Differentiation of Human ES and iPS Cells to Neural Progenitor Cells Training Course

? 1.800.667.0322   > Contact Us

Differentiation of Human ES Cells and iPS Cells to Neural Progenitor Cells Training Course

  • Differentiation of Human ES Cells and iPS cells to Neural Progenitors Training Course
Differentiation of Human ES Cells and iPS cells to Neural Progenitors Training Course
This two-day course is focused on the techniques required to efficiently and effectively differentiate human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells to neural progenitors using a defined, feeder- and enzyme-free culture system. The entire workflow from maintaining ES and iPS cells using a defined, feeder-independent culture system to obtaining pure neural progenitor cells will be covered. Participants will gain all of the skills required to begin adopting pluripotent stem cell models for neuroscience research.  Over half of the course will be devoted to hands-on laboratory techniques led by expert STEMCELL Technologies trainers with the main focus on the differentiation, identification and selection of neural progenitors.
 
To register for this course, please complete your application here
Product Name Description Catalog # Size Price Quantity
Differentiation of Human ES and iPS Cells to Neural Progenitors Training Course Two day course on the differentiation of human ES cells and iPS cells to neural progenitors will be held in Leiden, Netherlands on December 2nd-3rd, 2014 00219 1 course Request pricing      

Order Offline:

Call Us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email Us:
 
Fax Us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Product Type: Training & education
Recommended For:
• Scientists interested in maintaining human ES and iPS cells in serum- and feeder-free culture media such as mTeSR™1
• Scientists interested in differentiating ES cells and iPS cells into neural progenitors
Components:
LECTURE TOPICS
• Feeder-free maintenance of iPS cell cultures using mTeSR™1 or TeSR™-E8™ media, and Matrigel™ or Vitronectin XF™ matrices.
• Detailed presentation on in vitro differentiation of ES and iPS cells into the neural lineage using the STEMdiff™ neural system
• Overview of methods to differentiate neural progenitor cells into various neural lineage-committed cells such as different types of neurons, astrocytes and oligodendrocytes
 
PRACTICAL LABORATORY SESSIONS
• Culture of human ES and iPS cell cultures in mTeSR™1
• Formation of uniformly sized embryoid bodies (EBs) using AggreWell™
• Differentiation of ES and iPS Cells to neural progenitors using monolayer- and EB-based protocols with STEMdiff™ Neural Induction Medium
• Assessment of neural differentiation efficiency
• Identification and selection of neural rosettes using STEMdiff™ Neural Rosette Selection Reagent
• Expansion of neural progenitor cells using STEMdiff™ Neural Progenitor Expansion Medium
Contains:
REGISTRATION INCLUDES
• Dinner with STEMCELL Technologies' experts
• Lunches and refreshments served daily
• Course booklet and electronic versions of instructional material
• Post laboratory Q&A session and quiz on second day
• Certificate issued upon successful completion of the training course
 
WHERE
Leiden University Medical Center
Albinusdreef 2, 2333 ZA
Leiden, Netherlands
Area of Interest: Embryonic stem cell & iPS cell research
Cell Type: Embryonic stem cells & iPS cells, Neural stem & progenitor cells
Training & Education Type: Training courses

Protocols and Manuals

Current documents
Product NameLot# ?Document TypeDownload
Differentiation of Human ES and iPS Cells to Neural Progenitor Cells Training Course All Manual
Differentiation of Human ES and iPS Cells to Neural Progenitor Cells Training Course All Manual
 

Educational resources:

 

Product Name

Description

Catalog #

STEMdiff™ Neural Induction Medium Defined, Serum-Free Induction Medium for Neural Differentiation of Human ES and iPS Cells 05835
STEMdiff™ Neural Progenitor Medium For Maintenance and Expansion of Neural Progenitor Cells Derived from Human ES and iPS Cells 05833
STEMdiff™ Neural Rosette Selection Reagent Enzyme-Free Reagent for the Selective Detachment of Neural Rosette Structures 05832
AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
mTeSR™1 Defined, Feeder-Free Maintenance Medium for Human ES and iPS Cells 05850
TeSR™2 Animal protein-free, defined, feeder-independent medium for maintenance of undifferentiated human ES and iPS cells 05860
TeSR™-E8™ Feeder-Free, Xeno-Free Culture Medium for Maintenance of Human ES Cells and iPS Cells 05940
ACCUTASE™ ACCUTASE™ Cell Detachment Solution 07920
DMEM/F-12 with 15 mM HEPES Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12), with 15 mM HEPES Buffer 36254
D-PBS (without Ca++ and Mg++) Dulbecco’s Phosphate Buffered Saline (D-PBS) Without Calcium and Magnesium 37350

Recently Viewed Items