mTeSR®1

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Defined, Feeder-Independent Maintenance Medium for hESCs and hiPSCs

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  • mTeSR®1 Complete Kit for hES Maintenance
  • Label for mTeSR®1 5X Supplement 100 mL
  • Label for mTeSR®1 Basal Medium 400 mL
mTeSR®1 Complete Kit for hES Maintenance
mTeSR®1 Medium is the most widely published feeder-independent maintenance medium for hESCs and hiPSCs. It is a complete, serum-free, defined formulation and does not require any further addition of growth factors. The medium is formulated for use with BD Matrigel™ hESC-qualified Matrix as a substrate.
Product Name Description Catalog # Size Price Quantity
mTeSR®1 Defined, feeder-Independent maintenance medium for human ESCs and iPSCs 05850 1 Kit 272.00 USD      
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mTeSR®1 Defined, feeder-Independent maintenance medium for hESCs and hiPSCs, 1 L kit 05857 1 L Kit 544.00 USD      
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Recommended for:
Maintenance of undifferentiated hESCs and hiPSCs in feeder-independent conditions
Components:
• mTeSR®1 Basal Medium, 400 mL (Catalog #05851)
• mTeSR®1 5X Supplement, 100 mL (Catalog #05852)
Accessory Products:
• TeSR™2 Animal Protein-Free Medium (Catalog #05860 / 05880)
• AggreWell™400 plates (Catalog #27845 / 27945)
• Oct 3/4 Antibody, Clone 40 (Catalog #01550 / 01551)
• SSEA-1 Antibody, Clone MC-480 (Catalog #01552)
• SSEA-3 Antibody, Clone MC-631 (Catalog #01553)
• SSEA-4 Antibody, Clone 813-70 (Catalog #01554)
• TRA-1-60 Antibody, Clone TRA-1-60 (Catalog #01555)
• TRA-1-81 Antibody, Clone TRA-1-81 (Catalog #01556)
• TRA-2-49 Antibody, Clone TRA-2-49/6E (Catalog #01557)
• TRA-2-54 Antibody, Clone TRA-2-54/2J (Catalog #01558)
• mFreSR® Defined Cryopreservation Medium (Catalog #05855 / 05854)
• CryoStor™CS10 Defined, Animal Protein-Free Cryopreservation Medium (Catalog #07930)
• ACCUTASE® (Catalog #07920)
• Dispase (1 mg/mL) (Catalog #07923)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
• Bovine Serum Albumin
• rh bFGF
• rh TGFβ
• Lithium Chloride
• Pipecolic acid
• GABA
Equipment Required:
N/A
Legal Statement: This product was developed under license to intellectual property owned by WiCell™ Research Institute (patent pending). This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers wishing to use the product for purposes other than research use should contact Geron Corporation’s Business Development office at (650) 473-7700 or corpdev@geron.com. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund.
Product Type: Specialized cell culture media
Application: Maintenance
Area of Interest: Embryonic stem cell & iPS cell research, Stem cell biology
Cell Type: Embryonic stem cells & iPS cells
Medium Type: Liquid
Popular Product Line: mTeSR1 and TeSR2
Species: Human

This product has been used in:

