RoboSep®

Procedures and instruction manuals:

•  MANUAL: RoboSep® Technical Manual

Educational resources:

•  VIDEO: RoboSep® Product Tour
•  VIDEO: RoboSep® Sneak Preview
•  BROCHURE: RoboSep® Specifications
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  BROCHURE: Cell Separation Products for HLA Analysis
•  BROCHURE: Cell Separation Equipment & Products
•  TECH BULLETIN: Techinical Note: RoboSep® Applications (Catalog #29145)
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  SCIENTIFIC POSTER: Isolation and enrichment of rare cells with an imunomagnetic cell selection system
•  SCIENTIFIC POSTER: Evaluation of the RoboSep Cell Separation for HLA Analysis
•  BROCHURE: RoboSep®: Solutions for HIV Research
•  BROCHURE: Fully Automated, High Purity Cell Isolation for Chimerism Labs
•  TECH BULLETIN: Fully Automated Separation of Multiple Cell Types from a Single Sample
•  WEBINAR: Fully Automated Cell Separation for Chimerism Analysis with RoboSep™

MSDS:

•  20164-MSDS.pdf

This product has been used in:

1. Endi Wang et al. Pseudo-Pelger-Hu??t anomaly induced by medications: a clinicopathologic study in comparison with myelodysplastic syndrome-related pseudo-Pelger-Hu??t anomaly.Am J Clin Pathol 135 (2) 291-303 (February 2011)
2. Pawandeep Dhami et al. Complex exon-intron marking by histone modifications is not determined solely by nucleosome distribution.PLoS One 5 (8) e12339 (2010)
3. Leslie J Marshall et al. Transcription factor Spi-B binds unique sequences present in the tandem repeat promoter/enhancer of JC virus and supports viral activity.J Gen Virol 91 (Pt 12) 3042-3052 (December 2010)
4. Tae-Wook Chun et al. Rebound of plasma viremia following cessation of antiretroviral therapy despite profoundly low levels of HIV reservoir: implications for eradication.AIDS 24 (18) 2803-2808 (November 27, 2010)
5. Rajendra Pahwa et al. Isolation and expansion of human natural T regulatory cells for cellular therapy.J Immunol Methods (October 23, 2010)
6. Sandra Balkow et al. LFA-1 activity state on dendritic cells regulates contact duration with T cells and promotes T-cell priming.Blood 116 (11) 1885-1894 (September 16, 2010)
7. Sven Brode et al. Interleukin-5 inhibits glucocorticoid-mediated apoptosis in human eosinophils.Thorax (August 30, 2010)
8. Andrew S Cowburn et al. GM-CSF causes a Paradoxical Increase in the BH3-only Pro-Apoptotic Protien BIM in Human Neutrophils.Am J Respir Cell Mol Biol (August 12, 2010)
9. Stephen A Mihalcik et al. The structure of the TNFRSF13C promoter enables differential expression of BAFF-R during B cell ontogeny and terminal differentiation.J Immunol 185 (2) 1045-1054 (July 15, 2010)
10. Kei Tomihara et al. Antigen-specific immunity and cross-priming by epithelial ovarian carcinoma-induced CD11b(+)Gr-1(+) cells.J Immunol 184 (11) 6151-6160 (June 1, 2010)
11. Jennifer Crow et al. Donor Cell Leukemia in Umbilical Cord Blood Transplant Patients. A Case Study and Literature Review Highlighting the Importance of Molecular Engraftment Analysis.J Mol Diagn jmoldx.2010.090215 (April 29, 2010)
12. Bernhard Lehnertz et al. Activating and inhibitory functions for the histone lysine methyltransferase G9a in T helper cell differentiation and function.The Journal of Experimental Medicine 207 (5) 915-922 (April 26, 2010)
13. Mathias Krummen et al. Release of IL-12 by dendritic cells activated by TLR ligation is dependent on MyD88 signaling, whereas TRIF signaling is indispensable for TLR synergy.J Leukoc Biol (April 1, 2010)
14. Deepak Rai et al. Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.Proc Natl Acad Sci U S A 107 (7) 3111-3116 (February 16, 2010)
15. Norbert H??ser et al. Intact LFA-1 deactivation promotes T-cell activation and rejection of cardiac allograft.Int Immunol 22 (1) 35-44 (January 2010)
16. Brian P. Doehle et al. Human immunodeficiency virus type 1 mediates global disruption of innate antiviral signaling and immune defenses within infected cells.J. Virol. 83 (20) 10395-10405 (October 15, 2009)
