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RosetteSep® is a rapid and easy cell separation procedure for the isolation of highly purified cells directly from whole blood. Utilizing STEMCELL Technologies’ patented antibody-based TAC (tetrameric antibody complex) technology, RosetteSep® turns a simple buoyant density centrifugation (e.g. Ficoll® step) into a specific, antibody-mediated cell enrichment system. Combine Ficoll™ spin and cell isolation into one step Cell enrichment with RosetteSep® simply involves a brief incubation of the sample with the antibody-based enrichment cocktail at room temperature, followed by a standard buoyant density separation. Separate many samples at the same time The RosetteSep® antibody cocktail crosslinks unwanted cells in human whole blood to multiple red blood cells (RBCs), forming immunorosettes (Figure 1). This increases the density of the unwanted (rosetted) cells, such that they pellet along with the free RBCs when centrifuged over a buoyant density medium such as Ficoll-Paque®. Recover more cells from each sample Desired cells are never labeled with antibody and are easily collected as a highly enriched population at the interface between the plasma and the buoyant density medium (Figure 2). Specialized equipment, such as a magnet, is not required and the enriched cells are ready for future use. | 
