic vesicles including dynamin I, myosin V, vesicular proton pump V-ATPase, and adaptor protein 1 (AP-1). Synaptophysin is extensively involved in vesicular functions such as protein sorting and priming, as well as synapse formation, exocytosis, and endocytosis. Synaptophysin is also a potent marker of axonal damage; synaptophysin was discovered colocalized with amyloid precursor protein (APP), a marker of axonal damage in patients with multiple sclerosis. Synaptophysin deletion in knock-out mice have been correlated with reduced spatial learning and impaired object novelty recognition.