Related Links Recent Publications ClonaCell™-HY Manual Mini-Review

Monoclonal antibody production made easy! Since the first derivation of monoclonal antibodies of defined specificity in 1975, a variety of methods have been used to grow, select, and clone antibody-producing hybridomas. Many of the approaches are time-consuming and labor-intensive. Following fusion and growth in selective liquid medium, limiting dilutions are required to obtain monoclonal hybridomas. A problem often encountered is that some hybridomas overgrow others prior to cloning. Often, these faster growing cells do not synthesize antibodies, resulting in a failure to obtain the best antibody-producing hybridomas. A solution to these problems is to clone the hybridomas immediately after fusion using a selective semi-solid medium.

ClonaCell^®-HY simultaneous selection and cloning system centers around a methylcellulose-based semisolid medium containing selective agents, growth factors and supplements optimized to support the selection and growth of hybridoma clones. Using ClonaCell™-HY, selection and cloning of hybridoma cells begins within 24 hours of fusion.

products
ClonaCell^®-HY Complete Kit
ClonaCell^®-HY Pre-Fusion Medium A
ClonaCell^®-HY Fusion Medium B
ClonaCell^®-HY Recovery Medium C
ClonaCell^®-HY Selection and Cloning Medium D
ClonaCell^®-HY Growth Medium E
ClonaCell^®-HY PEG
ClonaCell^®-HY Liquid HAT Selection Medium

ClonaCell^®-HY Procedure




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