Acquiring DNA or RNA from Positively Selected Cells

Cells positvely selected with the EasySep™ system do not interfere with most downstream applications

Immunomagnetic cell isolation

Cells positively selected with the EasySep™ system do not interfere with most downstream applications. However, in some instances, removing EasySep™ magnetic particles from the positively selected cells before DNA or RNA isolation may improve the DNA/RNA yield.

To remove EasySep™ magnetic particles:

  • Perform cell lysis using a lysis buffer in your DNA/RNA kit.
  • Centrifuge the lysate in a mirocentrifuge tube at maximum speed for 3 minutes. This will pellet cellular debris and magnetic particles, and the lysate will remain in the supernatant.
  • Carefully collect the supernatant while avoiding the pellet.
  • Proceed with the DNA/RNA extraction procedure.

Note: Centrifugation is the preferred method, because the lysate can become sticky after the addition of the lysis buffer.

For more information or assistance, please contact

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.