StemSpan® SFEM

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Serum-Free Medium for Expansion of Hematopoietic Cells

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  • StemSpan® SFEM 100 mL
  • StemSpan® SFEM 500 mL
  • Label for StemSpan® SFEM 100 mL
  • Label for StemSpan® SFEM 500 mL
StemSpan® SFEM 100 mL
StemSpan® Serum-Free Expansion Medium (SFEM) has been developed for the in vitro culture and expansion of human hematopoietic cells. This medium contains pre-tested bovine serum albumin, insulin and transferrin, and supplements in Iscove’s MDM. Recombinant hematopoietic growth factors, required for the optimal growth and expansion of hematopoietic cells, have not been added to StemSpan® SFEM. This allows users the flexibility to prepare medium that meets their requirements. In addition to the culture and expansion of human hematopoietic cells, StemSpan® SFEM has been used for the culture and expansion of hematopoietic stem and progenitor cells from other species, including mouse, non-human primate and dog. StemSpan® SFEM has also been used for the generation, culture and assay of human and mouse dendritic cells, and for the culture of various other hematopoietic and non-hematopoietic cell types. In most applications, addition of specific hematopoietic growth factors, cytokines and/or other compounds is required for optimal growth.
Product Name Description Catalog # Size Price Quantity
StemSpan® SFEM Serum-free medium for expansion of hematopoietic cells 09650 500 mL 304.00 USD      
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StemSpan® SFEM Serum-free medium for expansion of hematopoietic cells 09600 100 mL 89.00 USD      
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Recommended for:
• Culture and expansion of human hematopoietic progenitors in samples of bone marrow, cord blood and mobilized peripheral blood
• Expansion of mouse colony-forming cells, long-term culture-initiating cell (LTC-IC) and long-term repopulating cells
• Generation and culture of human and mouse dendritic cells
• Culture of rat and non-human primate cells
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
• Bovine Serum Albumin
• Recombinant Human Insulin
• Human Transferrin (Iron-Saturated)
• 2-Mercaptoethanol
• Iscove’s MDM
• Supplements
Product Type: Specialized cell culture media
Application: Expansion
Area of Interest: Cancer, Cord blood banking, Hematologic malignancies, Hematopoietic stem cell research, Immunology, Stem cell biology, Transplantation
Cell Type: Dendritic cells, Hematopoietic stem & progenitor cells, Monocytes
Medium Type: Liquid, Serum-free
Popular Product Line: StemSpan
Species: Human, Mouse

Procedures and instruction manuals:

Educational resources:

MSDS:

This product has been used in:

