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September 25, 2009
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A Fast and Simple Hematopoietic Colony-Forming Cell Assay for Cord Blood Banks
This video discusses the colony-forming cell (CFC) or colony-forming unit (CFU) assays, the most commonly used assays to measure the frequency and proliferative ability of hematopoietic cells in cell preparations used for transplantation. In unrelated umbilical cord blood (CB) transplantation, total CFC numbers after thawing of CB units has been demonstrated to correlate more strongly with recovery and survival than other parameters, such as CFC numbers before cryopreservation, TNC counts and CD34+ cell numbers. To measure the total CFC content of a cord blood sample, it is not necessary to distinguish colony types, and therefore it is not necessary to culture the cells for 14 -16 days until colonies have fully matured. In this webinar, we will discuss MethoCult® Express. This new MethoCult® medium is formulated for faster CFC assays for hematopoietic progenitors in CB, which allows total colonies to be counted as early as after 7 days, instead of the 14 - 16 days of conventional CFC assays. Watch other videos here.
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Duration: 38:23 min
Views: 344
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September 16, 2010
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A Guide to Erythropoiesis - EPO Signaling and Erythroid Development
This webinar video provides an overview of erythroid development and erythropoietin (EPO) signaling pathways. Methods to measure EPO activity and screening for EPO mimetics will also be covered. Watch other videos here.
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Duration: 40:56 min
Views: 114
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March 22, 2011
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A Guide to Passaging Human Pluripotent Stem Cells Using mTeSR™1
mTeSR™1 is a defined medium designed for the maintenance of hESCs and hiPSCs without the use of feeders. This video describes the morphology of cells at different stages during culture and also describes the method to passage cells cultured in mTeSR™1. Watch other videos here.
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Duration: 5:57 min
Views: 1758
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October 20, 2009
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A Step Closer to Clinically-Compatible Culture of hESCs and hiPSCs with TeSR™2
TeSR™2 , is the latest solution from STEMCELL Technologies for the culture of human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) without the use of feeders. TeSRTM2 combines the advantages of a feeder-independent culture system with the added value of being free of non-human proteins. This video describes the robust validation of TeSR™2 using multiple cells lines over multiple passages and highlights some practical aspects of culturing with TeSRTM2. Watch other videos here.
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Duration: 33:01 min
Views: 97
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August 19, 2011
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Affordable, Defined hPSC Culture? StemAdhere™ “Sticks it” to Other Matrices
StemAdhere™ Defined Matrix for hPSC, developed and manufactured by Primorigen Biosciences®, is a defined, humanized matrix that supports the long-term culture of human pluripotent stem cells (hPSCs). It is an affordable alternative to the commonly used Matrigel™ and may be used in combination with mTeSR™1 or TeSR™2 media for a completely defined, feeder-independent system. Cells easily and conveniently transition from Matrigel™ to StemAdhere™ Defined Matrix for hPSC and are maintained and passaged, thereafter, using a convenient and simple, enzyme-free protocol. Watch other videos here.
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Duration: 36:46 min
Views: 62
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June 19, 2009
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AggreWell™ - A New Technology for Uniform Embryoid Bodies
This webinar video by Dr. Mark Ungrin describes how AggreWell™ can be used to make uniform embryoid bodies from hESC and hiPSC as well as other cell types. Watch other videos here.
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Duration: 24:10 min
Views: 293
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June 19, 2009
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AggreWell™: Are You Making Embryoid Bodies?
This video introduces a new and innovative way of make uniform, size-controlled embryoid bodies. Watch other videos here.
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Duration: 1:00 min
Views: 1583
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July 20, 2010
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ALDEFLUOR® and Cancer Stem Cells
This webinar video explains the principles of ALDEFLUOR® technology, offers literature review and summary of technical considerations of performing and interpreting ALDEFLUOR® assays on primary tissues and tumor cell lines. Watch other videos here.
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Duration: 47:13 min
Views: 605
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September 09, 2009
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ALDEFLUOR®: Detect Normal and Cancer Precursor Cells
An innovative, antibody-free system to rapidly identify stem and progenitor cells of several tissue types, including a variety of cancer stem cells. Watch this video to learn how the ALDEFLUOR® system works. Watch other videos here.
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Duration: 1:25 min
Views: 2386
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August 10, 2011
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Animal Component-Free hPSC Differentiation to Multiple Lineages
This webinar introduces STEMdiff™ APEL™ Medium, a defined, serum-free and animal component-free medium specifically developed to support human pluripotent stem cell (hPSC) differentiation. It was first described for the induction of hemato-endothelial cells, but it has also been proven to be an effective basal medium for hPSC differentiation to other lineages. Learn more about how this medium has been used for hPSC differentiation to cardiomyocyte, hematopoietic, and definitive endoderm, as well as how STEMdiff™ APEL™ Medium, with the right combination of growth factors, may be used for differentiation to other cell lineages either as embryoid bodies (EBs) or in adherent cell-based systems. Watch other videos here.
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Duration: 37:02 min
Views: 26
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