Hybridoma generation & transfected cell selection

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Traditional methods for selection and cloning are often regarded as the main bottleneck in the development of cell lines for antibody and recombinant protein production. ClonaCell^®’s methylcellulose-based semi-solid media and its automated platform, the ClonaCell^® EasyPick, address these limitations by offering quality, speed, and throughput in the generation of cell lines.

Custom formulations of ClonaCell^® are available to meet individual needs for the selection and cloning of mammalian cells.

Hybridoma generation

The traditional method of selecting and cloning antibody-producing hybridomas is performed in liquid culture and requires several limiting dilutions and screening steps to arrive at the desired antibody-producing clone. ClonaCell^®-HY is a methylcellulose-based semi-solid selective medium that combines HAT selection and cloning in one step.

Transfected cell selection

Traditional procedures for generating transfected cell lines using CHO or other cell lines are time-consuming and often require tedious methods to ensure that the cell population is clonal. In addition, selection in bulk liquid culture often leads to a mixture of slow and fast growing cells some of which may be non-producing. These problems can be overcome by simultaneously selecting and cloning stable transfectants using ClonaCell^®-TCS semi-solid medium.

19 products available

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Product Name	Description	Catalog #	Size	Price
ClonaCell® EasyPick	Robotic colony picking and liquid handling instrument	30000	1 Unit	Request pricing
ClonaCell®-CHO ACF Medium	Transfected CHO cell selection and cloning medium	03816	90 mL	415.00 USD
ClonaCell®-CHO CD Liquid Medium	Chemically-defined, serum-free, protein-free, animal component-free liquid medium for culture of CHO cells	03817	500 mL	103.00 USD
ClonaCell®-CHO CD Medium	Transfected CHO cell selection and cloning medium	03815	90 mL	415.00 USD
ClonaCell®-HY Hybridoma Kit	Complete kit for mouse hybridoma generation	03800	1 Kit	823.00 USD
ClonaCell®-HY Liquid HAT Selection Medium	Hybridoma selection medium containing HAT	03831	500 mL	207.00 USD
ClonaCell®-HY Medium A	Pre-fusion myeloma growth medium and hybridoma expansion medium	03801	500 mL	109.00 USD
ClonaCell®-HY Medium B	Hybridoma Fusion medium	03802	500 mL	59.00 USD
ClonaCell®-HY Medium C	Fusion recovery medium	03803	100 mL	106.00 USD
ClonaCell®-HY Medium D	Methylcellulose-based hybridoma selection and cloning medium	03804	90 mL	392.00 USD