Videos & Webinars

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116 results available

Publish DateMultimedia Title
September 25, 2009
A Fast and Simple Hematopoietic Colony-Forming Unit Assay for Cord Blood Banks

This video discusses the colony-forming cell (CFC) or colony-forming unit (CFU) assays, the most commonly used assays to measure the frequency and proliferative ability of hematopoietic cells in cell preparations used for transplantation. In unrelated umbilical cord blood (CB) transplantation, total CFC numbers after thawing of CB units has been demonstrated to correlate more strongly with recovery and survival than other parameters, such as CFC numbers before cryopreservation, TNC counts and CD34+ cell numbers. To measure the total CFC content of a cord blood sample, it is not necessary to distinguish colony types, and therefore it is not necessary to culture the cells for 14 -16 days until colonies have fully matured. In this webinar, we will discuss MethoCult™ Express. This new MethoCult™ medium is formulated for faster CFC assays for hematopoietic progenitors in CB, which allows total colonies to be counted as early as after 7 days, instead of the 14 - 16 days of conventional CFC assays. Watch other videos here.

September 16, 2010
A Guide to Erythropoiesis - EPO Signaling and Erythroid Development

This webinar video provides an overview of erythroid development and erythropoietin (EPO) signaling pathways. Methods to measure EPO activity and screening for EPO mimetics will also be covered. Watch other videos here.

March 22, 2011
A Guide to Passaging Human Pluripotent Stem Cells Using mTeSR™1

mTeSR™1 is a defined medium designed for the maintenance of hESCs and hiPSCs without the use of feeders. This video describes the morphology of cells at different stages during culture and also describes the method to passage cells cultured in mTeSR™1. Watch other videos here.

November 30, 2012
A Smarter Way To Clone: What You Don't Know About Limiting Dilution Can Hurt Your Probability of Monoclonality

In this webinar, Dr. Jenna Capyk provides a comparison of limiting dilution cloning and semi-solid cloning with respect to their application to cell line development and the probability of monoclonality for the cultures that are isolated using these methods. Watch other videos here.

October 20, 2009
A Step Closer to Clinically-Compatible Culture of hESCs and hiPSCs with TeSR™2

TeSR™2 , is the latest solution from STEMCELL Technologies for the culture of human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) without the use of feeders. TeSR™2 combines the advantages of a feeder-independent culture system with the added value of being free of non-human proteins. This video describes the robust validation of TeSR™2 using multiple cells lines over multiple passages and highlights some practical aspects of culturing with TeSR™2. Watch other videos here.

October 24, 2012
A Technical Guide to the Successful Mucociliary Differentiation of Primary Bronchial Epithelial Cells with PneumaCult™-ALI

This video demonstrates how to differentiate primary human bronchial epithelial cells into a pseudostratified mucociliary epithelium using air-liquid interface culture techniques and PneumaCult™-ALI medium. Watch other videos here.

August 19, 2011
Affordable, Defined hPSC Culture? StemAdhere™ “Sticks it” to Other Matrices

StemAdhere™ Defined Matrix for hPSC, developed and manufactured by Primorigen Biosciences®, is a defined, humanized matrix that supports the long-term culture of human pluripotent stem cells (hPSCs). It is an affordable alternative to the commonly used Matrigel™ and may be used in combination with mTeSR™1 or TeSR™2 media for a completely defined, feeder-independent system. Cells easily and conveniently transition from Matrigel™ to StemAdhere™ Defined Matrix for hPSC and are maintained and passaged, thereafter, using a convenient and simple, enzyme-free protocol. Watch other videos here.

June 19, 2009
AggreWell™ - A New Technology for Uniform Embryoid Bodies

This webinar video by Dr. Mark Ungrin describes how AggreWell™ can be used to make uniform embryoid bodies from hESC and hiPSC as well as other cell types. Watch other videos here.

June 19, 2009
AggreWell™: Are You Making Embryoid Bodies?

This video introduces a new and innovative way of make uniform, size-controlled embryoid bodies. Watch other videos here.

July 20, 2010
ALDEFLUOR™ and Cancer Stem Cells

This webinar video explains the principles of ALDEFLUOR™ technology, offers literature review and summary of technical considerations of performing and interpreting ALDEFLUOR™ assays on primary tissues and tumor cell lines. Watch other videos here.