  1. Liang Li et al. A critical role for SHP2 in STAT5 activation and growth factor mediated proliferation, survival and differentiation of human CD34+ cells.Blood (June 13, 2011)
  2. Xin Shi et al. Thymopoietic and bone marrow response to murine pneumocystis pneumonia.Infect Immun 79 (5) 2031-2042 (May 2011)
  3. Jizhong Zou et al. Oxidase-deficient neutrophils from X-linked chronic granulomatous disease iPS cells: functional correction by zinc finger nuclease-mediated safe harbor targeting.Blood 117 (21) 5561-5572 (May 26, 2011)
  4. Adeel Hasan et al. The Matricellular Protein Cysteine-rich Protein 61 (CCN1/Cyr61) Enhances Physiological Adaptation of Retinal Vessels and Reduces Pathological Neovascularization Associated with Ischemic Retinopathy.J Biol Chem 286 (11) 9542-9554 (March 18, 2011)
  5. Li Zhou et al. Reduced SMAD7 leads to overactivation of TGF-beta signaling in MDS that can be reversed by a specific inhibitor of TGF-beta receptor I kinase.Cancer Res 71 (3) 955-963 (February 1, 2011)
  6. Maria E Figueroa et al. Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.Cancer Cell 18 (6) 553-567 (December 14, 2010)
  7. Emile van den Akker et al. The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.Haematologica 95 (9) 1594-1598 (September 2010)
  8. Albert S Mellick et al. Using the transcription factor inhibitor of DNA binding 1 to selectively target endothelial progenitor cells offers novel strategies to inhibit tumor angiogenesis and growth.Cancer Res 70 (18) 7273-7282 (September 15, 2010)
  9. Jie Jiang et al. Crucial roles for protein kinase C isoforms in tumor-specific killing by apoptin.Cancer Res 70 (18) 7242-7252 (September 15, 2010)
  10. Gerhard Rank et al. Identification of a PRMT5-dependent repressor complex linked to silencing of human fetal globin gene expression.Blood 116 (9) 1585-1592 (September 2, 2010)
  11. Marcus J??r??s et al. Isolation and killing of candidate chronic myeloid leukemia stem cells by antibody targeting of IL-1 receptor accessory protein.Proc Natl Acad Sci U S A 107 (37) (August 30, 2010)
  12. Claude Capron et al. A major role of TGF-{beta}1 in the homing capacities of murine hematopoietic stem cell/ progenitorsBlood 116 (8) 1244-1253 (May 20, 2010)
  13. Chinavenmeni S Velu et al. Gfi1 regulates miR-21 and miR-196b to control myelopoiesis.Blood 113 (19) 4720-4728 (May 7, 2009)
  14. Hong Lin et al. Maitake beta-glucan enhances umbilical cord blood stem cell transplantation in the NOD/SCID mouse.Exp Biol Med (Maywood) 234 (3) 342-353 (March 2009)
  15. Ee-Chun Cheng et al. Role for MKL1 in megakaryocytic maturation.Blood 113 (12) 2826-2834 (March 19, 2009)
  16. Michael Milsom et al. Ectopic HOXB4 overcomes the inhibitory effect of tumor necrosis factor-{alpha} on Fanconi anemia hematopoietic stem and progenitor cells.Blood 113 (21) 5111-5120 (March 6, 2009)
  17. Shawn J Chua et al. Neural progenitors, neurons and oligodendrocytes from human umbilical cord blood cells in a serum-free, feeder-free cell culture.Biochem Biophys Res Commun 379 (2) 217-221 (February 6, 2009)
  18. Mette Holm et al. Seven week culture of functional human mast cells from buffy coat preparations.J Immunol Methods 336 (2) 213-221 (July 31, 2008)
  19. Gabriel Ghiaur et al. Rac1 is essential for intraembryonic hematopoiesis and for the initial seeding of fetal liver with definitive hematopoietic progenitor cells.Blood 111 (7) 3313-3321 (April 1, 2008)
  20. Cheng Cheng Zhang et al. Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation.Blood 111 (7) 3415-3423 (January 17, 2008)
  21. Rebecca E Griffiths et al. Normal prion protein trafficking in cultured human erythroblasts.Blood 110 (13) 4518-4525 (December 15, 2007)
  22. Yuh-Ching Twu et al. I branching formation in erythroid differentiation is regulated by transcription factor C/EBPalpha.Blood 110 (13) 4526-4534 (December 15, 2007)
  23. Stefan O Ciurea et al. Pivotal contributions of megakaryocytes to the biology of idiopathic myelofibrosis.Blood 110 (3) 986-993 (August 1, 2007)
  24. Swee Lay Thein et al. Intergenic variants of HBS1L-MYB are responsible for a major quantitative trait locus on chromosome 6q23 influencing fetal hemoglobin levels in adults.Proc Natl Acad Sci U S A 104 (27) 11346-11351 (July 3, 2007)
  25. Iain C Macaulay et al. Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins.Blood 109 (8) 3260-3269 (April 15, 2007)
  26. Shing Leng Chan et al. Enhanced in vivo homing of uncultured and selectively amplified cord blood CD34+ cells by cotransplantation with cord blood-derived unrestricted somatic stem cells.Stem Cells 25 (2) 529-536 (February 2007)
  27. Yasuo Miura et al. Mesenchymal stem cell-organized bone marrow elements: an alternative hematopoietic progenitor resource.Stem Cells 24 (11) 2428-2436 (November 2006)
  28. Alessandra Biffi et al. Gene therapy of metachromatic leukodystrophy reverses neurological damage and deficits in mice.J Clin Invest 116 (11) 3070-3082 (November 2006)
  29. Thomas R Bauer Jr et al. Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.Blood 108 (10) 3313-3320 (November 15, 2006)
  30. Alessandra Mortellaro et al. Ex vivo gene therapy with lentiviral vectors rescues adenosine deaminase (ADA)-deficient mice and corrects their immune and metabolic defects.Blood 108 (9) 2979-2988 (November 1, 2006)
  31. Michael J Nemeth et al. Hmgb3 regulates the balance between hematopoietic stem cell self-renewal and differentiation.Proc Natl Acad Sci U S A 103 (37) 13783-13788 (September 12, 2006)
  32. Jie Jiang et al. cMYB is involved in the regulation of fetal hemoglobin production in adults.Blood 108 (3) 1077-1083 (August 1, 2006)
