Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood

Publish Date: September 08, 2009     Duration: 1:30     Views: 1890

Description

This video provides step-by-step instructions on how to isolate mononuclear cells from whole blood using a density gradient medium such as Ficoll-Paque™ PLUS. Watch the procedure being performed, which includes techniques such as layering diluted blood over Ficoll-Paque™ PLUS and collecting desired cells from the plasma/Ficoll-Paque™ PLUS interface. Ficoll-Paque™ PLUS can be used either prior to downstream cell separations with EasySep® or in conjunction with RosetteSep®. Watch other videos here.

Products in this Video

• EasySep HLA B Cell Enrichment Complete Processing Kit for Whole Blood
• EasySep Human PanDC PreEnrichment Kit
• EasySep Human Nave CD4 T Cell Enrichment Kit
• EasySep HLA T Cell Enrichment Kit
• EasySep Human CD8 T Cell Enrichment Kit
• EasySep Human CD14 Positive Selection Kit
• EasySep Mouse CD117 cKIT Positive Selection Kit
• EasySep Human PanCD25 Positive Selection and Depletion Kit
• EasySep Human CD25 Positive Selection Kit
• EasySep Mouse CD11b Positive Selection Kit
• EasySep Biotin Positive Selection Kit
• EasySep Multistand
• The Big Easy EasySep Magnet
• EasySep Mouse T Cell Enrichment Kit
• EasySep Human Whole Blood CD8 Positive Selection Kit
• EasySep Mouse CD8 T Cell Enrichment Kit
• EasySep Human CD3 Positive Selection Kit
• EasySep Mouse Epithelial Cell Enrichment Kit
• EasySep Human Neutrophil Enrichment Kit
• EasySep Human Whole Blood Lymphoid Positive Selection Kit
• EasySep Mouse CD902Thy 12 Positive Selection Kit
• EasySep Magnet
• EasySep HLA Whole Blood CD2 Positive Selection Kit
• EasySep Mouse Hematopoietic Progenitor Cell Enrichment Kit
• EasySep HLA Whole Blood Myeloid Positive Selection Kit
• FicollPaque PLUS
• EasySep Human T Cell Enrichment Kit
• EasySep Human Cord Blood CD34 Positive Selection Kit
• EasySep Mouse CD4 T Cell Enrichment Kit
• EasyPlate EasySep Magnet
• EasySep HLA Whole Blood T Cell Enrichment Kit
• EasySep HLA B Cell Enrichment Kit
• EasySep Mouse Mesenchymal StemProgenitor Cell Enrichment Kit
• EasySep Mouse Monocyte Enrichment Kit
• EasySep Mouse CD19 Positive Selection Kit
• EasySep Human Nave CD8 T Cell Enrichment Kit
• EasySep Human CD8 Positive Selection Kit
• EasySep HLA Total Lymphocyte Enrichment Complete Processing Kit for Whole Blood
• EasySep Human Buffy Coat CD14 Positive Selection Kit
• EasySep hESCDerived CD34 Positive Selection Kit
• EasySep Human CD4 T Cell Enrichment Kit
• EasySep HLA CD3 Positive Selection Kit
• EasySep Mouse APC Positive Selection Kit
• EasySep Human Whole Blood CD15 Positive Selection Kit
• Easy 50 EasySep Magnet
• EasySep Mouse PE Positive Selection Kit
• EasySep Human B Cell Enrichment Kit
• EasySep HLA Whole Blood Lymphoid Positive Selection Kit
• EasySep Human Whole Blood CD19 Positive Selection Kit
• EasySep Mouse panNK CD49b Positive Selection Kit
• EasySep Human CD56 Positive Selection Kit
• EasySep Human CD19 Positive Selection Kit
• EasySep Human Myeloid Positive Selection Kit
• EasySep Human Basophil Enrichment Kit
• EasySep Mouse SCA1 Positive Selection Kit
• EasySep DoItYourself Selection Kit
• EasySep Human DoItYourself Selection Kit
• EasySep Mouse Mammary Stem Cell Enrichment Kit
• EasySep Human EpCAM Positive Selection Kit
• EasySep Mouse Custom Positive Selection Kit
• EasySep Human B Cell Enrichment Kit without CD43 Depletion
• EasySep Mouse SCA1 Biotin Positive Selection Kit
• EasySep Human Positive Glycophorin A Depletion Cocktail
• EasySep Human Memory B Cell Isolation Kit
• EasySep HLA Buffy Coat CD56 Positive Selection Kit
• EasySep Human PE Positive Selection Kit
• EasySep Human Plasmacytoid DC Enrichment Kit
• EasySep Human Whole Blood CD33 Positive Selection Kit
• EasySep HLA Whole Blood CD15 Positive Selection Kit
• EasySep HLA Whole Blood CD3 Positive Selection Kit
• EasySep Human Whole Blood CD3 Positive Selection Kit
• EasySep Human CD45 Depletion Kit
• EasySep Human CD33 Positive Selection Kit
• EasySep Human Memory CD4 T Cell Enrichment Kit
• EasySep Human FITC Positive Selection Kit
• EasySep Mouse CD93 AA41 Positive Selection Kit
• EasySep Mouse FITC Positive Selection Kit
• EasySep Human CD4CD127low T Cell Enrichment Kit
• EasySep FITC Positive Selection Kit
• EasySep Human NK Cell Enrichment Kit
• EasySep Human CD2 Positive Selection Kit
• EasySep Mouse CD11c Positive Selection Kit
• EasySep Human Biotin Positive Selection Kit
• EasySep Mouse CD4CD25 Regulatory T Cell Isolation Kit
• EasySep Human CD271 Selection Kit
• EasySep Human Progenitor Cell Enrichment Kit
• EasySep APC Positive Selection Kit
• EasySep Human CD34 Positive Selection Kit
• EasySep Human APC Positive Selection Kit
• EasySep Human Monocyte Enrichment Kit without CD16 Depletion
• EasySep Human CD10 Positive Selection Kit
• EasySep Human Buffy Coat CD56 Positive Selection Kit
• EasySep Human Whole Blood CD34 Positive Selection Kit
• EasySep Human CD4 Positive Selection Kit
• EasySep Human CD138 Positive Selection Kit
• EasySep HLA Whole Blood CD33 Positive Selection Kit
• EasySep Human Whole Blood CD4 Positive Selection Kit
• EasySep Mouse CD8a Positive Selection Kit
• EasySep Mouse Biotin Positive Selection Kit
• EasySep Human Whole Blood CD20 Positive Selection Kit
• EasySep Mouse Custom Enrichment Kit
• EasySep Human Whole Blood Myeloid Positive Selection Kit
• EasySep Human Whole Blood CD138 Positive Selection Kit
• EasySep Mouse B Cell Enrichment Kit
• EasySep HLA CD2 Positive Selection Kit
• EasySep PE Positive Selection Kit
• EasySep Mouse CD4 Positive Selection Kit
• EasySep Mouse NK Cell Enrichment Kit
• EasySep Human Whole Blood CD2 Positive Selection Kit
• EasySep Human MUC1 Positive Selection Kit
• EasySep Human Whole Blood CD66b Positive Selection Kit
• EasySep Human Monocyte Enrichment Kit
• EasySep Human Eosinophil Enrichment Kit
• EasySep Human Naive B Cell Enrichment Kit
• EasySep Human Whole Blood CD45 Depletion Kit