•Scientists interested in deriving iPS cell lines for use in neuroscience research.
•Scientists interested in maintaining iPS cells in serum- and feeder-free culture media such as mTeSR™1 and TeSR™-E8™.
•Scientists interested in differentiating ES cells and iPS cells into neural progenitors.
•Isolation, selection, and expansion of blood and fibroblast cells prior to reprogramming
•Reprogramming somatic cells into iPS cells
•Feeder-free maintenance of iPS cell cultures using mTeSR™1 or TeSR™-E8™ media, and Matrigel™ or Vitronectin XF™ matrices
•Detailed presentation on in vitro differentiation of ES and iPS cells into the neural lineage using the STEMdiff™ neural system.
•Overview of methods to differentiate neural progenitor cells into various neural lineage-committed cells such as different types of neurons, astrocytes and oligodendrocytes.
PRACTICAL LABORATORY SESSIONS
•Reprogramming somatic cells to iPS cells
•Culture of ES and iPS cell cultures in mTeSR™1
•Formation of uniformly sized embryoid bodies using AggreWell™
•Differentiation of ES and iPS Cells to neural progenitors using monolayer- and embryoid body-based protocols with STEMdiff™ Neural Induction Medium
•Assessment of neural differentiation efficiency
•Identification and selection of CNS-type neural rosettes using STEMdiff™ Neural Rosette Selection Reagent
•Expansion of neural progenitor cells using STEMdiff™ Neural Progenitor Expansion Medium
•Dinner with STEMCELL Technologies' experts
•Lunches and refreshments served daily
•Course booklet and electronic versions of instructional material
•Certificate issued upon successful completion of the training course
•Post laboratory Q&A and quiz on second day
1618 Station Street
Vancouver, BC, Canada