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STEMCELL Videos
AggreWellTM Introduction
An introduction to a new and innovative way of make uniform, size-controlled embryoid bodies
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AggreWell™400 - webinar
This webinar by Dr. Mark Ungrin describes how AggreWell™ can be used to make uniform embryoid bodies from hESC and hiPSC as well as other cell types.
ALDEFLUOR® - Identify virtually any type of stem and progenitor cells
An innovative, antibody-free system to rapidly identify stem and progenitor cells of several tissue types, including a variety of cancer stem cells. Watch how the ALDEFLUOR® system works. /VD011_Aldefluor_thumbnail.jpg)
ALDEFLUOR® and Cancer Stem Cells Webinar
This webinar presentation explains the principles of ALDEFLUOR® technology, offers literature review and summary of technical considerations of performing and interpreting ALDEFLUOR® assays on primary tissues and tumor cell lines. 
Cell Separation Solutions for HLA and Chimerism Analysis
Learn how to achieve high-volume sample processing for clear, reliable HLA test results with STEMCELL Technologies’ specialized cell isolation products for HLA and Chimerism analysis.
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ClonaCell®: Rapid Cell Line Development
Generate stable cell lines in as little as 14 days by combining selection and cloning using ClonaCell® media.
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ClonaCell® EasyPick: Automated High Throughput Cell Line Generation
STEMCELL Technologies’ automated platform for cell line generation merges the rapid selection and cloning of cells using ClonaCell® media with Hamilton’s 30 years of experience in robotics to meet the three most important aspects of developing cell lines for biologics production: speed, throughput, and quality.
ClonaCell®-HY 96-well Protocol: Selection and Cloning
Learn how to incorporate semi-solid medium into your hybridoma selection and cloning steps to obtain monoclonal hybridomas in 12 days. The ClonaCell®-HY 96-well protocol eliminates medium changes, limiting dilutions and excessive colony picking.
ClonaCell®-HY 96-well protocol: Complete protocol
Optimize monoclonal antibody production with the ClonaCell®-HY 96-Well Protocol. Monoclonal hybridomas can be obtained in 12 days with this protocol to eliminate medium changes, limiting dilutions and excessive colony picking. The ClonaCell®-HY 96-Well Protocol centers around a methylcellulose-based semi-solid medium containing selective agents, growth factors and supplements optimized to support the selection and growth of hybridoma clones in 96-well plates.
Contract Assay Services: Reach Your Milestones Earlier
This video provides an overview of the services and expertise Contract Assay Services offers that may help you expedite the drug discovery process. /video_thumbnail_CAS.jpg)
Contract Assay Services: Hematopoietic, Neural, and Mesenchymal Toxicity Testing
This information session provides an overview of neural, mesenchymal and hematopoietic stem cell biology in the context of the assay systems used to study them and how assays using these primary adult stem cells can be utilized as efficient screening tools in early drug development. Contract Assay Services offers standardized assays and services by combining the expertise of our scientific team with the power of the standardized reagents from STEMCELL Technologies. /VD013_CAS_image.gif)
Contract Assay Services: A Guide to Erythropoiesis
This presentation provides an overview of erythropoiesis and erythropoietin (EPO) biology, mechanisms of EPO-receptor signaling, as well as in vitro and in vivo assays used to determine the functional activity of EPO. /CAS01_Image.gif)
Contract Assay Services: The Utility of Hematopoietic Colony-Forming Cell Assays in Drug Development
This series will describe the utility of in vitro hematopoietic assays at different stages in the drug development process to show how they can be used to examine potential drug targets, saving both time and money. It covers the advantages of hematopoietic in vitro assays for toxicity testing as well as for testing the efficacy of growth factor mimentics. /CAS03_image.gif)
EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
Fast, easy, and gentle. EasySep® is a powerful immunomagnetic cell separation technology that combines the specificity of monoclonal antibodies with the simplicity of a column-free system to isolate highly purified cells. This video describes the EasySep® technology and how to isolate virtually any cell type from any source with EasySep®. 
The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
Watch the video to learn how to perform the EasySep® pour-off.
