We can evaluate any stimulatory or inhibitory effects of your compound in hematopoietic colony assays using bone marrow progenitor cells from intact bones from your mouse experiments.

Drug toxicity studies in rat or mouse bone marrow
> In vivo or in vitro administration of compounds
> Complete blood cell counts
> Serum ELISAs
> Marrow toxicity

In vivo treatment of mice with chemotherapeutic agent induces decreased bone marrow cellularity and affects progenitor proliferative potential:

	Control Bone Marrow		5-FU Treated Bone Marrow
Day	Cells/Femur (x 106)	CFC/Femur	Cells/Femur (x 106)	CFC/Femur
2	18.2	107 557	6.4	2572
3	17.6	86 191	5.2	8272
7	18.8	88 407	5.3	59 584
8	12.8	88 527	7.7	69 030
9	17.6	72 160	17.4	80 040
11	17.3	58 752	17.5	45 500

In vitro treatment of mouse bone marrow with chemotherapeutic agent affects progenitor proliferative potential:

CFU-GM in control culture

CFU-GM in culture incubated with 5-FU

In vivo hematopoietic modulating activity
> Stem cell mobilization
> Tri-lineage engraftment
> Complete blood cell counts
> Bone marrow or peripheral blood CFC and progenitor numbers

Phenotype characterization
> Gross examination
> Progenitor analysis
> Stress/recovery analysis
> immunophenotypic analysis