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Does your compound influence regeneration?
Specific compounds inhibit the marrow microenvironment in which the stem cells for various tissue components reside. We can test for inhibition of mesenchymal proliferation using the quantitative CFU-F assay.
Effect of chemotherapeutics on human CFU-F growth
| | Drug Concentration | % of Control Growth | 
Control
| Hydroxyurea | 1 µM
5 µM
25 µM
50 µM
100 µM | 111 ± 9
111 ± 14
96 ± 14
85 ± 14
50 ± 27 | 
50 µM | Paclitaxel | 0.1 ng/mL
0.5 ng/mL
1 ng/mL
5 ng/mL
10 ng/mL | 112 ± 23
92 ± 37
76 ± 30
3 ± 4
1 ± 1 | 
1 ng/mL | 5-Fluorouracil | 0.05 µg/mL
0.1 µg/mL
0.5 µg/mL
1 µg/mL
5 µg/mL | 87 ± 15
78 ± 24
3 ± 4
2 ± 2
0 | 
0.1 µg/mL |
Does your compound cause myelosuppression?
Chemotherapeutics are known to cause myelosuppression, but small molecule compounds targeted for the treatment of non-hematological malignancies may also suppress either myeloid or erthroid progenitor proliferation.
Effect of chemotherapeutics on erythroid (CFU-E and BFU-E) and myeloid (CFU-GM) growth
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