  33. Selim Corbacioglu et al. Newly identified c-kit receptor tyrosine kinase ITD in childhood AML induces ligand independent growth and is responsive to a synergistic effect of imatinib and rapamycin.Blood 108 (10) 3504-3513 (July 13, 2006)
  34. Joyce S G Yeoh et al. Fibroblast growth factor-1 and -2 preserve long-term repopulating ability of hematopoietic stem cells in serum-free cultures.Stem Cells 24 (6) 1564-1572 (June 2006)
  35. Francesca Romana Santoni de Sio et al. Proteasome activity restricts lentiviral gene transfer into hematopoietic stem cells and is down-regulated by cytokines that enhance transduction.Blood 107 (11) 4257-4265 (June 1, 2006)
  36. David Campard et al. Multilevel regulation of IL-6R by IL-6-sIL-6R fusion protein according to the primitiveness of peripheral blood-derived CD133+ cells.Stem Cells 24 (5) 1302-1314 (May 2006)
  37. Liam O'Mahony et al. Differential cytokine response from dendritic cells to commensal and pathogenic bacteria in different lymphoid compartments in humans.Am J Physiol Gastrointest Liver Physiol 290 (4) G839-45 (April 2006)
  38. Leonie M. Kamminga et al. The Polycomb group gene Ezh2 prevents hematopoietic stem cell exhaustion.Blood 107 (5) 2170-2179 (March 1, 2006)
  39. Wei Zhao et al. Erythropoietin stimulates phosphorylation and activation of GATA-1 via the PI3-kinase/AKT signaling pathway.Blood 107 (3) 907-915 (February 1, 2006)
  40. Ki-Ryang Koh et al. Immunomodulatory derivative of thalidomide (IMiD CC-4047) induces a shift in lineage commitment by suppressing erythropoiesis and promoting myelopoiesis.Blood 105 (10) 3833-3840 (May 15, 2005)
  41. Yaw Ohene-Abuakwa et al. Two-phase culture in Diamond Blackfan anemia: localization of erythroid defect.Blood 105 (2) 838-846 (January 15, 2005)
  42. Cornelia Leberbauer et al. Different steroids co-regulate long-term expansion versus terminal differentiation in primary human erythroid progenitors.Blood 105 (1) 85-94 (January 1, 2005)
  43. Stasia A Anderson et al. Noninvasive MR imaging of magnetically labeled stem cells to directly identify neovasculature in a glioma model.Blood 105 (1) 420-425 (January 1, 2005)
  44. Elena De Falco et al. SDF-1 involvement in endothelial phenotype and ischemia-induced recruitment of bone marrow progenitor cells.Blood 104 (12) 3472-3482 (December 1, 2004)
  45. Qi Wang et al. BUBR1 deficiency results in abnormal megakaryopoiesis.Blood 103 (4) 1278-1285 (February 15, 2004)
  46. Patricia Denning-Kendall et al. Cobblestone area-forming cells in human cord blood are heterogeneous and differ from long-term culture-initiating cells.Stem Cells 21 (6) 694-701 (2003)
  47. Sayed F Abdelwahab et al. HIV-1-suppressive factors are secreted by CD4+ T cells during primary immune responses.Proc Natl Acad Sci U S A 100 (25) 15006-15010 (December 9, 2003)
  48. Frank El-Ouriaghli et al. Clonal dominance of chronic myelogenous leukemia is associated with diminished sensitivity to the antiproliferative effects of neutrophil elastase.Blood 102 (10) 3786-3792 (November 15, 2003)
  49. Xiaoyan Jiang et al. Evidence for a positive role of SHIP in the BCR-ABL-mediated transformation of primitive murine hematopoietic cells and in human chronic myeloid leukemia.Blood 102 (8) 2976-2984 (October 15, 2003)
  50. Guenahel Danet et al. Expansion of human SCID-repopulating cells under hypoxic conditions.The Journal of clinical investigation. 112 (1) 126-135 (July 2003)
  51. Chandini M Thirukkumaran et al. Reovirus oncolysis as a novel purging strategy for autologous stem cell transplantation.Blood 102 (1) 377-387 (July 1, 2003)
  52. I Dybedal et al. Human reconstituting hematopoietic stem cells up-regulate Fas expression upon active cell cycling but remain resistant to Fas-induced suppression.Blood 102 (1) 118-126 (July 1, 2003)
  53. Gunhild Sommer et al. Gastrointestinal stromal tumors in a mouse model by targeted mutation of the Kit receptor tyrosine kinase.Proc Natl Acad Sci U S A 100 (11) 6706-6711 (May 27, 2003)
  54. Enric Esplugues et al. Enhanced antitumor immunity in mice deficient in CD69.J Exp Med 197 (9) 1093-1106 (May 5, 2003)
  55. Didier Bouscary et al. Critical role for PI 3-kinase in the control of erythropoietin-induced erythroid progenitor proliferation.Blood 101 (9) 3436-3443 (May 1, 2003)
  56. Yinghui Guan et al. Detection, isolation, and stimulation of quiescent primitive leukemic progenitor cells from patients with acute myeloid leukemia (AML).Blood 101 (8) 3142-3149 (April 15, 2003)
  57. Niro Inaba et al. A novel I-branching beta-1,6-N-acetylglucosaminyltransferase involved in human blood group I antigen expression.Blood 101 (7) 2870-2876 (April 1, 2003)
  58. Frank El Ouriaghli et al. Neutrophil elastase enzymatically antagonizes the in vitro action of G-CSF: implications for the regulation of granulopoiesis.Blood 101 (5) 1752-1758 (March 1, 2003)
  59. Bernhard Schiedlmeier et al. High-level ectopic HOXB4 expression confers a profound in vivo competitive growth advantage on human cord blood CD34+ cells, but impairs lymphomyeloid differentiation.Blood 101 (5) 1759-1768 (March 1, 2003)
  60. Neeltje A Kootstra et al. Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells.Proc Natl Acad Sci U S A 100 (3) 1298-1303 (February 4, 2003)
  61. Selim Kuci et al. Identification of a novel class of human adherent CD34- stem cells that give rise to SCID-repopulating cells.Blood 101 (3) 869-876 (February 1, 2003)
  62. "Leong et al. Mutant nucleophosmin deregulates cell death and myeloid differentiation through excessive caspase-6 and -8 inhibition.