  1. Keith Task et al. Population based model of human embryonic stem cell (hESC) differentiation during endoderm induction.PLoS One 7 (3) e32975 (2012)
  2. Christophe Sandt et al. Identification of Spectral Modifications Occurring during Reprogramming of Somatic Cells.PLoS One 7 (4) e30743 (2012)
  3. Hiroyuki Hirai et al. Efficient iPS Cell Production with the MyoD Transactivation Domain in Serum-Free Culture.PLoS One 7 (3) e34149 (2012)
  4. Mykyta V Sokolov et al. Unraveling the global microRNAome responses to ionizing radiation in human embryonic stem cells.PLoS One 7 (2) e31028 (2012)
  5. Ruth Olmer et al. Suspension culture of human pluripotent stem cells in controlled, stirred bioreactors.Tissue Eng Part C Methods (April 20, 2012)
  6. Seiga Ohmine et al. Reprogrammed keratinocytes from elderly type 2 diabetes patients suppress senescence genes to acquire induced pluripotency.Aging (Albany NY) 4 (1) 60-73 (January 2012)
  7. Sabrina Lin et al. Methods for culturing mouse and human embryonic stem cells.Methods Mol Biol 690 31-56 (2011)
  8. Mahesh C Dodla et al. Differing lectin binding profiles among human embryonic stem cells and derivatives aid in the isolation of neural progenitor cells.PLoS One 6 (8) e23266 (2011)
  9. Simone C Cardoso et al. Synchrotron radiation X-ray microfluorescence reveals polarized distribution of atomic elements during differentiation of pluripotent stem cells.PLoS One 6 (12) e29244 (2011)
  10. Seiga Ohmine et al. Induced pluripotent stem cells from GMP-grade hematopoietic progenitor cells and mononuclear myeloid cells.Stem Cell Res Ther 2 (6) 46 (2011)
  11. Akira Niwa et al. A Novel Serum-Free Monolayer Culture for Orderly Hematopoietic Differentiation of Human Pluripotent Cells via Mesodermal Progenitors.PLoS One 6 (7) e22261 (2011)
  12. Jong S Rim et al. Screening for epigenetic target genes that enhance reprogramming using lentiviral-delivered shRNA.Methods Mol Biol 702 299-316 (2011)
  13. Junying Yu et al. Efficient feeder-free episomal reprogramming with small molecules.PLoS One 6 (3) e17557 (2011)
  14. Daniela F Quail et al. Low oxygen levels induce the expression of the embryonic morphogen Nodal.Mol Biol Cell 22 (24) 4809-4821 (December 2011)
  15. et al. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage.Nat Biotechnol 29 (12) 1132-1144 (November 27, 2011)
  16. Chulan Kwon et al. Notch post-translationally regulates ??-catenin protein in stem and progenitor cells.Nat Cell Biol 13 (10) 1244-1251 (October 2011)
  17. Chad Tang et al. An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells.Nat Biotechnol (August 14, 2011)
  18. Huai-En Lu et al. Selection of alkaline phosphatase-positive induced pluripotent stem cells from human amniotic fluid-derived cells by feeder-free system.Exp Cell Res 317 (13) 1895-1903 (August 1, 2011)
  19. Kristine K Freude et al. Soluble amyloid precursor protein induces rapid neural differentiation of human embryonic stem cells.J Biol Chem 286 (27) 24264-24274 (July 8, 2011)
  20. Heather K Bone et al. A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.J Cell Sci 124 (Pt 12) 1992-2000 (June 15, 2011)
  21. Toshio Miki et al. Hepatic differentiation of human embryonic stem cells is promoted by three-dimensional dynamic perfusion culture conditions.Tissue Eng Part C Methods 17 (5) 557-568 (May 2011)
  22. Prachi N Ghule et al. Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells.J Cell Physiol 226 (5) 1149-1156 (May 2011)
  23. Mohammad A Mandegar et al. Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells.Hum Mol Genet (May 24, 2011)