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Enumerating Mammospheres and Tumorspheres from Cells Cultured in MammoCult® Medium
Mammosphere and tumorsphere cultures are used to evaluate normal and tumor mammary precursor cells in an in vitro system. Accurately estimating the number of hundreds of spheres in your cultures can be a challenge. In this video we demonstrate a highly efficient way to quickly enumerate the spheres and prepare them for your downstream applications. /09005_CV_Mammosphere_Protocol_thumbnail.jpg)
Ficoll-PaqueTM PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
This video provides step-by-step instructions on how to isolate mononuclear cells from whole blood using a density gradient medium such as Ficoll-Paque™ PLUS. Watch the procedure being performed, which includes techniques such as layering diluted blood over Ficoll-Paque™ PLUS and collecting desired cells from the plasma/Ficoll-Paque™ PLUS interface. Ficoll-Paque™ PLUS can be used either prior to downstream cell separations with EasySep® or in conjunction with RosetteSep®. /VD015_thumbnail.jpg)
HetaSepTM: A Guide to Isolating Nucleated Cells from Whole Blood
HetaSep™ is a quick and gentle system for isolating nucleated cells from whole blood. This video provides step-by-step instructions on how to isolate nucleated cells with HetaSep™. Learn how to use HetaSep™ to remove over 95% of erythrocytes from whole blood samples while leaving nucleated cells untouched and ready for downstream EasySep® use. /VD014_thumbnail.jpg)
Isolation of MSCs from Mouse Compact Bone
Mouse MSCs have been reported to occur at higher frequency in compact bone compared to bone marrow. This instructional video provides step-by-step instructions on how to isolate MSCs from mouse compact bone. /09003_mouse_MSC_isolation_thumbnail.jpg)
Mammary Epithelial Cell Colony Interactive Tutorial
The mammary colony forming cell (Ma-CFC) assay is used to quantitate and evaluate mammary progenitor cells in an in vitro system. Ma-CFCs are grown in EpiCult®-B (Human) Catalog #05601 in conjunction with a layer of irradiated 3T3 fibroblasts (feeders) cells. After a week in culture, Ma-CFCs develop into discrete adherent colonies that can be further analyzed using immunocytochemistry for the presence of epithelial-specific markers (i.e. keratins 14, 18 and 19). Try our NEW human mammary epithelial cell colony interactive tutorial! /VD018_mammary_image.gif)
MammoCult® Medium for Culture of Mammospheres and Tumorspheres
MammoCult® supports the growth of primary human mammary epithelial cells as well as numerous breast cancer cell lines, including MDA-MB-231, SUM149, SK-BR3 and MCF7. It is the only commercially available medium designed specifically to provide you with the highest quality of mammosphere and tumorsphere cultures. Try MammoCult® with your cultures today! /VD025_mammocult_thumbnail.jpg)
MesenCult®-XF for the Culture of Human MSCs
Starting with primary tissue, obtain more MSCs in fewer passages with MesenCult®-XF than with serum-based medium. Learn how.
This video was recorded in October 2009. We have since changed the name of MesenCult®-ACF to MesenCult®-XF.
The product formulation and performance has NOT changed. The change from "animal component-free" to "xeno-free" (XF) nomenclature is to highlight the following feature: xeno-free products are considered "humanized". They may contain ingredients derived from human sources and any recombinant materials must correspond to human genetic sequences.
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MesenCult®-XF Medium for Culture of Mesenchymal Stem Cells
Learn about MesenCult®-XF, a standardized, serum-free and xeno-free medium for the culture of human mesenchymal stem cells.
This video was recorded in October 2009. We have since changed the name of MesenCult®-ACF to MesenCult®-XF.
The product formulation and performance has NOT changed. The change from "animal component-free" to "xeno-free" (XF) nomenclature is to highlight the following feature: xeno-free products are considered "humanized". They may contain ingredients derived from human sources and any recombinant materials must correspond to human genetic sequences. 
MethoCult®: Bone Marrow & Cord Blood – colony ID tutorials
The colony-forming cell (CFC) or colony-forming unit (CFU) assay allows for functional and quantitative assessment of hematopoietic progenitors derived from cord blood, bone marrow and peripheral blood. STEMCELL Technologies’ MethoCult® products are stringently quality controlled and are recognized worldwide as the “Gold Standard” media for CFC (CFU) assays.
MethoCult® Express for Cord Blood Banks and Transplantation Centers
MethoCult® Express permits enumeration of total hematopoietic progenitors (CFC) in cord blood units in only 7 days /MethoCultExpress_thumbnail.jpg)
mTeSR™: A guide to passaging hESC
mTeSR™1 is a defined medium designed for the maintenance of hESC and hiPSC without the use of feeders. This video describes the morphology of cells at different stages during culture and also describes methods to passage cells cultured in mTeSR™1. 
mTeSR®1: Feeder-Independent Culture of hESCs and hiPSCs using mTeSR®1
mTeSR®1 is the most widely published feeder-independent maintenance medium for hESC and hiPSC. It provides a defined and rigorously quality-controlled maintenance medium for hESCs & hiPSCs and completely eliminates the need for feeders.
