Complete Kit for Human CD4+CD127lowCD25+ Regulatory T Cells

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Immunomagnetic Positive Selection Kit

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  • EasySep and RosetteSep Boxes
EasySep and RosetteSep Boxes
The Complete Kit for Human CD4+CD127lowCD25+ Regulatory T Cells is a simple two-step method that is designed to isolate CD4+CD127lowCD25+ cells from whole blood or buffy coat. CD4+CD127low T cells are enriched from whole blood or buffy coat by negative selection using RosetteSep® CD4+CD127low T Cell Pre-Enrichment Cocktail. The RosetteSep® cocktail contains a combination of antibodies that is directed against non-CD4+CD127low T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep® DM-L (Catalog #15705) or Ficoll-Paque™ PLUS (Catalog #07957), the unwanted cells pellet along with the RBCs. The purified cells are present as an enriched population at the interface between the plasma and the buoyant density medium. The enriched cells are then selected for CD25+ cells by positive selection using EasySep® Human CD25 Positive Selection Kit. Cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Product Name Description Catalog # Size Price Quantity
Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells Complete kit for human CD4+CD127lowCD25+ regulatory T cells 15861 For labeling 200 mL of whole blood 741.00 USD      
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RoboSep® Kit for Human CD4+CD127lowCD25+ Regulatory T Cells with Filter Tips Complete kit with RoboSep® buffer and RoboSep® filter tips 15861RF  For labeling 200 mL of whole blood 726.00 USD      
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RosetteSep® Human CD4+CD127low T Cell Enrichment Cocktail Immunodensity isolation of untouched CD4+CD127low cells for pre-enrichment of regulatory T cells 15361 For labeling 200 mL of whole blood 678.00 USD      
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Call us: +1.604.877.0713  
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Recommended for:
The isolation of human CD4+CD127lowCD25+ regulatory T cells from whole blood or buffy coat by positive selection
Components:
• RosetteSep® Human CD4+CD127low T Cell Pre-Enrichment Cocktail, 5 x 2 mL
• EasySep® Human CD25 Positive Selection Cocktail, 1 mL
• EasySep® Magnetic Particles, 1 mL
Accessory Products:
• Ficoll-Paque™ PLUS (Catalog #07957), or
• RosetteSep® DM-L (Catalog #15705)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
N/A
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• "The Big Easy" EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Legal Statement:
N/A
Product Type: Cell isolation products
Area of Interest: Immunology
Cell Isolation Product Type: Reagents
Cell Source: Buffy coat, Whole blood
Cell Type: Regulatory T cells
Popular Product Line: EasySep, RosetteSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

Product Name

Description

Catalog #
Complete Kit for Human CD4+CD25+ T Cells Immunomagnetic Positive Selection Kit 15862
Complete Kit for Human CD4+CD127lowCD49d-CD25high Regulatory T Cells Immunomagnetic Positive Selection Kit 15864
RosetteSep™ DM-L Density Medium Density Gradient Medium 15705
RosetteSep® Human CD4+ T Cell Enrichment Cocktail Immunodensity Negative Selection Cocktail 15022
EasySep® Human CD25 Positive Selection Kit Immunomagnetic Positive Selection Kit 18231
CD4 Antibody, Clone L120, PE-Conjugated Mouse Monoclonal Antibody to Human CD4 - PE Conjugated 10435
CD4 Antibody, Clone L120, FITC-Conjugated Mouse Monoclonal Antibody to Human CD4 - FITC Conjugated 10434
CD25/IL-2R-alpha Antibody, Clone 2A3, PE-Conjugated Mouse Monoclonal Antibody to Human CD25 (IL-R2α) - PE Conjugated 10512
CD25/IL-2Ra Antibody, Clone 2A3, FITC-Conjugated Mouse Monoclonal Antibody to Human CD25 (IL-2Rα) - FITC Conjugated 10412
Ficoll-Paque™ PLUS Density Gradient Medium for the Isolation of Mononuclear Cells 07907

Typical FACs Analysis of Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit:


FACs Plot
Starting with fresh whole blood, the CD4 + cell fraction is typically 75.5 – 98.6 % and 64.6 – 90.9% CD25+FoxP3+.


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