PneumaCult™-ALI: Culture Human Bronchial Epithelial Cells

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Use PneumaCult™-ALI for Bronchial Epithelial Cells
Use PneumaCult™-ALI for Bronchial Epithelial Cells




PneumaCult™-ALI has been specifically designed to support mucociliary differentiation of primary human bronchial epithelial cells or primary human tracheal epithelial cells in air-liquid interface culture. Human tracheobronchial epithelial cells cultured at the air-liquid interface with PneumaCult™-ALI undergo extensive differentiation to form a pseudostratified epithelium that closely resembles the human airway. Furthermore, PneumaCult™-ALI cultures can elicit physiological responses to known stimuli, making this formulation a robust tool for in vitro lung epithelial cell research. Primary human bronchial epithelial cells differentiated in PneumaCult™-ALI constitute a highly physiological model for respiratory research. Applications include the study of development and disease states of the airway epithelium, toxicity testing and drug transport studies.

Benefits of PneumaCult™-ALI:
  • PHYSIOLOGICALLY RELEVANT: Cells cultured in PneumaCult™-ALI closely model the human airway.
  • REPRODUCIBLE: PneumaCult™-ALI is serum- and BPE-free, maximizing experimental reproducibility.
  • USER-FRIENDLY: Uncomplicated components and optimized protocols make using PneumaCult™-ALI easy.

To learn more about PneumaCult™-ALI, please view our human bronchial epithelial cells webinar.

Air-Liquid Interface Culture of Human Bronchial Epithelial Cells


 
Culturing primary human bronchial or tracheal epithelial cells at the air-liquid interface mimics the in vivo environment and drives differentiation towards a mucociliary phenotype. This culture system is superior to other in vitro models for many research applications because it results in the generation of a more physiologically relevant model. Both immortalized cell lines and primary cells from animals are used for respiratory research, but data generated using these models are not directly applicable to the human system.1 Submerged culture of primary human bronchial epithelial cells or tracheal epithelial cells is possible; however, cells in this system fail to undergo mucociliary differentiation. Air-liquid interface cultures of human bronchial epithelial cells, however, exhibit many of the characteristic properties of the human airway, including mucus secretion, ciliary motility and formation of cellular junctions.

Air-liquid interface culture drives differentiation of bronchial epithelial cells to a pseudostratified mucociliary epithelium.
Schematic of cells grown on cell culture inserts during the submerged phase (A) and following air-lift (B). Air-liquid interface culture drives differentiation of bronchial epithelial cells to a pseudostratified mucociliary epithelium.


1. Bérubé K, et al. Toxicology 278(3): 311-8, 2010

1 products available

Product Name Description Catalog # Size Price Quantity
PneumaCult™-ALI Medium
New Product
Serum- and BPE-free medium for HBECs cultured at ALI 05001 1 Kit 180.00 USD