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media & supplements
neurospheres
neural colony-forming cell assay kit
support products (including reagents to dissociate neurospheres and primary tissue)
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Mouse embryonic day 14 (E14) neural stem cells (NSCs) are a unique population of cells that exhibit stem cell functions, including self-renewal, production of a large number of progeny and differentiation into neurons, astrocytes and oligodendrocytes.1 Stem cells derived from the mouse E14 CNS can be maintained in an undifferentiated proliferative state by culturing as neurospheres. When removed from serum-free proliferation medium and cultured in medium appropriate for differentiation, the stem cell progeny can be differentiated into the three primary CNS phenotypes: neurons, astrocytes and oligodendrocytes.
NeuroCult® for mouse neural stem and progenitor cells
STEMCELL Technologies has optimized culture conditions that allow mouse CNS stem cells to be maintained for extended periods of time without the loss of their self-renewal, proliferation or differentiation potential.
Cryopreserved mouse passage 1 neurospheres from three different regions of mouse E14 CNS are available. Cryopreserved neurospheres are easy to use, convenient and cost-effective. Researchers can avoid live animal work and laborious preparation time by utilizing prepared neurospheres. Cells within neurospheres or primary cells from the embryonic and adult mouse CNS can be expanded with the NeuroCult® Proliferation Kit (Mouse), which yields significantly increased total cell expansion when compared to a traditional formulation. Mouse neural stem and progenitor cells can be differentiated into neurons, astrocytes and oligodendrocytes by plating cells with the NeuroCult® Differentiation Kit (Mouse).
Figure 1. Culture, Expansion, Differentiation, and Cloning of Neural Stem Cells in NeuroCult® Medium and Supplements
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Figure 2. Differentiation of Neural Stem Cells
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FOR RESEARCH USE ONLY
references
1. Reynolds BA, Weiss A: Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell. Dev Biol 175: 1-13, 1996
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