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The role of the HLA (Human Leukocyte Antigen) complex is of critical importance in immune responses and transplant medicine. Since the late 1960s, research on human organ and tissue transplantation reinforced the importance of HLA matching for graft survival and transfusion efficiency. Today, HLA testing is a standard procedure that is important in organ and hematopoietic transplantation. The specific and varied distribution of HLA antigens requires precise, accurate, and consistent HLA testing. In a busy HLA lab, finding powerful tools that facilitate accurate HLA testing while processing a high number of samples is important. At STEMCELL TechnologiesTM, we provide highly optimized cell separation solutions for a variety of HLA applications to facilitate high-volume sample processing and clear, reliable test results. Our HLA cell isolation products have been formulated for: • Serology • Flow Cytometry Crossmatch (FCXM) • Chimerism Analysis
Our cell isolation systems are particularly suitable for HLA labs as all procedures are: • Gentle on cells • Quick • Easy • Performed at room temperature
Click here to see a comparison of 3 cell isolation methods for serological HLA Class I typing
Advantages of RosetteSep® include:
*High purity and high recovery of specific cell types
*Procedure involves only a brief room temperature incubation of the blood sample with RosetteSep® reagent prior to a FicollTM spin
* No magnets or other equipment required
* Minimal sample handling
* For use with samples containing red blood cells
* We offer a Granulocyte Depletion Reagent for use with older blood samples where granulocyte contamination may be a problem.
Products For:
Total Lymphocyte Enrichment • T Cell Enrichment • B Cell Enrichment • Granulocyte Depletion • Lymphoid Enrichment Kit for Lineage-Specific Chimerism Analysis• Myeloid Enrichment Kit for Lineage-Specific Chimerism Analysis
Advantages of EasySep® include:
* High purity of specific cell types
* Isolated cells are immediately available for flow cytometry cross matching, serology analysis, and any other downstream assay
* For use with mononuclear cell suspensions or cryopreserved samples, or for positive selection directly from whole blood
* No columns! Requires only a unique handheld EasySep® magnet and economical cell selection reagents RoboSep® offers all the advantages of EasySep® in a fully automated system, Plus:
* Labels and separates up to 4 independent samples at one time
* Each sample is handled with a unique set of disposable tips to eliminate cross contamination
* Every stage of the process is fully automated to minimize sample handling
EasySep® and RoboSep® products:
For any sample:
CD2+ Cell Selection • CD3+ Cell Selection • CD19+ Cell Selection • T Cell Enrichment • B Cell Enrichment
For positive selection directly from whole blood:
CD2+ Cell Selection • CD3+ Cell Selection • CD4+ T Cell Selection • CD8+ T Cell Selection • CD15+ Cell Selection • CD19+ Cell Selection • CD33+ Cell Selection • CD66b+ Cell Selection • Myeloid Cell Selection • Lymphoid Selection
For positive selection from buffy coat:
CD56+ Cell Selection
For enrichment from whole blood (using HetaSepTM sedimentation):
Total lymphocyte enrichment • T Cell enrichment • B Cell enrichment
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