  24. Hua Liu et al. In vivo liver regeneration potential of human induced pluripotent stem cells from diverse origins.Sci Transl Med 3 (82) 82ra39 (May 11, 2011)
  25. Mykyta V Sokolov et al. Dynamics of the transcriptome response of cultured human embryonic stem cells to ionizing radiation exposure.Mutat Res 709-710 40-48 (May 10, 2011)
  26. Gautam Dravid et al. Dysregulated gene expression during hematopoietic differentiation from human embryonic stem cells.Mol Ther 19 (4) 768-781 (April 2011)
  27. Guang-Hui Liu et al. Recapitulation of premature ageing with iPSCs from Hutchinson-Gilford progeria syndrome.Nature 472 (7342) 221-225 (April 14, 2011)
  28. Jiaping Zhang et al. Electrically Guiding Migration of Human Induced Pluripotent Stem Cells.Stem Cell Rev (March 5, 2011)
  29. Pengzhi Yu et al. FGF2 Sustains NANOG and Switches the Outcome of BMP4-Induced Human Embryonic Stem Cell Differentiation.Cell Stem Cell 8 (3) 326-334 (March 4, 2011)
  30. Michael Duff et al. Genomewide characterization of non-polyadenylated RNAs.Genome Biology 12 (2) R16 (February 16, 2011)
  31. Kazim H Narsinh et al. Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells.J Clin Invest (February 7, 2011)
  32. Ryan Lister et al. Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells.Nature (February 2, 2011)
  33. James E Hudson et al. A defined medium and substrate for expansion of human mesenchymal stromal cell progenitors that enriches for osteo- and chondrogenic precursors.Stem Cells Dev 20 (1) 77-87 (January 2011)
  34. Xuejing Duan et al. Application of induced pluripotent stem (iPS) cells in periodontal tissue regeneration.J Cell Physiol 226 (1) 150-157 (January 2011)
  35. Masakazu Kamata et al. Live cell monitoring of hiPSC generation and differentiation using differential expression of endogenous microRNAs.PLoS One 5 (7) e11834 (2010)
  36. Karin Norrman et al. Quantitative comparison of constitutive promoters in human ES cells.PLoS One 5 (8) e12413 (2010)
  37. Matthew E Brown et al. Derivation of induced pluripotent stem cells from human peripheral blood T lymphocytes.PLoS One 5 (6) e11373 (2010)
  38. A M Fernandes et al. Worldwide survey of published procedures to culture human embryonic stem cells.Cell Transplant 19 (5) 509-523 (2010)
  39. Mykyta V Sokolov et al. Radiation-induced bystander effects in cultured human stem cells.PLoS One 5 (12) e14195 (2010)
  40. Katharina Wolfrum et al. The LARGE principle of cellular reprogramming: lost, acquired and retained gene expression in foreskin and amniotic fluid-derived human iPS cells.PLoS One 5 (10) e13703 (2010)
  41. Nil Emre et al. The ROCK Inhibitor Y-27632 Improves Recovery of Human Embryonic Stem Cells after Fluorescence-Activated Cell Sorting with Multiple Cell Surface Markers.PLoS One 5 (8) (2010)
  42. Sabrina Lin et al. Video bioinformatics analysis of human embryonic stem cell colony growth.J Vis Exp (39) (2010)
  43. Jinnuo Han et al. A combined epigenetic and non-genetic approach for reprogramming human somatic cells.PLoS One 5 (8) e12297 (2010)
  44. Wing-Hon Lai et al. ROCK inhibition facilitates the generation of human-induced pluripotent stem cells in a defined, feeder-, and serum-free system.Cell Reprogram 12 (6) 641-653 (December 2010)
  45. S Jagtap et al. Cytosine arabinoside induces ectoderm and inhibits mesoderm expression in human embryonic stem cells during multi-lineage differentiation.Br J Pharmacol (December 30, 2010)
  46. Daniela Moralli et al. An Improved Technique for Chromosomal Analysis of Human ES and iPS Cells.Stem Cell Rev (December 29, 2010)
