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Video - EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type

What is EasySep^®?
EasySep^® is a powerful immunomagnetic cell selection procedure that uses specific antibodies and tiny FACS-compatible magnetic nanoparticles in a column-free magnetic system.  Cells are targeted for selection or depletion using monoclonal antibodies directed against specific cell surface antigens.  These labeled cells are then crosslinked to EasySep^® magnetic nanoparticles in a standard FACS tube.  The tube is then placed directly in the specially designed EasySep^® magnet. This handheld magnet is gently inverted to remove the cells that are not bound to the magnetic nanoparticles.  The unique EasySep^® magnet generates a high gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with EasySep^® nanoparticles without the additional magnetic field gradients provided by a column matrix. Unlike larger microparticles used with other column-free systems, the EasySep^® nanoparticles do not interfere with subsequent FACS analysis and do not need to be removed.

For Positive Cell Selection:  Cells of interest remain in the tube after pouring off the cells that are not bound to the magnetic nanoparticles. To obtain a highly pure population, these cells are rinsed twice or more, then easily collected by removing the tube from the magnet.  For products for positive selection, see below.

For Negative Enrichment:  Cells of interest are poured into a new tube when the magnet is inverted, as they are not bound to the magnetic nanoparticles. For products for negative enrichment, see below.

Easily Automated with RoboSep: EasySep^® procedures can  be performed manually by one of our EasySep^® magnets, or by using the RoboSep^® the automated cell separator.

Figure 1.  Schematic drawing of EasySep^®  magnetic labeling of human cells



Figure 2. General EasySep^® procedure

Easy column-free magnetic positive or negative cell selection

PRODUCTS FOR:

Positive selection of HUMAN cells
CD3 • CD4 • CD8 • CD14 • CD19 • CD33 • CD34 • CD45 • CD56 • CD138 • EpCAM • CD10 • MUC1 • GlyA • PE selection • FITC selection • APC Selection • Biotin selection • Or your own IgG₁ antibody

Positive selection of HUMAN cells directly from WHOLE BLOOD
CD3 • CD4 • CD8 • CD14 • CD15 • CD19 • CD33 • CD34 • CD56 • CD66b • CD138 • Myeloid • Lymphoid • CD45 Depletion

Negative selection of HUMAN cells
T cells • CD4⁺ T cells • Naïve CD4⁺ T cells •  Memory CD4⁺ T cells • CD8⁺ T cells • B cells • NK cells • Monocytes • Monocytes without CD16 Depletion • Progenitor cells • Progenitor cells with CD41 depletion • Eosinophils • Basophils • Neutrophils • Dendritic cells • Plasmacytoid DC • CD4⁺CD127^low T Cell

Positive selection of MOUSE cells
CD90.2 • CD4 • CD8 • CD11b • CD11c • CD19 • Regulatory (CD4⁺CD25⁺) T cell • NK • SCA1 • SCA1 Biotin • CD117 (cKIT) • PE Selection • FITC Selection • APC Selection • Biotin Selection

Negative selection of MOUSE cells
T cells • CD4⁺ T cells • CD8⁺ T cells • B cells • NK Cells • Hematopoietic Progenitor Cells • Mammary Stem Cells • Mesenchymal Progenitor Cells • Monocytes

Positive selection of ANY cells
PE selection • FITC selection • APC Selection • Biotin selection • Or your own IgG₁ antibody

PLUS! Click here to ask us about customized EasySep^® cell separation products!