iPS Cell Training Course: Reprogramming Somatic Cells to iPS Cells

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Reprogramming Somatic Cells to iPS Cells Training Course

  • iPS Cell Training Course
iPS Cell Training Course
This two-day course is focused on deriving and maintaining human induced pluripotent stem cells (iPS cells) from somatic cells using a defined and feeder-free system. An overview of popular reprogramming methods will be provided as pre-course material to prepare students for classroom discussions. Preparation, purification, expansion and reprogramming of somatic cells such as blood cells or fibroblasts will be demonstrated with an emphasis on identifying and isolating iPS colonies. Over half of the course will be devoted to hands-on laboratory techniques lead by expert scientists and educators from STEMCELL Technologies, with the main focus on identifying and maintaining fully reprogrammed iPS cell colonies.
Personalized instruction enables students to master the entire process of reprogramming from somatic cell preparation and transfection, to iPS cell identification and isolation. Students will also learn techniques for maintenance of iPS cells and forming uniformly-sized embryoid bodies for downstream applications.
 
Note: This course is hosted in Vancouver following the ISSCR 2014 Annual Meeting and can be combined with the Differentiation of Human ES and iPS Cells to Neural Progenitors Training Course on June 24th, 2014.
 
To register for this course, please complete your application here
Product Name Description Catalog # Size Price Quantity
iPS Cell Training Course Two-day training course on reprogramming of somatic cells to iPS cells will be held on June 22nd-23rd in Vancouver, BC, Canada. 00224 1 course Request pricing      

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Product Type: Training & education
Recommended For:
•Scientists interested in working with a standardized, serum- and feeder-free system to reprogram somatic cells to iPS cells
•Scientists interested in maintaining iPS cells in serum- and feeder-free culture media such as mTeSR™1 and TeSR™-E8™
•Scientists interested in gaining familiarity with isolating CD34+  cells from peripheral blood
Components:
 LECTURE TOPICS
•Isolation, selection, and expansion of blood and fibroblast cells prior to reprogramming
•Reprogramming somatic cells into iPS cells
•Feeder-free maintenance of iPS cell cultures using mTeSR™1 or TeSR™-E8™ media, and Matrigel™ or Vitronectin XF™ matrices
•Characterization of iPS cell colonies
•Methods to generate embryoid bodies for differentiation, including AggreWell™
 
PRACTICAL LABORATORY SESSIONS
•Isolate peripheral blood mononuclear cells with SepMate™ and enrich CD34+ cells using EasySep™
•Transfection of somatic cells with reprogramming factors
•Observe morphological changes during the reprogramming process using TeSR™-E7™
•Identify and mechanically select fully reprogrammed iPS cell colonies
•Passage ES and iPS cell cultures in mTeSR™1 & Matrigel™ or Vitronectin XF™ & TeSR™-E8™ using Gentle Cell Dissociation Reagent
•Remove differentiated areas during passaging
•Cryopreserve and thaw iPS cells
•Create uniformly-sized embryoid bodies using AggreWell™
 
Contains:
REGISTRATION INCLUDES
•Dinner with STEMCELL Technologies' experts
•Lunches and refreshments served daily
•Course booklet and electronic versions of instructional material
•Certificate issued upon successful completion of the training course
•Post laboratory Q&A and quiz on second day
 
WHERE
STEMCELL Technologies
1618 Station Street
Vancouver, BC, Canada
V6A 1B6
Area of Interest: Embryonic stem cell & iPS cell research
Cell Type: Embryonic stem cells & iPS cells
Training & Education Type: Training courses

Product Name

Description

Catalog #

Differentiation of Human ES and iPS Cells to Neural Progenitor Cells Training Course Differentiation of Human ES Cells and iPS Cells to Neural Progenitor Cells Training Course 00219
mTeSR™1 Defined, Feeder-Free Maintenance Medium for Human ES and iPS Cells 05850
TeSR™-E7™ Feeder-Free, Xeno-Free Reprogramming Medium for Human iPS Cell Induction 05910
Vitronectin XF™ Chemically Defined, Xeno-Free Cell Attachment Factor that Supports the Growth and Differentiation of Human Pluripotent Stem Cells Under Serum-Free, Feeder-Free Conditions 07180
AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
CryoStor® CS10 Animal Component-Free, Defined Cryopreservation Medium 07930
STEMdiff™ Neural Rosette Selection Reagent Enzyme-Free Reagent for the Selective Detachment of Neural Rosette Structures 05832
STEMdiff™ Neural Progenitor Medium For Maintenance and Expansion of Neural Progenitor Cells Derived from Human ES and iPS Cells 05833
STEMdiff™ Neural Progenitor Freezing Medium For Cryopreservation of Neural Progenitor Cells Generated Using STEMdiff™ Neural Induction Medium 05838
STEMdiff™ Definitive Endoderm Kit Fully Defined Animal Component Free Medium for the Differentiation of Human ES and iPS Cells to Definitive Endoderm. 05110

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