EpiCult®-B Medium Kit (Human)

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Kit to Culture and Evaluate Human Mammary Epithelial Cells

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  • EpiCult®-B Human Medium Kit
  • Label for EpiCult®-B Basal Medium 100 mL
  • Vial label for EpiCult®-B Supplement 1 mL
EpiCult®-B Human Medium Kit
This is a kit containing serum-free liquid culture medium, optimized for the culture of human mammary luminal and myoepithelial cells. It is ideal for the growth and evaluation of bipotent, luminal-restricted and myoepithelial-restricted mammary epithelial progenitors in the mammary colony-forming cell assay when used in conjunction with an irradiated feeder layer such as NIH 3T3. This medium is also ideal for enzymatic dissociation of human mammary tissue when supplemented with collagenase and hyaluronidase. This kit contains EpiCult®-B Human Basal Medium (Catalog #05602) and EpiCult®-B Human Proliferation Supplement (Catalog #05603). Addition of freshly dissolved Hydrocortisone (Catalog #07904) is required.
Product Name Description Catalog # Size Price Quantity
EpiCult®-B Medium Kit (Human) Kit to culture and evaluate human mammary epithelial cells 05601 1 Kit 140.00 USD      
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Recommended for:
Detection and culture of human mammary epithelial progenitor cells in mammary colony forming cell assays derived from primary human breast tissues
Components:
• EpiCult® Basal Medium (Human), 100mL (Catalog #05602)
• EpiCult® Proliferation Supplement (Human), 1mL (Catalog #05603)
Accessory Products:
• Ammonium Chloride Solution (Catalog #07800)
• Collagen Solution (Bovine) (Catalog #04902)
• Collagenase/Hyaluronidase Solution (10X) (Catalog #07912)
• Dispase (5 mg/mL) (Catalog #07913)
• DNase I (Catalog #07900)
• Fetal Bovine Serum (Catalog #06100 or quality cell culture-tested equivalent)
• L-glutamine (Catalgo #07100)
• Hanks' Balanced Salt Solution Modified (Catalog #37150)
• Hydrocortisone (Catalog #07904)
• Tissue Dissociation Flask (Catalog #27300)
• Trypsin-EDTA (0.25%) (Catalog #07901)
• 40 µm Cell Strainers (Catalog #27305)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
NA
Equipment Required:
none
Legal Statement: none
Product Type: Specialized cell culture media
Application: Colony assays & quantification, Differentiation
Area of Interest: Cancer, Mammary cell research, Stem cell biology
Cell Type: Mammary epithelial cells
Medium Type: Liquid, Serum-free
Popular Product Line: EpiCult
Species: Human

This product has been used in:

  1. Maximilian Diehn et al. Association of reactive oxygen species levels and radioresistance in cancer stem cells.Nature 458 (7239) 780-783 (February 4, 2009)
  2. Peter Eirew et al. A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability.Nat Med 14 (12) 1384-1389 (December 2008)
  3. Afshin Raouf et al. Transcriptome analysis of the normal human mammary cell commitment and differentiation process.Cell Stem Cell 3 (1) 109-118 (July 3, 2008)
  4. Charlotta Lindvall et al. The Wnt signaling receptor Lrp5 is required for mammary ductal stem cell activity and Wnt1-induced tumorigenesis.J Biol Chem 281 (46) 35081-35087 (November 17, 2006)
  5. J Stingl et al. Characterization of bipotent mammary epithelial progenitor cells in normal adult human breast tissue.Breast Cancer Res Treat 67 (2) 93-109 (May 2001)

Background References:

  1. Mark Shackleton et al. Generation of a functional mammary gland from a single stem cell.Nature 439 (7072) 84-88 (January 5, 2006)
  2. E C Kordon et al. An entire functional mammary gland may comprise the progeny from a single cell.Development 125 (10) 1921-1930 (May 1998)
  3. G H Smith et al. Experimental mammary epithelial morphogenesis in an in vivo model: evidence for distinct cellular progenitors of the ductal and lobular phenotype.Breast Cancer Res Treat 39 (1) 21-31 (1996)

Product Name

Description

Catalog #
EpiCult®-C Human Medium Kit Medium for Human Mammary Epithelial Cell Culture 05630
MammoCult® Human Medium Kit Medium for the Culture of Human Mammospheres and Tumorspheres 05620
EpiCult®-B Mouse Medium Kit Medium for Mouse Mammary Epithelial Cell Culture 05610
Collagenase/Hyaluronidase 10X Collagenase/Hyaluronidase in DMEM 07912
Dispase (5 mg/mL) 5 mg/mL Dispase in Hank's Balanced Salt Solution Modified 07913
DNase I Solution (1 mg/mL) Deoxyribonuclease I Solution (1 mg/mL) 07900
Trypsin-EDTA (0.25%) Trypsin-EDTA (0.25%) 07901
Hydrocortisone Hydrocortisone 07904
40 µm Cell Strainer 40 µm Nylon Cell Strainer 27305
HBSS with 10mM HEPES, w/o Phenol Red Hanks' Balanced Salt Solution Modified (HBSS) with 10mM Hepes, Without Phenol Red 37150
Fetal Bovine Serum for Human Myeloid Colony-Forming Cells For Preparation of Methylcellulose-Based Medium for Human Myeloid Colony-forming Cells 06100
EasySep® Human MUC1 Positive Selection Kit Immunomagnetic Positive Selection Kit 18359
EasySep® Human EpCAM Positive Selection Kit Immunomagnetic Positive Selection Kit 18356
EasySep® Human CD10 Positive Selection Kit Immunomagnetic Positive Selection Kit 18358
Mammary Stem Cell Training Course Training Course for Mammary Stem Cells 00212

Protocol for isolation and identification of human and mouse mammary epithelial progenitor cells


Protocol for isolation and identification of human and mouse mammary progenitor epithelial cells

Phase contrast photographs of (A) a pure human myoepithelial cell colony, (B) a pure human luminal cell colony, and (C) a mixed human colony. (D) is a mouse colony. Unlike human mammary CFC colonies, subtypes of mouse mammary epithelial cell colonies are not easily identifiable. All colonies were cultured in either EpiCult®-B (Human: Catalog #05601) or EpiCult®-B (Mouse:Catalog #5610) in the presence of an irradiated NIH 3T3 feeder layer. Colonies were visualized by staining with Wright's Giemsa. (E) is a picture of mammospheres obtained from primary human mammary epithelial cells and (F) is an image of tumorspheres obtained from MCF7 human breast cancer cell line.



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