This mouse monoclonal antibody (clone 5C8, APC-conjugated) reacts with human CD154 (CD40L), a member of the TNF-α superfamily with a molecular weight of 39 kDa. CD154, also known as CD40L, gp39, TRAP, and T-BAM, is a type II transmembrane glycoprotein that is predominantly expressed on activated T-cells, but is also found in NK cells, mast cells, basophils, and eosinophils. CD154 (CD40L) binds to CD40, a costimulatory protein found on antigen-presenting cells, such as B cells, monocytes, dendritic cells, and fibroblasts. The CD154 (CD40)-CD40L interaction induces the activation and proliferation of B-cells, allowing for antibody production, isotype switching, and B-cell memory development. Use this monoclonal antibody in applications requiring detection of CD154/CD40L such as flow cytometry (FC).

CD154 (CD40L) expression is upregulated in inflammatory bowel disease, suggesting its role in regulating pathogenic cytokine production in T-cells. In vitro studies also demonstrate that the binding of CD40 to its ligand induces endothelial cell activation in vitro, highlighting the immunopathogenic role that CD40 and its ligand plays. The CD154 (CD40L), clone 5C8 antibody has been reported to block the interaction and ligation of CD40 with its ligand.