  47. Jiayin Yang et al. Induced pluripotent stem cells can be used to model the genomic imprinting disorder Prader-Willi syndrome.J Biol Chem 285 (51) 40303-40311 (December 17, 2010)
  48. Masakazu Kamata et al. Generation of human induced pluripotent stem cells bearing an anti-HIV transgene by a lentiviral vector carrying an internal murine leukemia virus promoter.Hum Gene Ther 21 (11) 1555-1567 (November 2010)
  49. Sabrina Lin et al. Comparison of the toxicity of smoke from conventional and harm reduction cigarettes using human embryonic stem cells.Toxicol Sci 118 (1) 202-212 (November 2010)
  50. Andrew B J Prowse et al. Long term culture of human embryonic stem cells on recombinant vitronectin in ascorbate free media.Biomaterials 31 (32) 8281-8288 (November 2010)
  51. Alysson R Muotri et al. L1 retrotransposition in neurons is modulated by MeCP2.Nature 468 (7322) 443-446 (November 18, 2010)
  52. Joseph R Klim et al. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.Nat Methods (November 14, 2010)
  53. Maria C N Marchetto et al. A model for neural development and treatment of rett syndrome using human induced pluripotent stem cells.Cell 143 (4) 527-539 (November 12, 2010)
  54. Sabine Loewer et al. Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells.Nat Genet (November 7, 2010)
  55. Jiaxi Zhou et al. High-efficiency induction of neural conversion in human ESCs and human induced pluripotent stem cells with a single chemical inhibitor of transforming growth factor beta superfamily receptors.Stem Cells 28 (10) 1741-1750 (October 2010)
  56. Kyle E Hammerick et al. Elastic Properties of Induced Pluripotent Stem Cells.Tissue Eng Part A (October 25, 2010)
  57. Kitchener D Wilson et al. Dynamic MicroRNA Expression Programs During Cardiac Differentiation of Human Embryonic Stem Cells: Role for miR-499.Circ Cardiovasc Genet 3 (5) 426-435 (October 1, 2010)
  58. Ying Mei et al. Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells.Nat Mater 9 (9) 768-778 (September 2010)
  59. Tae-Min Yoon et al. Human embryonic stem cells (hESCs) cultured under distinctive feeder-free culture conditions display global gene expression patterns similar to hESCs from feeder-dependent culture conditions.Stem Cell Rev 6 (3) 425-437 (September 2010)
  60. Andrea Walker et al. Non-muscle myosin II regulates survival threshold of pluripotent stem cells.Nat Commun 1 (6) doi:10.1038/ncomms1074 (September 2010)
  61. Luigi Warren et al. Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA.Cell Stem Cell (September 29, 2010)
  62. Xiaoyan Xie et al. Effects of Long-Term Culture on Human Embryonic Stem Cell Aging.Stem Cells Dev (September 9, 2010)
  63. Ling-Ling Chen et al. Molecular basis for an attenuated cytoplasmic dsRNA response in human embryonic stem cells.Cell Cycle 9 (17) 3552-3564 (September 1, 2010)
  64. Guokai Chen et al. Actin-myosin contractility is responsible for the reduced viability of dissociated human embryonic stem cells.Cell Stem Cell 7 (2) 240-248 (August 6, 2010)
  65. Michael R Doran et al. Defined high protein content surfaces for stem cell culture.Biomaterials 31 (19) 5137-5142 (July 2010)
  66. Ruth Olmer et al. Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium.Stem Cell Res 5 (1) 51-64 (July 2010)
  67. Kristin Atze et al. Efficient, high-throughput transfection of human embryonic stem cells.Stem Cell Research & Therapy 1 (3) 23 (July 26, 2010)
  68. Vincenzo Calvanese et al. Sirtuin 1 regulation of developmental genes during differentiation of stem cells.Proc Natl Acad Sci U S A (July 14, 2010)
