Anti-Human CD154 (CD40L) Antibody, Clone 5C8

Mouse monoclonal antibody against human CD154 (CD40L)

Anti-Human CD154 (CD40L) Antibody, Clone 5C8

Mouse monoclonal antibody against human CD154 (CD40L)

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Mouse monoclonal antibody against human CD154 (CD40L)
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Overview

This mouse monoclonal antibody (clone 5C8, APC-conjugated) reacts with human CD154 (CD40L), a member of the TNF-α superfamily with a molecular weight of 39 kDa. CD154, also known as CD40L, gp39, TRAP, and T-BAM, is a type II transmembrane glycoprotein that is predominantly expressed on activated T-cells, but is also found in NK cells, mast cells, basophils, and eosinophils. CD154 (CD40L) binds to CD40, a costimulatory protein found on antigen-presenting cells, such as B cells, monocytes, dendritic cells, and fibroblasts. The CD154 (CD40)-CD40L interaction induces the activation and proliferation of B-cells, allowing for antibody production, isotype switching, and B-cell memory development. Use this monoclonal antibody in applications requiring detection of CD154/CD40L such as flow cytometry (FC).

CD154 (CD40L) expression is upregulated in inflammatory bowel disease, suggesting its role in regulating pathogenic cytokine production in T-cells. In vitro studies also demonstrate that the binding of CD40 to its ligand induces endothelial cell activation in vitro, highlighting the immunopathogenic role that CD40 and its ligand plays. The CD154 (CD40L), clone 5C8 antibody has been reported to block the interaction and ligation of CD40 with its ligand.
Subtype
Primary Antibodies
Target Antigen
CD154
Alternative Names
CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Reactive Species
Human
Conjugation
APC-Cyanine7, PE, Unconjugated
Host Species
Mouse
Cell Type
T Cells
Species
Human
Application
Flow Cytometry
Area of Interest
Immunology, Stem Cell Biology
Clone
5C8
Gene ID
959
Isotype
IgG2a, kappa

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1350
Lot #
All
Language
English
Catalog #
100-1352
Lot #
All
Language
English
Catalog #
100-1351
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1350
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1352
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1351
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications