PneumaCult™-ALI Medium

Serum- and BPE-free medium for human airway epithelial cells cultured at the air-liquid interface or as airway organoids

More Views

PneumaCult™-ALI Medium with 12 mm Transwell® Inserts

Medium and Transwell® inserts for human airway epithelial cells cultured at the air-liquid interface

1 Kit
Catalog #05021
466 USD

PneumaCult™-ALI Medium with 6.5 mm Transwell® Inserts

Medium and Transwell® inserts for human airway epithelial cells cultured at the air-liquid interface

1 Kit
Catalog #05022
466 USD

PneumaCult™-ALI Medium

Serum- and BPE-free medium for human airway epithelial cells cultured at the air-liquid interface or as airway organoids

1 Kit
Catalog #05001
197 USD

Overview

PneumaCult™-ALI Medium (Catalog #05001) is a serum- and BPE-free medium for the culture of human airway epithelial cells at the air-liquid interface (ALI). Airway epithelial cells cultured in PneumaCult™-ALI Medium undergo extensive mucociliary differentiation to form a pseudostratified epithelium that exhibits morphological and functional characteristics similar to those of the human airway in vivo. PneumaCult™-ALI Medium is also available in a kit that includes 12 mm Transwell® inserts (Catalog #05021) or 6.5 mm Transwell® inserts (Catalog #05022).

Additionally, PneumaCult™-ALI Medium supports the generation of differentiated airway organoids in a 3D culture system. For a detailed protocol, refer to the Technical Bulletin: A Sphere Culture Method for Mucociliary Differentiation of Primary Human Bronchial Epithelial Cells (Document #28216), available at www.stemcell.com or contact us to request a copy.

Together, PneumaCult™-ALI Medium and PneumaCult™-Ex Plus Medium (Catalog #05040) constitute a fully integrated BPE-free culture system for in vitro human airway modeling. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.