  69. Yuin-Han Loh et al. Reprogramming of T cells from human peripheral blood.Cell Stem Cell 7 (1) 15-19 (July 2, 2010)
  70. Kitchener D Wilson et al. Effects of ionizing radiation on self-renewal and pluripotency of human embryonic stem cells.Cancer Res 70 (13) 5539-5548 (July 1, 2010)
  71. Zara Melkoumian et al. Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells.Nat Biotechnol 28 (6) 606-610 (June 2010)
  72. Sergey Rodin et al. Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511.Nat Biotechnol 28 (6) 611-615 (June 2010)
  73. Harmeet Singh et al. Up-scaling single cell-inoculated suspension culture of human embryonic stem cells.Stem Cell Res 4 (3) 165-179 (May 2010)
  74. Jacob Hanna et al. Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs.Proc Natl Acad Sci U S A 107 (20) 9222-9227 (May 18, 2010)
  75. R David Hawkins et al. Distinct epigenomic landscapes of pluripotent and lineage-committed human cells.Cell Stem Cell 6 (5) 479-491 (May 7, 2010)
  76. Ken-ichiro Kamei et al. Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions.Lab Chip 10 (9) 1113-1119 (May 7, 2010)
  77. Mykyta V Sokolov et al. Expression of pluripotency-associated genes in the surviving fraction of cultured human embryonic stem cells is not significantly affected by ionizing radiation.Gene 455 (1-2) 8-15 (May 1, 2010)
  78. Guoliang Meng et al. Extracellular matrix isolated from foreskin fibroblasts supports long-term xeno-free human embryonic stem cell culture.Stem Cells Dev 19 (4) 547-556 (April 2010)
  79. Zara Hannoun et al. The comparison between conditioned media and serum-free media in human embryonic stem cell culture and differentiation.Cell Reprogram 12 (2) 133-140 (April 2010)
  80. Veronika Akopian et al. Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells.In vitro cellular & developmental biology. Animal 46 (3-4) 247-258 (April 2010)
  81. Jinglei Cai et al. Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells.J Biol Chem 285 (15) 11227-11234 (April 9, 2010)
  82. Shigeki Sugii et al. Human and mouse adipose-derived cells support feeder-independent induction of pluripotent stem cells.Proc Natl Acad Sci U S A 107 (8) 3558-3563 (February 23, 2010)
  83. Suneet Agarwal et al. Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients.Nature 464 (7286) 292-296 (February 17, 2010)
  84. Samira M Azarin et al. Development of Scalable Culture Systems for Human Embryonic Stem Cells.Biochem Eng J 48 (3) 378 (February 15, 2010)
  85. Fangjun Jia et al. A nonviral minicircle vector for deriving human iPS cellsNat Meth advance online publication (3) 197-199 (February 7, 2010)
  86. Ratmir Derda et al. High-throughput discovery of synthetic surfaces that support proliferation of pluripotent cells.J Am Chem Soc 132 (4) 1289-1295 (February 3, 2010)
  87. Norikatsu Miyoshi et al. Defined factors induce reprogramming of gastrointestinal cancer cells.Proc Natl Acad Sci U S A 107 (1) 40-45 (January 5, 2010)
  88. Gene Yeo et al. Transcriptional signature and memory retention of human-induced pluripotent stem cells.PLoS One 4 (9) e7076 (2009)
  89. Jin Yu et al. nAChRs mediate human embryonic stem cell-derived endothelial cells: proliferation, apoptosis, and angiogenesis.PLoS One 4 (9) e7040 (2009)
  90. Zongjin Li et al. Functional and transcriptional characterization of human embryonic stem cell-derived endothelial cells for treatment of myocardial infarction.PLoS One 4 (12) e8443 (2009)
  91. James N Hughes et al. A novel role for gamma-secretase in the formation of primitive streak-like intermediates from ES cells in culture.Stem Cells 27 (12) 2941-2951 (December 2009)
