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cell separation
for human cells
STEMCELL Technologies has a wide range of individual StemSep® antibody tetramers that target a variety of human T cell activation or development markers. These tetramers can be added directly to one of our standard StemSep® T cell cocktails in order to isolate minor T cell subsets. In addition, STEMCELL can incorporate antibodies from other sources into the StemSep® system provided they are either murine IgG1 or biotinylated, allowing researchers the flexibility of designing their own unique cocktails.
Also available is a complete kit for the isolation of CD4+CD25+ T cells, which combines a RosetteSep® CD4+ T cell enrichment with an EasySep® CD25+ selection.



desired T cell subset

 

product(s) required

   
γδ T cells (negative selection) StemSep® T cell enrichment cocktailStemSep® TCR∝β TAC
   
Memory CD4+ T cells (negative selection) StemSep® CD4+ T cell enrichment cocktailStemSep® CD45RA TAC
   
Resting CD4+ T cells (negative selection) StemSep® CD4+ T cell enrichment cocktail • one or more of: StemSep® CD25 TAC, StemSep® HLA-DR TAC
   
Naïve CD4+ T cells (negative selection) StemSep® Naïve CD4+ T cell enrichment cocktail 
   
Naïve CD8+ T cells (negative selection) StemSep® CD8+ T cell enrichment cocktailStemSep® CD45RO TAC
   
Memory CD8+ T cells (negative selection) StemSep® CD8+ T cell enrichment cocktailStemSep® CD45RA TAC
   
Resting CD8+ T cells (negative selection)  StemSep® CD8+ T cell enrichment cocktail • one or more of: StemSep® CD25 TAC, StemSep® HLA-DR TAC
   
CD4+CD25+bright T cells (negative selection of CD4+ T cells, positive selection of CD25+bright cells)


CD25+bright cells



pan-CD25+ cells

 





• RosetteSep® CD4+ T cell enrichment cocktail & EasySep® CD25 positive selection (complete kit)
 


• EasySep® CD25+bright Selection Cocktail • EasySep® Magnetic Nanoparticles


• EasySep® pan-CD25 Selection Cocktail • EasySep® Magnetic Nanoparticles


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StemCell Technologies Inc The Cell Experts