Applications of Our Matrices and Substrates
Cells in the human body are accustomed to the presence of neighboring cells, membranes, fibrous layers and adhesion proteins. This complex three-dimensional environment cannot be replicated by culturing cells in a flat, plastic flask, which results in an artificial two-dimensional layer of cells. STEMCELL Technologies’ extracellular matrices and proteins are able to host a more physiologically relevant environment allowing the progression of cell development and growth.
Matrices
Vitronectin
Recommended Uses:
Cell attachment and spreading
Features/Advantages:
- Vitronectin XF™: defined, xeno-free and compatible with the TeSR™ Family Media for maintenance of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells
- CellAdhere™ Vitronectin: ideal for coating surfaces with a thin layer of protein to support cell attachment
Collagen
Recommended Uses:
Cell attachment, orientation, migration and ECM studies
Features/Advantages:
- CellAdhere™ Type I Collagen, Bovine: use as a 3D gel
- CellAdhere™ Type I Collagen, Human: compatible with epithelial, endothelial, muscle and nerve cells
- Available in multiple formats: solution, lyophilized powder and aligned-braided or aligned-crimped slides
Fibronectin
Recommended Uses:
Cell adhesion, growth, migration and wound healing
Features/Advantages:
- Improves yield of neural cells during the final stages of mouse embryonic stem cell (mESC) in vitro differentiation into neural cell types
- Pre-screened with ES-Cult™ reagents
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Substrates
Tropoelastin
Recommended Uses:
Cell attachment and spreading
Features/Advantages:
- Allows modulation of surface elasticity to support cell growth and function
Gelatin
Recommended Uses:
Cell growth and maintenance
Features/Advantages:
- Supports maintenance of mouse embryonic stem cells (ESCs), mouse induced pluripotent stem cells (miPSCs) and mouse embryonic fibroblasts
- Pre-screened for use with ES-Cult™ product line
MesenCult™ Attachment Substrates
Recommended Uses:
Cell attachment
Features/Advantages:
- MesenCult™-SF Attachment Substrate: serum-free and optimized for use with Mesencult™-XF Medium for expansion and enumeration of human mesenchymal stem cells (MSCs)
- MesenCult™-ACF Attachment Substrate: animal component-free and optimized for use with MesenCult™-ACF Medium for expansion and enumeration of human MSCs
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MesenCult™-SF Attachment Substrate
MesenCult™-SF Attachment Substrate
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