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iPS Cell Training Course: Reprogramming Somatic Cells to iPS Cells

Reprogramming Somatic Cells to iPS Cells Training Course

  • iPS Cell Training Course
iPS Cell Training Course
Product Catalog # Size Price
iPS Cell Training Course
Two-day training course on reprogramming of somatic cells to iPS cells.
00224 1 course Request pricing

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This two-day course is focused on deriving and maintaining human induced pluripotent stem cells (iPS cells) from somatic cells using a defined and feeder-free system. An overview of popular reprogramming methods will be provided as pre-course material to prepare students for classroom discussions. Preparation, purification, expansion and reprogramming of somatic cells such as blood cells or fibroblasts will be demonstrated with an emphasis on identifying and isolating iPS colonies. Over half of the course will be devoted to hands-on laboratory techniques lead by expert scientists and educators from STEMCELL Technologies, with the main focus on identifying and maintaining fully reprogrammed iPS cell colonies.
Personalized instruction enables students to master the entire process of reprogramming from somatic cell preparation and transfection, to iPS cell identification and isolation. Students will also learn techniques for maintenance of iPS cells and forming uniformly-sized embryoid bodies for downstream applications.
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Product Type: Training & education
Recommended For:
• Scientists interested in working with a standardized, serum- and feeder-free system to reprogram somatic cells to iPS cells
• Scientists interested in maintaining iPS cells in serum- and feeder-free culture media such as mTeSR™1 and TeSR™-E8™
• Scientists interested in gaining familiarity with isolating CD34+  cells from peripheral blood
• Isolation, selection, and expansion of blood and fibroblast cells prior to reprogramming
• Reprogramming somatic cells into iPS cells
• Feeder-free maintenance of iPS cell cultures using mTeSR™1 or TeSR™-E8™ media, and Matrigel™ or Vitronectin XF™ matrices
• Characterization of iPS cell colonies
• Methods to generate embryoid bodies for differentiation, including AggreWell™
• Isolate peripheral blood mononuclear cells with SepMate™ and enrich CD34+ cells using EasySep™
• Transfection of somatic cells with reprogramming factors
• Observe morphological changes during the reprogramming process using TeSR™-E7™
• Identify and mechanically select fully reprogrammed iPS cell colonies
• Passage ES and iPS cell cultures in mTeSR™1 & Matrigel™ or Vitronectin XF™ & TeSR™-E8™ using Gentle Cell Dissociation Reagent
• Remove differentiated areas during passaging
• Cryopreserve and thaw iPS cells
• Create uniformly-sized embryoid bodies using AggreWell™
• Dinner with STEMCELL Technologies' experts
• Lunches and refreshments served daily
• Course booklet and electronic versions of instructional material
• Certificate issued upon successful completion of the training course
•Post laboratory Q&A and quiz on second day
STEMCELL Technologies
1618 Station Street
Vancouver, BC, Canada
V6A 1B6
Area of Interest: Embryonic stem cell & iPS cell research
Cell Type: Embryonic stem cells & iPS cells
Training & Education Type: Training courses

Technical Resources

Protocols and Manuals

Current documents
Product NameLot # ?Document TypeDownload
iPS Cell Training Course: Reprogramming Somatic Cells to iPS Cells All Manual
iPS Cell Training Course: Reprogramming Somatic Cells to iPS Cells All Manual

Educational Resources

Videos & Webinars

  • Choosing the Right Medium for Your hPSC Culture
  • Brochures

  • Products for Human Pluripotent Stem Cells
  • TeSR™-E7™ Reprogramming Medium for Human iPS Cell Induction
  • Scientific Posters

  • Reprogramming of somatic cells to induced pluripotent stem cells in TeSR™-E7™, a feeder-free, defined, and low-protein medium



    Related Products

    Other Reagents and Materials

    Product Name


    Catalog #

    mTeSR™1 Defined, Feeder-Free Maintenance Medium for Human ES and iPS Cells 05850
    TeSR™-E7™ Feeder-Free, Xeno-Free Reprogramming Medium for Human iPS Cell Induction 05910
    Vitronectin XF™ Defined, Xeno-Free Cell Attachment Factor that Supports the Growth and Differentiation of Human Pluripotent Stem Cells Under Serum-Free, Feeder-Free Conditions 07180
    AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
    CryoStor® CS10 Animal Component-Free, Defined Cryopreservation Medium 07930
    STEMdiff™ Neural Rosette Selection Reagent Enzyme-Free Reagent for the Selective Detachment of Neural Rosette Structures 05832
    STEMdiff™ Neural Progenitor Medium For Maintenance and Expansion of Neural Progenitor Cells Derived from Human ES and iPS Cells 05833
    STEMdiff™ Neural Progenitor Freezing Medium For Cryopreservation of Neural Progenitor Cells Generated Using STEMdiff™ Neural Induction Medium 05838
    STEMdiff™ Definitive Endoderm Kit Fully Defined Animal Component-Free Medium for the Differentiation of Human ES and iPS Cells to Definitive Endoderm. 05110

    Training and Education

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    Catalog #

    Differentiation of Human ES and iPS Cells to Neural Progenitor Cells Training Course Differentiation of Human ES Cells and iPS Cells to Neural Progenitor Cells Training Course 00219 Request pricing


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