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Identification and Quantification of Neural Stem and Progenitor Cells with the NeuroCult® NCFC Assay
This video provides an introduction to the NeuroCult® Neural Colony-Forming Cell (NCFC) Assay. The NCFC Assay is a single-step assay for the identification and quantification of neural stem cells and neural progenitor cells based on their proliferative potential.
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NeuroCult® Webinar: Standardized Media and Reagents for Neural Stem Cell Research
This webinar presentation will provide an overview of methods to culture and quantify neural stem and progenitor cells. Specific advantages of culturing neurospheres with NeuroCult® media and quantifying neural stem and progenitor cells with the NeuroCult® Neural Colony-Forming Cell (NCFC) Assay will also be discussed.
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RosetteSep®
Save time. RosetteSep® is a rapid cell separation procedure for isolating highly purified “untouched” cells directly from human whole blood. This video describes how RosetteSep® turns a simple density centrifugation step into a specific antibody-mediated cell enrichment procedure without using columns or magnets. Learn how to isolate T cells, CD4+ T cells, CD8+ T cells, B cells, NK cells, Granulocytes and much more with just a simple spin.
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RosetteSep® for HLA Analysis
Learn how RosetteSep®, a rapid cell separation procedure for isolating highly purified cells directly from whole blood, can help increase your sample processing capabilities and provide clear, reliable HLA test results.
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RosetteSep®: A Guide to Isolating NK Cells Directly from Whole Blood
This video from JoVE features Dr. Christine Beeton from the University of California, Irvine who describes a procedure on how to isolate NK cells directly from human whole blood using the RosetteSep® NK Cell Enrichment Cocktail Kit.
RoboSep® - Product Tour
Simply load and walk away. RoboSep® is the first instrument to offer true walk-away automation of immunomagnetic cell separation. Using column-free EasySep® technology, the RoboSep® performs all steps necessary to magnetically label and separate virtually any cell type by positive or negative selection. This video describes all the features and benefits of RoboSep®, as well as simple instructions on how to select pre-optimized cell separation protocols and load the carousel to start RoboSep®. 
RoboSep® - Sneak Preview
Save time and eliminate sample contamination. The RoboSep® is the first instrument that is designed to minimize sample handling, eliminate cross-contamination, and reduce “hands-on” time - ideal for busy labs studying infectious diseases or requiring high-throughput sample processing. With minimal maintenance and set-up, the RoboSep® is the only fully automated cell separator. 
Standardizing hESC and hiPSC maintenance and differentiation
As therapies based on human pluripotent stem cells (hPSCs) move towards the clinic, there is a need to develop standardized protocols free from animal proteins for maintenance and differentiation. This tutorial will describe the development of TeSR™, animal-protein free medium for the maintenance of hPSCs, the application of AggreWell™ for differentiation to clinically-relevant cell types, and tools for the purification of differentiated cells. Together these reagents provide real solutions to minimize variability and accelerate discovery. /VD026_thumb.gif)
StemSpan® Serum-Free Expansion Media
Learn about StemSpan® media and StemSpan® cytokine cocktails for expansion of hematopoietic stem and progenitor cells. /10001_QV_StemSpan(100211)_thumbnail.jpg)
TeSRTM2 Medium for the culture of human embryonic and induced pluripotent stem cells
Closely related to mTeSR®1, the most-published medium for the culture of hESC and hiPSC without feeders, TeSRTM2 combines the advantages of a feeder-independent culture system with the added value of being free of non-human proteins. /GV001_thumbnail.jpg)
TeSRTM2 Webinar: A Step Closer to Clinically-Compatible Culture of hESCs and hiPSCs:
TeSRTM2, is the latest solution from STEMCELL Technologies for the culture of human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) without the use of feeders. TeSRTM2 combines the advantages of a feeder-independent culture system with the added value of being free of non-human proteins. This video describes the robust validation of TeSR™2 using multiple cells lines over multiple passages and highlights some practical aspects of culturing with TeSRTM2. /video_thumbnail_TeSR.jpg)
Tools for Breast Cancer Resarch
The video provides an overview of high quality tissue culture and cell separation products that STEMCELL Technologies offers to support the studies on breast cancer. Learn about how MammoCult®, ALDEFLUOR® and EpiCult®-B can help your research today.
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