StemSpan® CC220

Recommended for:	Culture and expansion of human megakaryocytic progenitors
Accessory Products:	• StemSpan® SFEM (Catalog #09600/09650) • StemSpan® H3000 (Catalog #09800/09850) • Low Density Lipoproteins, Human (Catalog #02698)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:	• rh Thrombopoietin • rh Stem Cell Factor • rh IL-6 • rh IL-9
Product Type:	Cytokines
Cell Type:	Hematopoietic stem & progenitor cells
Popular Product Line:	StemSpan
Area of Interest:	Hematopoietic stem cell research
Research Area:	Hematopoietic stem cell research

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 02696, Lot# All:29910-PIS_1_0_0.pdf

Educational resources:

•  TECH BULLETIN: Culture of hematopoietic stem and progenitor cells (Catalog #29954)
•  VIDEO: StemSpan® Serum-Free Expansion Media

MSDS:

•  02696-MSDS.pdf

Background References:

1. Katrien Vanheusden et al. In vitro expanded cells contributing to rapid severe combined immunodeficient repopulation activity are CD34+38-33+90+45RA-.Stem Cells 25 (1) 107-114 (January 2007)
2. Evgenios Goussetis et al. Intracoronary infusion of CD133+ and CD133-CD34+ selected autologous bone marrow progenitor cells in patients with chronic ischemic cardiomyopathy: cell isolation, adherence to the infarcted area, and body distribution.Stem Cells 24 (10) 2279-2283 (October 2006)
3. E Conneally et al. Efficient retroviral-mediated gene transfer to human cord blood stem cells with in vivo repopulating potential.Blood 91 (9) 3487-3493 (May 1, 1998)
4. C L Miller et al. Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability.Proc Natl Acad Sci U S A 94 (25) 13648-13653 (December 9, 1997)
5. M E Lemieux et al. Differential ability of flt3-ligand, interleukin-11, and Steel factor to support the generation of B cell progenitors and myeloid cells from primitive murine fetal liver cells.Exp Hematol 25 (9) 951-957 (August 1997)
6. M Bhatia et al. Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture.J Exp Med 186 (4) 619-624 (August 18, 1997)
7. A Blair et al. Lack of expression of Thy-1 (CD90) on acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo.Blood 89 (9) 3104-3112 (May 1, 1997)
8. V I Rebel et al. Amplification of Sca-1+ Lin- WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential.Blood 83 (1) 128-136 (January 1, 1994)
9. P M Lansdorp et al. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.J Exp Med 175 (6) 1501-1509 (June 1, 1992)

Product Name	Description	Catalog #
StemSpan® SFEM	Serum-Free Medium for Expansion of Hematopoietic Cells	09600
StemSpan® H3000	Xeno-Free and Serum-Free Medium for Expansion and Culture of Hematopoietic Cells	09800
StemSpan® CC100	Cytokine Cocktail for Expansion of Human Hematopoietic Cells	02690
StemSpan® CC110	Cytokine Cocktail for Expansion of Human Hematopoietic Cells	02697

Megakaryocyte expansion in StemSpan® SFEM

Human cord blood CD34+ enriched cells were cultured in StemSpan® SFEM supplemented with Stem Cell Factor, Thrombopoietin, Interleukin-6 and Interleukin-9 (StemSpan® CC220, Catalog #02696). After 7 days, cells were counted and replated in fresh medium, and cultured for another 7 days. After the second culture period, the fold expansion of total nucleated cells (TNC) and CD41+ megakaryocytes was measured. Results are expressed as average fold expansion (± 1 standard deviation) of 6 experiments.

Effect of low density lipoproteins (LDLs) on megakaryocyte expansion in StemSpan® H3000 supplemented with StemSpan® CC220 cytokine cocktail