Background References:

  1. Katrien Vanheusden et al. In vitro expanded cells contributing to rapid severe combined immunodeficient repopulation activity are CD34+38-33+90+45RA-.Stem Cells 25 (1) 107-114 (January 2007)
  2. Evgenios Goussetis et al. Intracoronary infusion of CD133+ and CD133-CD34+ selected autologous bone marrow progenitor cells in patients with chronic ischemic cardiomyopathy: cell isolation, adherence to the infarcted area, and body distribution.Stem Cells 24 (10) 2279-2283 (October 2006)
  3. E Conneally et al. Efficient retroviral-mediated gene transfer to human cord blood stem cells with in vivo repopulating potential.Blood 91 (9) 3487-3493 (May 1, 1998)
  4. C L Miller et al. Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability.Proc Natl Acad Sci U S A 94 (25) 13648-13653 (December 9, 1997)
  5. M E Lemieux et al. Differential ability of flt3-ligand, interleukin-11, and Steel factor to support the generation of B cell progenitors and myeloid cells from primitive murine fetal liver cells.Exp Hematol 25 (9) 951-957 (August 1997)
  6. M Bhatia et al. Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture.J Exp Med 186 (4) 619-624 (August 18, 1997)
  7. A Blair et al. Lack of expression of Thy-1 (CD90) on acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo.Blood 89 (9) 3104-3112 (May 1, 1997)
  8. V I Rebel et al. Amplification of Sca-1+ Lin- WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential.Blood 83 (1) 128-136 (January 1, 1994)
  9. P M Lansdorp et al. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.J Exp Med 175 (6) 1501-1509 (June 1, 1992)

