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Does your compound support the maintenance of primitive progenitors?
Cell expansion is hampered by the terminal differentiation of cells in the cell population. We can use the LTC-IC assay to determine if small molecule compounds assist in maintaining the primitive cell pool.
Does your compound have potential cytokine activity?
Small molecule compounds can be tested for their ability to directly induce progenitor proliferation or their ability to synergize with known cytokines and enhance their effect.
Effect of EPO concentration on erythroid colony number in various cytokine formulations in MethoCult®
Concentration EPO Added | CFU-E | BFU-E | Total Erythroid Colonies | | MethoCult® H4230 (no cytokines) | | | 20 mU/mL | 2 ± 1 | 0 ± 0 | 2 ± 1 | | 200 mU/mL | 8 ± 3 | 16 ± 3 | 24 ± 1 | | 2000 mU/mL | 3 ± 2 | 23 ± 1 | 27 ± 2 | | MethoCult® H4230 + 100 ng/mL SCF | | | 20 mU/mL | 3 ± 2 | 15 ± 7 | 19 ± 8 | 200 mU/mL | 1 ± 1 | 32 ± 3 | 33 ± 3 | 2000 mU/mL | 1 ± 1 | 35 ± 8 | 36 ± 8 | | MethoCult® H4230 + 20 ng/mL IL-3 | | 20 mU/mL | 8 ± 2 | 8 ± 1 | 16 ± 2 | 200 mU/mL | 9 ± 5 | 24 ± 5 | 33 ± 7 | 2000 mU/mL | 5 ± 3 | 30 ± 7 | 35 ± 9 | | MethoCult® H4230 + 20 ng/mL IL-6 | | | 20 mU/mL | 2 ± 2 | 1 ± 1 | 2 ± 2 | 200 mU/mL | 8 ± 2 | 16 ± 3 | 24 ± 4 | 2000 mU/mL | 3 ± 1 | 24 ± 3 | 27 ± 4 |
Effect of EPO on erythroid colony growth with saturating concentrations of SCF, IL-3, and GM-CSF
0.03 U/mL EPO | 
0.3 U/mL EPO | 
3 U/mL EPO |
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