Anti-Human CD25 Antibody, Clone BC96

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD25

Anti-Human CD25 Antibody, Clone BC96

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD25

From: 65 USD
Catalog #
(Select a product)
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD25
Add to Wish List

Overview

The BC96 antibody reacts with human CD25, a 55 kDa type I integral transmembrane glycoprotein. CD25 is involved in the activation and proliferation of lymphocytes and can be used in the identification of T cell subsets in viral infections like HIV and HTLV. CD25 is present on activated T and B cells, monocytes, macrophages, and regulatory T cells. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for regulatory T cells. CD25 has a low affinity for its IL-2 ligand but associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2 receptor.
Subtype
Primary Antibodies
Target Antigen
CD25
Alternative Names
IL-2 Receptor alpha chain, IL-2Ra, IL2Ra, Tac, p55
Reactive Species
Baboon, Chimpanzee, Cynomolgus, Human, Macaque, Rhesus
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
B Cells, T Cells, T Cells, Regulatory
Application
Flow Cytometry
Area of Interest
Cancer, Immunology, Transplantation Research, Cell Therapy Development
Clone
BC96
Gene ID
3559
Isotype
IgG1, kappa

Data Figures

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, followed by a rat anti-mouse IgG1 antibody, FITC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, FITC (solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, FITC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE; solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ; solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, Alexa Fluor® 488 (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD; solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60158AZ.1, 60158AZ
Lot #
All
Language
English
Catalog #
60158AD.1, 60158AD
Lot #
All
Language
English
Catalog #
60158BT
Lot #
All
Language
English
Catalog #
60158PE, 60158PE.1
Lot #
All
Language
English
Catalog #
60158FI.1, 60158FI
Lot #
All
Language
English
Catalog #
60158
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60158AZ.1, 60158AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60158AD.1, 60158AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60158BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60158PE, 60158PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60158FI.1, 60158FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60158
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications