NeuroCult® NS-A Proliferation Kit (Rat)

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Medium for Expansion of Rat Neural Stem and Progenitor Cells

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  • NeuroCult® NS-A Proliferation Kit (Rat)
  • NeuroCult® NS-A Basal Medium (Rat), 450 mL
  • NeuroCult® NS-A Proliferation Supplement (Rat), 50 mL
NeuroCult® NS-A Proliferation Kit (Rat)
The NeuroCult® NS-A Proliferation Kit (Rat) is a standardized, serum-free medium for the culture of rat neural stem and progenitor cells as neurospheres. The NeuroCult® NS-A Proliferation Kit (Rat) must be supplemented with appropriate cytokines (not included). The medium is optimized to maintain rat neural stem cells in culture for extended periods of time without the loss of their self-renewal, proliferation or differentiation potential. The NeuroCult® NS-A Proliferation Kit (Rat) is comprised of NeuroCult® NS-A Basal Medium (Rat) and NeuroCult® NS-A Proliferation Supplement (Rat).
 
NOTE: Addition of EGF, Human, Recombinant (Catalog #02633), bFGF, Human, Recombinant (Catalog #02634) and Heparin (Catalog #07980) is required.
Product Name Description Catalog # Size Price Quantity
NeuroCult® NS-A Proliferation Kit (Rat) Medium for expansion of rat neural stem and progenitor cells 05771 1 Kit 178.00 USD      
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Recommended for:
• Expansion of embryonic and adult rat neural stem and progenitor cells as neurospheres
• Clonal and bulk isolation and propagation of embryonic and adult rat stem and progenitor cells
• Note: requires supplementation with rh EGF (Catalog #02633), rh bFGF (Catalog #02634) and heparin (Catalog #07980)
Components:
• NeuroCult® NS-A Basal Medium (Rat), 450 mL (Catalog #05770)
• NeuroCult® NS-A Proliferation Supplement (Rat), 50 mL (Catalog #05773)
Accessory Products:
• EGF, Human, Recombinant (Catalog #02633)
• bFGF, Human, Recombinant (Catalog #02634)
• Heparin (Catalog #07980)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
N/A
Legal Statement: Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.
Product Type: Specialized cell culture media
Application: Expansion
Area of Interest: Cancer, Neuroscience, Stem cell biology
Cell Type: Neural stem & progenitor cells
Medium Type: Serum-free, Liquid
Popular Product Line: NeuroCult
Species: Rat

Product Name

Description

Catalog #
NeuroCult® NS-A Differentiation Kit (Rat) Media for Differentiation of Rat Neural Stem and Progenitor Cells 05772
Rat Cortex E18 Neurospheres Cryopreserved Passage 1 Neurospheres from Embryonic Rat Cortical Tissue 00340
Rat Hippocampus E18 Neurospheres Cryopreserved Passage 1 Neurospheres from Embryonic Rat Hippocampal Tissue 00341
Rat Spinal Cord E18 Neurospheres Cryopreserved Passage 1 Neurospheres from Embryonic Rat Spinal Cord Tissue 00342
NeuroCult® NCFC Assay Kit (Rat)
Assay to Quantify Neural Stem and Progenitor Cells
 05742
NeuroCult® Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) Kit for Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue 05715
ALDEFLUOR® Kit ALDEFLUOR® Kit for the Identification, Evaluation and Isolation of Stem and Progenitor Cells Expressing High Levels of ALDH 01700
EGF, Human, Recombinant Epidermal Growth Factor 02633
0.2% Heparin Sodium Salt in PBS 0.2% Heparin Sodium Salt in Phosphate Buffered Saline 07980
bFGF, Human, Recombinant Basic Fibroblast Growth Factor (bFGF) 02634

Comparison of cell expansion and percent viability for rat neurospheres cultured with Complete NeuroCult® Proliferation Medium (Rat) or a traditional formulation cultured in T-25cm2 flasks


Comparison of cell expansion and percent viability for rat neurospheres cultured with Complete NeuroCult® Proliferation Medium (Rat) or a traditional formulation cultured in T-25cm2 flasks
Cells cultured with the NeuroCult® NS-A Proliferation Kit (Rat) (NS-A Medium) continued to generate neurospheres beyond passage 15 resulting in an increase in total cell number. Cells cultured with a traditional formulation did not generate new neurospheres beyond passage 3 when cultured in T-25cm2 flasks.


Comparison of cell expansion and percent viability for rat neurospheres cultured with Complete NeuroCult® Proliferation Medium (Rat) or a traditional formulation cultured in ultra-low adherent dishes


Comparison of cell expansion and percent viability for rat neurospheres cultured with Complete NeuroCult® Proliferation Medium (Rat) or a traditional formulation cultured in ultra-low adherent dishes
Neurospheres could be continually generated in both media formulations beyond passage 10. However, higher cell expansion and percent viability were observed with the NeuroCult® NS-A Proliferation Kit (Rat) (NS-A Medium) compared to a traditional formulation. Neurospheres cultured in ultra-low adherent dishes were very difficult to dissociate after passage 4.


Rat NSCs cultured in NeuroCult® NS-A Proliferation Medium (Rat) maintain multi-lineage potential in long-term cultures and can be differentiated with NeuroCult® Differentiation Medium (Rat) to generate neurons, astrocytes and oligodendrocytes


NSCs cultured in NeuroCult® NS-A Proliferation Medium maintain multi-lineage potential in long-term cultures
Cells obtained from passage 10 neurospheres derived from E18 rat cortical cells cultured in NeuroCult® NS-A Proliferation Medium (Rat) were differentiated with NeuroCult® NS-A Differentiation Medium (Rat). Immunofluorescent staining was performed to identify the differentiated cell types.Neurons (red) were detected with a mouse monoclonal ß-Tubulin III antibody (Catalog #01409), oligodendrocytes (green) with a mouse monoclonal O4 Oligodendrocyte Marker antibody (Catalog #01416) and astrocytes (blue) with a rabbit polyclonal GFAP antibody (Catalog #01415).


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