STEMCELL :: Home :: News and Information 2009

technical info & literature

ordering information

communication zone


	My Cart- My Account- Login

Home -- News and Information 2009 -- TeSR™2

News and Information 2009

TeSR™2

	TeSR™2
	Animal Protein-Free Maintenance Medium for hESCs & hiPSCs

	•	Animal Protein-Free, defined
	•	Reduced variability in hESC and hiPSC culture
	•	No need to grow feeders

Now Available!

TeSR™2, is the latest solution from STEMCELL Technologies for the culture of human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) without the use of feeders (Figure 1). Closely related to mTeSR^®1, the most-published medium for the culture of hESC and hiPSC without feeders, TeSR™2 combines the advantages of a feeder-independent culture system with the added value of being free of non-human proteins.


Figure 1: hiPSCs cultured in TeSR™2 grow as colonies with defined edges and high nucleus to cytoplasm ratio. iPS(IMR90)-1 cell line cultured (A) for 8 passages in TeSR™2 and (B) for 10 passages in TeSR™2. (Photo curtesy of Dr. T. Ludwig, WiCell Research Institute)	Figure 2: Consistent expansion of hESCs cultured in TeSR™2. H1 and H9 culls were cultured in TeSR™2 for 26 and 27 passages respectively. Average fold expansion (±SEM) over these passages are shown.

Based on a published formulation¹, TeSR™2 includes high levels of bFGF² together with transforming growth factor ß (TGFß - to inhibit BMP signaling)³, gamma-aminobutyric acid (GABA), pipecolic acid and lithium chloride. The medium allows for the long-term maintenance of undifferentiated hESCs and hiPSCs (Figure 2) with full retention of pluripotency (Figures 3 and 5) and normal karyotype (Figure 4).

The development of fully humanized and defined systems for the maintenance of hESCs and hiPSCs is very important with the increasing in developing cellular therapies based on these cells. TeSR™2 represents a significant step in this direction.

Figure 3: Flow Cytometric Analysis of H1 cells maintained in TeSR™2 for 26 passages. Figure 4: Retention of normal karyotype in (A) H1 and (B) H9 cells following long term passaging in TeSR™2, 19 and 22 passages respectively.

Figure 5: H9 cells were cultured for 11 passages in TeSR™2 then injected subcutaneously into NOD-SCID mice. The resulting teratomas contained cell types from all 3 germ layers.⁴ Representative tissue types are shown.

ORDERING INFORMATION

PRODUCT	QUANTITY	CATALOG #
TeSR™2	500 mL	05860
TeSR™2	10 x 500 mL	05880

References:

1.	Ludwig TE et al, Nat Biotechnol 24: 185-187, 2006
2.	Xu RH et al, Nature Methods. 3:185-190, 2005
3.	Levenstein ME et al, Stem Cells 3:568-574, 2006
4.	Thomson JA et al, Science 5391:1145-1147, 1998

	Email this page \| Print this page
	Copyright © 2006 StemCell Technologies Powered by: GreenButton inc.

StemCell Technologies Inc The Cell Experts