Human CD4+CD25+bright T cells can be isolated quickly and easily directly from whole human blood! CD4+ T cells are first enriched using RosetteSep®, an immunodensity centrifugation procedure. The RosetteSep® reagent is added directly to whole blood, followed by a standard Ficoll® centrifugation step. The CD4+ cells are collected from the Ficoll:plasma interface, while the CD4- cells are pelleted with the red blood cells. CD25+bright cells are then selected from the CD4+ T cell-enriched sample using EasySep®. EasySep® is a simple, column-free magnetic selection procedure that uses FACS-compatible magnetic nanoparticles. In a standard FACS tube, the EasySep® reagents are added to the cells, the tube is placed into the handheld EasySep® magnet, and the unselected (CD4+CD25-/+dim)cells are poured off, leaving the selected (CD4+CD25+bright) cells in the tube and ready for further study. With these two simple methods, highly enriched CD4+CD25+bright T cells are obtained in less than two hours!
In addition, EasySep® CD25+bright positive selection can be fully automated by RoboSep®.
Click here for information on the complete selection kit.
|
