Differentiation of Human ES and iPS Cells to Neural Progenitor Cells Training Course

? 1.800.667.0322   > Contact Us

Differentiation of Human ES Cells and iPS Cells to Neural Progenitor Cells Training Course

  • Differentiation of Human ES Cells and iPS cells to Neural Progenitors Training Course
Differentiation of Human ES Cells and iPS cells to Neural Progenitors Training Course
This three-day course is focused on the techniques required to efficiently and effectively differentiate embryonic stem cells and induced pluripotent stem cells (ES and iPS cells) to neural progenitors using a defined, feeder- and enzyme-free culture system. Derivation of iPS cells and maintenance of human ES and iPS cells will also be taught, to ensure that participants acquire all of the skills required to begin adopting pluripotent stem cell models for neuroscience research.  Over half of the course will be devoted to hands-on laboratory techniques led by expert scientists and educators from STEMCELL Technologies with the main focus on the differentiation, identification and selection of neural progenitors.
 
Note: This course, hosted in Vancouver following the ISSCR 2014 Annual Meeting, is an extended three-day course that features one day on reprogramming, one on maintenance and one day on neural differentiation.
 
To register for this course, please complete your application here.
Product Name Description Catalog # Size Price Quantity
Differentiation of Human ES and iPS Cells to Neural Progenitors Training Course Three day course on the differentiation of human ES cells and iPS cells to neural progenitors will be held on June 22nd-24th in Vancouver, BC, Canada. 00219 1 course Request pricing      

Order Offline:

Call Us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email Us:
 
Fax Us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Product Type: Training & education
Recommended For:
•Scientists interested in deriving iPS cell lines for use in neuroscience research.
•Scientists interested in maintaining iPS cells in serum- and feeder-free culture media such as mTeSR™1 and TeSR™-E8™.
•Scientists interested in differentiating ES cells and iPS cells into neural progenitors.
Components:
LECTURE TOPICS
•Isolation, selection, and expansion of blood and fibroblast cells prior to reprogramming
•Reprogramming somatic cells into iPS cells
•Feeder-free maintenance of iPS cell cultures using mTeSR™1 or TeSR™-E8™ media, and Matrigel™ or Vitronectin XF™ matrices
•Detailed presentation on in vitro differentiation of ES and iPS cells into the neural lineage using the STEMdiff™ neural system.
•Overview of methods to differentiate neural progenitor cells into various neural lineage-committed cells such as different types of neurons, astrocytes and oligodendrocytes.
 
PRACTICAL LABORATORY SESSIONS
•Reprogramming somatic cells to iPS cells
•Culture of ES and iPS cell cultures in mTeSR™1
•Formation of uniformly sized embryoid bodies using AggreWell™
•Differentiation of ES and iPS Cells to neural progenitors using monolayer- and embryoid body-based protocols with STEMdiff™ Neural Induction Medium
•Assessment of neural differentiation efficiency
•Identification and selection of CNS-type neural rosettes using STEMdiff™ Neural Rosette Selection Reagent
•Expansion of neural progenitor cells using STEMdiff™ Neural Progenitor Expansion Medium
Contains:
REGISTRATION INCLUDES
•Dinner with STEMCELL Technologies' experts
•Lunches and refreshments served daily
•Course booklet and electronic versions of instructional material
•Certificate issued upon successful completion of the training course
•Post laboratory Q&A and quiz on second day
 
WHERE
STEMCELL Technologies
1618 Station Street
Vancouver, BC, Canada
V6A 1B6
Area of Interest: Embryonic stem cell & iPS cell research
Cell Type: Embryonic stem cells & iPS cells, Neural stem & progenitor cells
Training & Education Type: Training courses

Product Name

Description

Catalog #

STEMdiff™ Neural Induction Medium Defined, Serum-Free Induction Medium for Neural Differentiation of Human ES and iPS Cells 05835
STEMdiff™ Neural Progenitor Medium For Maintenance and Expansion of Neural Progenitor Cells Derived from Human ES and iPS Cells 05833
STEMdiff™ Neural Rosette Selection Reagent Enzyme-Free Reagent for the Selective Detachment of Neural Rosette Structures 05832
AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
mTeSR™1 Defined, Feeder-Free Maintenance Medium for Human ES and iPS Cells 05850
TeSR™2 Animal protein-free, defined, feeder-independent medium for maintenance of undifferentiated human ES and iPS cells 05860
TeSR™-E8™ Feeder-Free, Xeno-Free Culture Medium for Maintenance of Human ES Cells and iPS Cells 05940
ACCUTASE™ ACCUTASE™ Cell Detachment Solution 07920
DMEM/F-12 with 15 mM HEPES Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12), with 15 mM HEPES Buffer 36254
D-PBS (without Ca++ and Mg++) Dulbecco’s Phosphate Buffered Saline (D-PBS) Without Calcium and Magnesium 37350

Recently Viewed Items