StemSpan® H3000

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Xeno-Free and Serum-Free Medium for Expansion and Culture of Hematopoietic Cells

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  • StemSpan® H3000 100 mL
  • StemSpan® H3000 500 mL
  • Label for StemSpan® H3000 100 mL
  • Label for StemSpan® H3000 500 mL
StemSpan® H3000 100 mL
StemSpan® H3000 has been developed for the in vitro culture and expansion of human hematopoietic cells. StemSpan® H3000 contains pre-tested human-derived and recombinant human proteins. Recombinant cytokines, required for the optimal growth and expansion of hematopoietic cells, have not been added to StemSpan® H3000 medium. This allows users the flexibility to prepare medium that meets their requirements.
Product Name Description Catalog # Size Price Quantity
StemSpan® H3000 Xeno-free and serum-free medium for expansion and culture of human hematopoietic cells 09800 100 mL 95.00 USD      
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StemSpan® H3000 Xeno-free and serum-free medium for expansion and culture of human hematopoietic cells 09850 500 mL 320.00 USD      
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Recommended for:
Culture and expansion of human CD34+ cells and hematopoietic progenitors from bone marrow, mobilized peripheral blood and cord blood
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
StemSpan® H3000 contains only human-derived or recombinant proteins
Product Type: Specialized cell culture media
Application: Expansion
Area of Interest: Cancer, Cord blood banking, Hematopoietic stem cell research, Immunology, Stem cell biology, Transplantation
Cell Type: Hematopoietic stem & progenitor cells
Medium Type: Liquid, Serum-free
Popular Product Line: StemSpan
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

This product has been used in:

  1. Michele S Redell et al. Stat3 signaling in acute myeloid leukemia: ligand-dependent and -independent activation and induction of apoptosis by a novel small-molecule Stat3 inhibitor.Blood 117 (21) 5701-5709 (May 26, 2011)
  2. Hong Lin et al. Maitake beta-glucan enhances umbilical cord blood stem cell transplantation in the NOD/SCID mouse.Exp Biol Med (Maywood) 234 (3) 342-353 (March 2009)
  3. Jonathan M Irish et al. Flt3 Y591 duplication and Bcl-2 overexpression are detected in acute myeloid leukemia cells with high levels of phosphorylated wild-type p53.Blood 109 (6) 2589-2596 (March 15, 2007)
  4. Jielin Zhang et al. Primitive hematopoietic cells resist HIV-1 infection via p21.J Clin Invest 117 (2) 473-481 (February 2007)
  5. Ali S Arbab et al. Magnetic resonance imaging and confocal microscopy studies of magnetically labeled endothelial progenitor cells trafficking to sites of tumor angiogenesis.Stem Cells 24 (3) 671-678 (March 2006)
  6. Dolores Baksh et al. Soluble factor cross-talk between human bone marrow-derived hematopoietic and mesenchymal cells enhances in vitro CFU-F and CFU-O growth and reveals heterogeneity in the mesenchymal progenitor cell compartment.Blood 106 (9) 3012-3019 (November 1, 2005)
  7. J Zhang et al. Silencing p21(Waf1/Cip1/Sdi1) expression increases gene transduction efficiency in primitive human hematopoietic cells.Gene Ther 12 (19) 1444-1452 (October 2005)
  8. Panayotis Ioannidis et al. CRD-BP/IMP1 expression characterizes cord blood CD34+ stem cells and affects c-myc and IGF-II expression in MCF-7 cancer cells.J Biol Chem 280 (20) 20086-20093 (May 20, 2005)
  9. Stasia A Anderson et al. Noninvasive MR imaging of magnetically labeled stem cells to directly identify neovasculature in a glioma model.Blood 105 (1) 420-425 (January 1, 2005)
  10. Bernd Giebel et al. Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells.Blood 104 (8) 2332-2338 (October 15, 2004)
  11. A L Petzer et al. Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.J Exp Med 183 (6) 2551-2558 (June 1, 1996)

Background References:

  1. Katrien Vanheusden et al. In vitro expanded cells contributing to rapid severe combined immunodeficient repopulation activity are CD34+38-33+90+45RA-.Stem Cells 25 (1) 107-114 (January 2007)
  2. Evgenios Goussetis et al. Intracoronary infusion of CD133+ and CD133-CD34+ selected autologous bone marrow progenitor cells in patients with chronic ischemic cardiomyopathy: cell isolation, adherence to the infarcted area, and body distribution.Stem Cells 24 (10) 2279-2283 (October 2006)
  3. E Conneally et al. Efficient retroviral-mediated gene transfer to human cord blood stem cells with in vivo repopulating potential.Blood 91 (9) 3487-3493 (May 1, 1998)
  4. C L Miller et al. Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability.Proc Natl Acad Sci U S A 94 (25) 13648-13653 (December 9, 1997)
  5. M E Lemieux et al. Differential ability of flt3-ligand, interleukin-11, and Steel factor to support the generation of B cell progenitors and myeloid cells from primitive murine fetal liver cells.Exp Hematol 25 (9) 951-957 (August 1997)
  6. M Bhatia et al. Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture.J Exp Med 186 (4) 619-624 (August 18, 1997)
  7. A Blair et al. Lack of expression of Thy-1 (CD90) on acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo.Blood 89 (9) 3104-3112 (May 1, 1997)
  8. V I Rebel et al. Amplification of Sca-1+ Lin- WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential.Blood 83 (1) 128-136 (January 1, 1994)
  9. P M Lansdorp et al. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.J Exp Med 175 (6) 1501-1509 (June 1, 1992)

Product Name

Description

Catalog #
StemSpan® SFEM Serum-Free Medium for Expansion of Hematopoietic Cells 09600
10% Bovine Serum Albumin in Iscove's MDM
10% Bovine Serum Albumin (BSA) in Iscove's MDM
 09300
10% Human Serum Albumin in Iscove's MDM 10% Human Serum Albumin (HSA) in Iscove's MDM 09350
BIT 9500 Serum Substitute Serum Substitute for Culturing Human and Mouse Hematopoietic Cells 09500
HIT Serum Substitute Serum Substitute for Culturing Human Hematopoietic Cells 09550
Iscove's MDM with 2% FBS Medium for Preparing and Washing Samples for Colony-Forming Cell (CFC) Assays 07700
MethoCult® H4435 Enriched Methylcellulose Medium with Recombinant Cytokines and EPO for Human Cells 04435
StemSpan® CC100 Cytokine Cocktail for Expansion of Human Hematopoietic Cells 02690
StemSpan® CC110 Cytokine Cocktail for Expansion of Human Hematopoietic Cells 02697
StemSpan® CC220 Cytokine Cocktail for Expansion of Human Megakaryocyte Progenitors 02696
Human LDL Low Density Lipoproteins Prescreened for Hematopoietic Cell Growth 02698

Expansion of cord blood CD34+ enriched cells in StemSpan® SFEM and StemSpan® H3000


Expansion of cord blood CD34+ enriched cells in StemSpan® SFEM and StemSpan® H3000
Human cord blood CD34+ enriched cells were cultured for eight days in StemSpan® SFEM and StemSpan® H3000 supplemented with Flt-3 Ligand, Stem Cell Factor, Interleukin-3 and Interleukin-6 (StemSpan® CC100, Catalog #02690). Colony-forming cells (CFCs including BFU-E, CFU-GM and CFU-GEMM) were quantified on the input cell population and cultured using MethoCult® H4435 Enriched (Catalog #04435). Results are expressed as average fold expansion (± 1 standard deviation) of 5 experiments. Note: The magnitude of expansion of hematopoietic cells and progenitors is variable between donors and also dependent on factors including: the phenotype of the input cell population, cytokines and growth supplements used, cell density and time in culture.


Importance of Low Density Lipoproteins


Effect of low density lipoproteins (LDLs) on megakaryocyte expansion in StemSpan® H3000 supplemented with StemSpan® CC220 cytokine cocktail
Effect of low density lipoproteins (LDLs) on megakaryocyte expansion in StemSpan® H3000 supplemented with StemSpan® CC220 cytokine cocktail


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