Biotin Antibody, Clone C6D5.1.1

Biotin (Vitamin H) is commonly coupled to proteins and other molecules for use in a number of applications. Both Avidin and Streptavidin form strong non-covalent bonds with biotin in a 1:4 ratio and thus efficiently cross-link biotinylated molecules. Both Avidin and Streptavidin can however exhibit non-specific binding that can be problematic in certain applications. Cross-linking of biotinylated molecules, such as immunoglobulins, can also be achieved using anti-biotin antibodies such as clone C6D5.1.1. This approach may be preferable to eliminate the non-specific binding problems associated with streptavidin, or when a lesser degree of cross-linking is required.