  92. Xiaofang Wang et al. Inhibition of caspase-mediated anoikis is critical for basic fibroblast growth factor-sustained culture of human pluripotent stem cells.J Biol Chem 284 (49) 34054-34064 (December 4, 2009)
  93. Elayne Chan et al. Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells.Nat Biotechnol 27 (11) 1033-1037 (November 2009)
  94. I Mateizel et al. Characterization of CD30 expression in human embryonic stem cell lines cultured in serum-free media and passaged mechanically.Hum Reprod 24 (10) 2477-2489 (October 2009)
  95. Gabriel Kolle et al. Identification of human embryonic stem cell surface markers by combined membrane-polysome translation state array analysis and immunotranscriptional profiling.Stem Cells 27 (10) 2446-2456 (October 2009)
  96. Hui Zeng et al. Lack of ABCG2 expression and side population properties in human pluripotent stem cells.Stem Cells 27 (10) 2435-2445 (October 2009)
  97. Nicholas Panetta et al. Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells.Proc Natl Acad Sci U S A 106 (37) 15720-15725 (September 15, 2009)
  98. Jaehoon Chung et al. Magnetic resonance imaging of human embryonic stem cells.Curr Protoc Stem Cell Biol Chapter 5 Unit 5A.3 (August 2009)
  99. Ling-Ling Chen et al. Altered nuclear retention of mRNAs containing inverted repeats in human embryonic stem cells: functional role of a nuclear noncoding RNA.Mol Cell 35 (4) 467-478 (August 28, 2009)
  100. Prashob Porayette et al. Differential processing of amyloid-beta precursor protein directs human embryonic stem cell proliferation and differentiation into neuronal precursor cells.J Biol Chem 284 (35) 23806-23817 (August 28, 2009)
  101. Andrew Lee et al. Effects of cell number on teratoma formation by human embryonic stem cells.Cell Cycle 8 (16) 2608-2612 (August 24, 2009)
  102. Angela Cp Chin et al. Defined and serum-free media support undifferentiated human embryonic stem cell growth.Stem Cells Dev (August 17, 2009)
  103. Sarah Eminli et al. Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells.Nat Genet 41 (9) 968-976 (August 9, 2009)
  104. Heidi Hakala et al. Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell lines.Tissue Eng Part A 15 (7) 1775-1785 (July 2009)
  105. J A Kelber et al. Blockade of Cripto binding to cell surface GRP78 inhibits oncogenic Cripto signaling via MAPK/PI3K and Smad2/3 pathways.Oncogene 28 (24) 2324-2336 (June 18, 2009)
  106. Min Lu et al. Enhanced generation of hematopoietic cells from human hepatocarcinoma cell-stimulated human embryonic and induced pluripotent stem cells.Exp Hematol 37 (8) 924-936 (May 26, 2009)
  107. Jiaxi Zhou et al. mTOR supports long-term self-renewal and suppresses mesoderm and endoderm activities of human embryonic stem cells.Proc Natl Acad Sci U S A 106 (19) 7840-7845 (May 12, 2009)
  108. Xiangyun Li et al. ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells.Hum Reprod 24 (3) 580-589 (March 2009)
  109. Steve K W Oh et al. Long-term microcarrier suspension cultures of human embryonic stem cells.Stem Cell Res (March 4, 2009)
  110. Gene E Ananiev et al. Optical mapping discerns genome wide DNA methylation profiles.BMC Mol Biol 9 68 (2008)
  111. Nicole Harb et al. The Rho-Rock-Myosin signaling axis determines cell-cell integrity of self-renewing pluripotent stem cells.PLoS ONE 3 (8) e3001 (2008)
  112. A Raya et al. Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts.Cold Spring Harb Symp Quant Biol 73 127-135 (2008)
  113. Mark E Levenstein et al. Secreted proteoglycans directly mediate human embryonic stem cell-basic fibroblast growth factor 2 interactions critical for proliferation.Stem Cells 26 (12) 3099-3107 (December 2008)
