Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3

Mouse monoclonal IgG2a antibody against human, mouse, rat beta­tubulin III

More Views

From: 125 USD

Options

* Required Fields

Catalog # (Select a product)
Mouse monoclonal IgG2a antibody against human, mouse, rat beta­tubulin III
From: 125 USD

Overview

The 2G10-TB3 antibody reacts with beta-tubulin III, an ~50 - 55 kDa structural protein that is a constituent of tubulin. Tubulin is the major component of microtubules within the cytoskeleton and is assembled from heterodimers of alpha- and beta-tubulin subunits. The beta III isoform of tubulin, also known as neuron-specific class III beta-tubulin, is expressed primarily in neurons and is widely used as a marker to distinguish neurons from other cell types. Beta-tubulin III contributes to microtubule formation in neuronal cell bodies and axons, a function involving GTP binding, and plays roles in axonal transport, neuronal cell proliferation, and differentiation. It is highly expressed in several types of cancer and is a predictive and prognostic marker for various tumors, for example, being found in neoplastic but not in normal glial cells. The 2G10-TB3 antibody is expected to recognize all mammalian homologs of beta-tubulin III and the epitope has reportedly been mapped to the extreme C-terminal amino acids, EAQGPK.
Subtype:
Primary Antibodies
Target Antigen:
Beta-Tubulin III
Alternative Names:
Class 3 beta-tubulin, class III beta-tubulin, MC1R, neuron-specific class 3 beta-tubulin, neuron-specific class III beta-tubulin, TUBB 3, TUBB3, tubulin beta 3, tubulin beta 4, tubulin beta III
Reactive Species:
Human; Mouse; Rat
Conjugation:
Unconjugated
Host Species:
Mouse
Cell Type:
Neural Cells, PSC-Derived; Neurons
Application:
Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Neuroscience
Clone:
2G10-TB3
Gene ID:
10381
Isotype:
IgG2a, kappa

Scientific Resources

Educational Materials

(3)

Data and Publications

Data

Figure 1. Data for Unconjugated

(A) E18 cortical rat neurons were cultured using NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3, followed by donkey anti-mouse IgG, Alexa Fluor® 488. Nuclei were counterstained with DAPI. Inset shows cells labeled with a mouse IgG2a, kappa isotype control antibody followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counterstained with DAPI. (B) Western blot analysis of denatured/reduced cell lysates with Anti-Beta Tubulin III Antibody, Clone 2G10-TB3. Lane 1, adult rat brain cortical cells; lane 2 (negative control), mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse & Rat).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.