RosetteSep® Anti-Human IgE Tetramer

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Immunodensity Depletion Cocktail

RosetteSep® Box

The RosetteSep® Anti-Human IgE Tetramer Cocktail is designed to deplete IgE-bearing cells (e.g. basophilic and mast cells) from whole blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing IgE and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Ficoll-Paque™ PLUS (Catalog #07957), the unwanted cells pellet along with the RBCs. The purified non-IgE cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.

Product Name	Description	Catalog #	Size	Price	Quantity
RosetteSep® Anti-Human IgE Tetramer	Immunodensity depletion of IgE+ cells	15230	For labeling 40 mL of whole blood	105.00 USD		Save Item

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Recommended for:	The depletion of human IgE+ cells from whole blood
Components:	RosetteSep® Anti-Human IgE Tetramer
Accessory Products:	Ficoll-Paque™ PLUS (Catalog #07957)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Legal Statement:	Ficoll-Paque™ PLUS is a trademark of GE Healthcare Ltd.
Product Type:	Cell isolation products
Cell Type:	Granulocytes & subsets
Popular Product Line:	RosetteSep
Area of Interest:	Immunology
Species:	Human
Cell Source:	Buffy coat, Whole blood
Selection Method:	Negative
Cell Isolation Product Type:	Reagents

Procedures and instruction manuals:

PRODUCT INFORMATION SHEET - 15230, Lot# All:28575-PIS_1_0_0.pdf

Educational resources:

VIDEO: RosetteSep® Introduction
VIDEO: RosetteSep®: A Guide to Isolating NK Cells Directly from Whole Blood
BROCHURE: One-Step Cell Enrichment Directly From Whole Blood
BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
VIDEO: RosetteSep® for HLA Analysis

MSDS:

15230C-MSDS.pdf

FAQS:

Q. WHAT IS ROSETTESEP^®?

A. RosetteSep^® is a rapid cell separation procedure for the isolation of purified cells directly from whole blood without columns or magnets.
Q. HOW DOES ROSETTESEP^® WORK?

A. The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a buoyant density medium.
Q. WHICH CELL SAMPLES CAN ROSETTESEP^® BE USED WITH?

A. RosetteSep^® can be used with leukapheres, bone marrow samples or buffy coat, as long as: the concentration of cells does not exceed 5 x 10⁷ per mL (can dilute if necessary); and there are at least 20 RBCs for every nucleated cell (RBCs can be added if necessary). For a full procedure click here.
Q. CAN ROSETTESEP^® BE USED WITH PREVIOUSLY FROZEN OR CULTURED CELLS?

A. Yes. Cells should be re-suspended at 2 – 5 x 10⁷ cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.
Q. WHICH ANTICOAGULANT SHOULD BE USED WITH ROSETTESEP^®?

A. Peripheral blood should be collected in heparinized Vaccutainers. Cord blood should be collected in ACD.
Q. SHOULD THE ANTICOAGULANT BE WASHED OFF BEFORE USING ROSETTESEP^®?

A. No, the antibody cocktail can be added directly to the sample.
Q. CAN ROSETTESEP^® BE USED TO ENRICH PROGENITORS FROM CORD BLOOD?

A. Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll^TM, which can lead to a higher RBC contamination than peripheral blood separations.
Q. DOES ROSETTESEP^® WORK WITH MOUSE CELLS?

A. No, but we have developed EasySep^®, a magnetic-based separation for the isolation of mouse cells.