  114. Maria A Lagarkova et al. CD 30 is a marker of undifferentiated human embryonic stem cells rather than a biomarker of transformed hESCs.Cell Cycle 7 (22) 3610-3612 (November 15, 2008)
  115. Rob J Thomas et al. Automated, scalable culture of human embryonic stem cells in feeder-free conditions.Biotechnol Bioeng 102 (6) 1636-1644 (November 3, 2008)
  116. Shi-Jiang Lu et al. Robust generation of hemangioblastic progenitors from human embryonic stem cells.Regen Med 3 (5) 693-704 (September 2008)
  117. C Banuelos et al. Mouse but not human embryonic stem cells are deficient in rejoining of ionizing radiation-induced DNA double-strand breaks.DNA repair 7 (9) 1471-1483 (September 1, 2008)
  118. Recombinant Vitronectin Is a Functionally Defined Substrate That Supports Human Embryonic Stem Cell Self-Renewal via {alpha}V{beta}5 IntegrinStem Cells 26 (9) 2257-2265 (September 1, 2008)
  119. Rutger-Jan Swijnenburg et al. Immunosuppressive therapy mitigates immunological rejection of human embryonic stem cell xenografts.Proc Natl Acad Sci U S A 105 (35) (August 26, 2008)
  120. Ren-He Xu et al. NANOG is a direct target of TGFbeta/activin-mediated SMAD signaling in human ESCs.Cell Stem Cell 3 (2) 196-206 (August 7, 2008)
  121. Doug Phanstiel et al. Mass spectrometry identifies and quantifies 74 unique histone H4 isoforms in differentiating human embryonic stem cellsProceedings of the National Academy of Sciences 105 (11) 4093-4098 (March 18, 2008)
  122. Michael D O'Connor et al. Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells.Stem Cells 26 (5) 1109-1116 (February 14, 2008)
  123. Nathan Blow et al. Stem cells: in search of common ground.Nature 451 (7180) 855-858 (February 14, 2008)
  124. Myon-Hee Lee et al. Conserved regulation of MAP kinase expression by PUF RNA-binding proteins.PLoS Genet 3 (12) e233 (December 28, 2007)
  125. In-Hyun Park et al. Reprogramming of human somatic cells to pluripotency with defined factors.Nature 451 (7175) 141-146 (December 23, 2007)
  126. Prashob Porayette et al. Amyloid-beta precursor protein expression and modulation in human embryonic stem cells: a novel role for human chorionic gonadotropin.Biochem Biophys Res Commun 364 (3) 522-527 (December 21, 2007)
  127. Junying Yu et al. Induced pluripotent stem cell lines derived from human somatic cells.Science 318 (5858) 1917-1920 (December 21, 2007)
  128. Hideki Masaki et al. Heterogeneity of pluripotent marker gene expression in colonies generated in human iPS cell induction culture.Stem Cell Res 1 (2) 105-115 (November 2007)
  129. Kazutoshi Takahashi et al. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors.Cell 131 (5) 861-872 (November 20, 2007)
  130. Tenneille Ludwig et al. Feeder-independent culture of human embryonic stem cells.Nat Methods 3 (8) 637-646 (August 2006)
  131. Tenneille Ludwig et al. Derivation of human embryonic stem cells in defined conditions.Nat Biotechnol 24 (2) 185-187 (March 2006)
  132. Jonathan S Draper et al. Surface antigens of human embryonic stem cells: changes upon differentiation in culture.J Anat 200 (Pt 3) 249-258 (March 2002)
  133. J A Thomson et al. Embryonic stem cell lines derived from human blastocysts.Science 282 (5391) 1145-1147 (November 6, 1998)
  134. Xia Xu et al. The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells.Biotechnol Prog 26 (3) 827-837
  135. A V Eremeev et al. Derivation of a novel human embryonic stem cell line under serum-free and feeder-free conditions.Dokl Biol Sci 426 293-295

