MammoCult® Human Medium Kit

Recommended for:	• Culture of human mammary epithelial progenitor cells as mammospheres culture • Culture of tumorspheres from human breast cancer cell lines in liquid supension culture
Components:	• MammoCult® Basal Medium (Human), 450mL (Catalog #05621) • MammoCult® Proliferation Supplement (Human), 50mL (Catalog #05622)
Accessory Products:	• Ammonium Chloride Solution (Catalog #07800) • Collagenase/Hyaluronidase Solution (Catalog #07912) • Dispase (5 mg/mL) (Catalog #07913) • DNase I (Catalog #07900) • EpiCult®-B Medium (Catalog #05601) • Fetal Bovine Serum (Catalog #06100 or quality cell culture-tested equivalent) • L-glutamine (Catalgo #07100) • Hanks' Balanced Salt Solution Modified (Catalog #37150) • Heparin (Catalog #07980) • Hydrocortisone (Catalog #07904) • Penicillin-Streptomycin Solution (100X) (Catalog #07500) • Tissue Dissociation Flask (Catalog #27300) • Trypan Blue (Catalog #07050) • Trypsin-EDTA (0.25%) (Catalog #07901) • Ultra-Low Adherent Dishes (5/pack) (Catalog #27145) • 40 µm Cell Strainers (Catalog #27305)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:	N/A
Product Type:	Specialized cell culture media
Application:	Maintenance
Area of Interest:	Cancer, Mammary cell research, Stem cell biology
Cell Type:	Mammary epithelial cells
Medium Type:	Liquid, Serum-free
Popular Product Line:	EpiCult, MammoCult
Species:	Human

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 05620, Lot# All:29569-PIS_1_0_0.pdf

Educational resources:

•  VIDEO: MammoCult® Medium for Culture of Mammospheres and Tumorspheres
•  VIDEO: ALDEFLUOR®: Detect Normal and Cancer Precursor Cells
•  WEBINAR: ALDEFLUOR® and Cancer Stem Cells
•  VIDEO: Tools for Breast Cancer Research
•  BROCHURE: Mammary Epithelial Cells: Standardized Media and Reagents
•  MINI REVIEW: Mini-review: Mammary stem cells (Catalog #29017)
•  TECH BULLETIN: Tumorsphere culture of human breast cancer cell lines (Catalog #29936)
•  BROCHURE: Standardized Solutions for Cancer Stem Cell Research
•  TUTORIAL: Interactive Tutorial - Mammary Epithelial Cell Colony
•  VIDEO: Introducing EpiCult®-C Medium
•  SCIENTIFIC POSTER: Mammosphere Culture Supports Short But Not Long-Term Propagation of Human Mammary Epithelial Progenitors

MSDS:

•  05621-MSDS.pdf
•  05622-MSDS.pdf

This product has been used in:

1. Anupam Kumar et al. Evidence that GTP-binding domain but not catalytic domain of transglutaminase 2 is essential for epithelial-to-mesenchymal transition in mammary epithelial cells.Breast Cancer Res 14 (1) R4 (January 6, 2012)
2. Anupam Kumar et al. Evidence that aberrant expression of tissue transglutaminase promotes stem cell characteristics in mammary epithelial cells.PLoS One 6 (6) e20701 (2011)
3. Caigang Liu et al. Co-expression of Oct-4 and Nestin in human breast cancers.Mol Biol Rep (December 30, 2011)
4. Hong Wu et al. Can CD44+/CD24- Tumor Cells Be Used to Determine the Extent of Breast Cancer Invasion Following Neoadjuvant Chemotherapy?J Breast Cancer 14 (3) 175-180 (September 2011)
5. Cai-Gang Liu et al. Clinical implications of stem cell gene oct-4 expression in breast cancer.Ann Surg 253 (6) 1165-1171 (June 2011)
6. Yuanyuan Qiao et al. FOXQ1 Regulates Epithelial-Mesenchymal Transition in Human Cancers.Cancer Res 71 (8) 3076-3086 (April 15, 2011)
7. Todd W Miller et al. A gene expression signature from human breast cancer cells with acquired hormone independence identifies MYC as a mediator of antiestrogen resistance.Clin Cancer Res 17 (7) 2024-2034 (April 1, 2011)

Background References:

1. Shan Deng et al. Distinct Expression Levels and Patterns of Stem Cell Marker, Aldehyde Dehydrogenase Isoform 1 (ALDH1), in Human Epithelial CancersPLoS One 5 (4) (April 21, 2010)
2. Mark Shackleton et al. Generation of a functional mammary gland from a single stem cell.Nature 439 (7072) 84-88 (January 5, 2006)
3. E C Kordon et al. An entire functional mammary gland may comprise the progeny from a single cell.Development 125 (10) 1921-1930 (May 1998)
4. G H Smith et al. Experimental mammary epithelial morphogenesis in an in vivo model: evidence for distinct cellular progenitors of the ductal and lobular phenotype.Breast Cancer Res Treat 39 (1) 21-31 (1996)

Product Name	Description	Catalog #
EpiCult®-C Human Medium Kit	Medium for Human Mammary Epithelial Cell Culture	05630
EpiCult®-B Medium Kit (Human)	Kit to Culture and Evaluate Human Mammary Epithelial Cells	05601
Collagenase/Hyaluronidase	10X Collagenase/Hyaluronidase in DMEM	07912
Dispase (5 mg/mL)	5 mg/mL Dispase in Hank's Balanced Salt Solution Modified	07913
DNase I Solution (1 mg/mL)	Deoxyribonuclease I Solution (1 mg/mL)	07900
Trypsin-EDTA (0.25%)	Trypsin-EDTA (0.25%)	07901
6-Well Ultra-Low Adherent Plates For Suspension Cultures	Ultra-Low Adherent 6 Well Plates	27145
Hydrocortisone	Hydrocortisone	07904
0.2% Heparin Sodium Salt in PBS	0.2% Heparin Sodium Salt in Phosphate Buffered Saline	07980
ALDEFLUOR® Kit	ALDEFLUOR® Kit for the Identification, Evaluation and Isolation of Stem and Progenitor Cells Expressing High Levels of ALDH	01700
40 µm Cell Strainer	40 µm Nylon Cell Strainer	27305
Mammary Stem Cell Training Course	Training Course for Mammary Stem Cells	00212

Protocol for isolation and identification of human and mouse mammary epithelial progenitor cells

Phase contrast photographs of (A) a pure human myoepithelial cell colony, (B) a pure human luminal cell colony, and (C) a mixed human colony. (D) is a mouse colony. Unlike human mammary CFC colonies, subtypes of mouse mammary epithelial cell colonies are not easily identifiable. All colonies were cultured in either EpiCult®-B (Human: Catalog #05601) or EpiCult®-B (Mouse:Catalog #5610) in the presence of an irradiated NIH 3T3 feeder layer. Colonies were visualized by staining with Wright's Giemsa. (E) is a picture of mammospheres obtained from primary human mammary epithelial cells and (F) is an image of tumorspheres obtained from MCF7 human breast cancer cell line.