17. Michael L Vetter et al. Cytoplasmic APOBEC3G restricts incoming Vif-positive human immunodeficiency virus type 1 and increases two-long terminal repeat circle formation in activated T-helper-subtype cells.J Virol 83 (17) 8646-8654 (September 2009)
18. Xiao-Nong Wang et al. Regulatory T-cell suppression of CD8+ T-cell-mediated graft-versus-host reaction requires their presence during priming.Transplantation 88 (2) 188-197 (July 27, 2009)
19. Jonathan H Esensten et al. T-bet-deficient NOD mice are protected from diabetes due to defects in both T cell and innate immune system function.J Immunol 183 (1) 75-82 (July 1, 2009)
20. Anna Jankowska et al. Loss of heterozygosity 4q24 and TET2 mutations associated with myelodysplastic/myeloproliferative neoplasmsBlood 113 (25) (June 18, 2009)
21. Sang-Woo Kim et al. Rational combined targeting of phosphodiesterase 4B and SYK in DLBCLBlood 113 (24) (June 11, 2009)
22. Benjamin J Capoccia et al. Revascularization of ischemic limbs after transplantation of human bone marrow cells with high aldehyde dehydrogenase activity.Blood 113 (21) 5340-5351 (May 21, 2009)
23. Nicholas Watkins et al. A HaemAtlas: characterizing gene expression in differentiated human blood cells.Blood 113 (19) e1-9 (May 7, 2009)
24. Roberto Maldonado et al. Control of T helper cell differentiation through cytokine receptor inclusion in the immunological synapseThe Journal of Experimental Medicine 206 (4) (April 6, 2009)
25. V Decot et al. Chimerism analysis following nonmyeloablative stem cell transplantation using a new cell subset separation method: Robosep.Biomed Mater Eng 18 (1 Suppl) S19-26 (2008)
26. Andrew J Dunbar et al. 250K single nucleotide polymorphism array karyotyping identifies acquired uniparental disomy and homozygous mutations, including novel missense substitutions of c-Cbl, in myeloid malignancies.Cancer Res 68 (24) 10349-10357 (December 15, 2008)
27. Xiaosheng Wu et al. Alternative splicing regulates activation-induced cytidine deaminase (AID): implications for suppression of AID mutagenic activity in normal and malignant B cellsBlood 112 (12) (December 1, 2008)
28. Bonnie Arendt et al. Biologic and genetic characterization of the novel amyloidogenic lambda light chain-secreting human cell lines, ALMC-1 and ALMC-2Blood 112 (5) (September 1, 2008)
29. Sook Kyung Chang et al. B Lymphocyte Stimulator Regulates Adaptive Immune Responses by Directly Promoting Dendritic Cell MaturationJ Immunol 180 (11) 7394-7403 (June 1, 2008)
30. Akira Suto et al. Development and characterization of IL-21-producing CD4+ T cells.J. Exp. Med. 205 (6) 1369-20072057 (May 12, 2008)
31. Frances Vu et al. ICOS, CD40, and Lymphotoxin {beta} Receptors Signal Sequentially and Interdependently to Initiate a Germinal Center ReactionJ Immunol 180 (4) 2284-2293 (February 15, 2008)
32. Jaime R. Darce et al. Divergent Effects of BAFF on Human Memory B Cell Differentiation into Ig-Secreting CellsJ Immunol 178 (9) 5612-5622 (May 1, 2007)
33. M Kline et al. ABT-737, an inhibitor of Bcl-2 family proteins, is a potent inducer of apoptosis in multiple myeloma cellsLeukemia 21 (7) 1549-1560 (April 26, 2007)
34. Maureen Fairhurst et al. A Rapid Automated Method for the Sequential Isolation of CD19, CD3 and CD33 cells from One Tube of Whole Blood.The Journal of Immunology 178 (Meeting Abstracts) (April 1, 2007)
35. Ning Yuan et al. A method for rapid isolation of highly purified human monocytes using fully automated negative cell selectionThe Journal of Immunology 178 (Meeting Abstracts) (April 1, 2007)
36. Crescent R Isham et al. Chaetocin: a promising new antimyeloma agent with in vitro and in vivo activity mediated via imposition of oxidative stress.Blood 109 (6) 2579-2588 (March 15, 2007)
37. Andrea J Lengi et al. 17{beta}-Estradiol downregulates interferon regulatory factor-1 in murine splenocytesJ Mol Endocrinol 37 (3) 421-432 (December 1, 2006)
38. M Timm et al. Thymoglobulin targets multiple plasma cell antigens and has in vitro and in vivo activity in multiple myelomaLeukemia 20 (10) 1863-1869 (August 17, 2006)