Background References:

  1. Nathan Blow et al. Stem cells: in search of common ground.Nature 451 (7180) 855-858 (February 14, 2008)
  2. In-Hyun Park et al. Reprogramming of human somatic cells to pluripotency with defined factors.Nature 451 (7175) 141-146 (December 23, 2007)
  3. Kazutoshi Takahashi et al. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors.Cell 131 (5) 861-872 (November 20, 2007)
  4. Jonathan S Draper et al. Surface antigens of human embryonic stem cells: changes upon differentiation in culture.J Anat 200 (Pt 3) 249-258 (March 2002)
  5. J A Thomson et al. Embryonic stem cell lines derived from human blastocysts.Science 282 (5391) 1145-1147 (November 6, 1998)

Product Name

Description

Catalog #
TeSR™2 Animal Protein-Free, Defined, Feeder-independent Medium for Maintenance of Undifferentiated hESCs or hiPSCs. 05860
AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
mFreSR® Defined, Serum-Free Cryopreservation Medium for hESCs and hiPSCs 05854
CryoStor™CS10 Animal Protein-Free, Defined Cryopreservation Medium 07930
EasySep® hESC-Derived CD34 Positive Selection Kit Immunomagnetic Positive Selection Kit 18167
Oct-3/4 Antibody, Clone 40 Mouse Monoclonal Antibody to Oct-3/4 01550
SSEA-1 Antibody, Clone MC-480 Mouse Monoclonal Antibody to SSEA-1 01552
SSEA-3 Antibody, Clone MC-631 Rat Monoclonal Antibody to SSEA-3 01553
SSEA-4 Antibody, Clone 813-70 Mouse Monoclonal Antibody to Anti-SSEA-4 01554
TRA-1-81 Antibody, Clone TRA-1-81 Mouse Monoclonal Antibody to TRA-1-81 01556
Mouse IgG (H+L) Antibody, FITC-Conjugated Goat Polyclonal Antibody to Mouse IgG (H+L) - FITC Conjugated 10210
IgM Antibody, FITC-Conjugated Goat Polyclonal Antibody to Mouse IgM - FITC Conjugated 10211
IgM Antibody, APC-Conjugated Goat Polyclonal Antibody to Rat IgM - APC Conjugated 10215
Y-27632 Rho-Associated Coil Kinase (ROCK) Inhibitor 07171
ACCUTASE™ ACCUTASE™ Cell Detachment Solution 07920
Dispase (1 mg/mL) Dispase 1 mg/mL 07923

mTeSR®1 cultures show consistent expansion


mTeSR®1 cultures show consistent expansion
H1 and H9 hESCs were expanded in mTeSR®1 for 19 and 18 passages respectively. Cultures show consistent expansion at each passage.


Morphology of hESCs and hiPSCs cultured in mTeSR®1


Morphology of hESCs and hiPSCs cultured in mTeSR®1
H1 hESCs grow as colonies with (A) defined edges and (B) high nucleus to cytoplasm ratio. hiPSC lines ( C) iPSC(IMR90)-3 and (D) MSC-iPSC1 maintained in mTeSR®1 show similar morphological characteristics. hiPSC photographs courtesy of M. O'Connor and C. Eaves, The Vancouver Human Embryonic Stem Cell Core Facility


Human embryonic stem cells cultured in mTeSR®1 retain normal karyotype following long-term passage


Human embryonic stem cells cultured in mTeSR®1 retain normal karyotype following long-term passage
Chromosomal analysis of H1 hESCs cultured in mTeSR®1 for 48 passages shows that normal karyotype is retained during long-term passaging. Data from Dr. T Ludwig, WiCell Research Institute


Human embryonic stem cell cultures in mTeSR®1 are pluripotent


Human embryonic stem cell cultures in mTeSR®1 are pluripotent
H9 hESCs were cultured for 6 passages in mTeSR®1 then injected subcutaneously into immunocompromised mice. The resulting teratoma contained cell types from all 3 germ layers. Representative tissue types are shown.


Human embryonic stem cells cultured in mTeSR®1 express high levels of pluripotent markers and low levels of differentiation markers


Human embryonic stem cells cultured in either mTeSR®1 or TeSR™2 express high levels of pluripotent markers and low levels of differentiation markers
Flow cytometric analysis of H9 hESCs maintained in mTeSR®1 for 17 passages.


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