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Recommended for:
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• Increasing confidence in colony identification and enumeration
• Developing an understanding of assay theory and experimental design
• Increasing proficiency in assay set-up
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Components:
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Theory and Applications
• Overview of hematopoiesis
• Detection and quantitation of progenitors in cord blood and bone marrow
• Applications of hematopoietic assays
• Introduction to colony identification
• Technical tips and standardization of the CFC assay
• Selecting the appropriate media formulation
• Using samples from different species
• Opportunity for questions and discussion with instructors
Practical Laboratory Session
• Processing cells for the CFC assay
• Differences in processing fresh and frozen samples
• Setting up the CFC assay using MethoCult™
• Identification of hematopoietic colonies derived from CFCs
• Comparative counting exercise with instructors and course participants
• Maintaining incubators for optimal cultures
• Opportunity to revisit laboratory sessions for extra practice
• Introduction to STEMVision™, the new instrument for automated and standardized CFC enumeration
Registration Includes
• Dinner at an award-winning west coast restaurant
• Refreshments
• Lunch
• Instructional materials
• Certificate issued upon succesful completion of the training course
Where
STEMCELL Technologies
570 West Seventh Avenue
Vancouver, BC, Canada
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Intended Use Statement:
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For Research Use Only. Not for Therapeutic or Diagnostic Use.
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