Product Name

Description

Catalog #
Iscove's MDM with 2% FBS Medium for Preparing and Washing Samples for Colony-Forming Cell (CFC) Assays 07700
StemSpan® CC100 Cytokine Cocktail for Expansion of Human Hematopoietic Cells 02690
StemSpan® CC110 Cytokine Cocktail for Expansion of Human Hematopoietic Cells 02697
StemSpan® CC220 Cytokine Cocktail for Expansion of Human Megakaryocyte Progenitors 02696
Human LDL Low Density Lipoproteins Prescreened for Hematopoietic Cell Growth 02698
MethoCult® GF M3434 Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Mouse Cells 03434
MethoCult® H4435 Enriched Methylcellulose Medium with Recombinant Cytokines and EPO for Human Cells 04435
BIT 9500 Serum Substitute Serum Substitute for Culturing Human and Mouse Hematopoietic Cells 09500
HIT Serum Substitute Serum Substitute for Culturing Human Hematopoietic Cells 09550
10% Bovine Serum Albumin in Iscove's MDM
10% Bovine Serum Albumin (BSA) in Iscove's MDM
 09300
10% Human Serum Albumin in Iscove's MDM 10% Human Serum Albumin (HSA) in Iscove's MDM 09350
StemSpan® H3000 Xeno-Free and Serum-Free Medium for Expansion and Culture of Hematopoietic Cells 09800
EasySep® Human CD34 Positive Selection Kit Immunomagnetic Positive Selection Kit 18056
EasySep® Human Whole Blood CD34 Positive Selection Kit Immunomagnetic Positive Selection Kit 18086
EasySep® Human Cord Blood CD34 Positive Selection Kit Immunomagnetic Positive Selection Kit 18096
EasySep® Human Progenitor Cell Enrichment Kit Immunomagnetic Negative Selection Kit 19056
RosetteSep® Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail Immunodensity Negative Selection Cocktail 15027
EasySep® Mouse SCA1 Positive Selection Kit Immunomagnetic Positive Selection Kit 18756
EasySep® Mouse CD117 (cKIT) Positive Selection Kit Immunomagnetic Positive Selection Kit 18757
EasySep® Mouse CD93 (AA4.1) Positive Selection Kit Immunomagnetic Positive Selection Kit 18762
EasySep® Mouse Hematopoietic Progenitor Cell Enrichment Kit Immunomagnetic Negative Selection Kit 19756

Expansion of cord blood CD34+ enriched cells in StemSpan® SFEM and StemSpan® H3000


Expansion of cord blood CD34+ enriched cells in StemSpan® SFEM and StemSpan® H3000
Human cord blood CD34+ enriched cells were cultured for eight days in StemSpan® SFEM and StemSpan® H3000 supplemented with Flt-3 Ligand, Stem Cell Factor, Interleukin-3 and Interleukin-6 (StemSpan® CC100, Catalog #02690). Colony-forming cells (CFCs including BFU-E, CFU-GM and CFU-GEMM) were quantified on the input cell population and cultured using MethoCult® H4435 Enriched (Catalog #04435). Results are expressed as average fold expansion (± 1 standard deviation) of 5 experiments. Note: The magnitude of expansion of hematopoietic cells and progenitors is variable between donors and also dependent on factors including: the phenotype of the input cell population, cytokines and growth supplements used, cell density and time in culture.


Megakaryocyte expansion in StemSpan® SFEM


Megakaryocyte expansion in StemSpan® SFEM
Human cord blood CD34+ enriched cells were cultured in StemSpan® SFEM supplemented with Stem Cell Factor, Thrombopoietin, Interleukin-6 and Interleukin-9 (StemSpan® CC220, Catalog #02696). After 7 days, cells were counted and replated in fresh medium, and cultured for another 7 days. After the second culture period, the fold expansion of total nucleated cells (TNC) and CD41+ megakaryocytes was measured. Results are expressed as average fold expansion (± 1 standard deviation) of 6 experiments.


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